Biocompatibility and mineralization activity of modified glass ionomer cement in human dental pulp stem cells
Background/purpose: Fortilin is a multi-functional protein involved in several cellular processes. It has been shown promising potential to be a bioactive molecule that can be incorporated in the dental materials. This study aimed to compare the biocompatibility and mineralization activities of modi...
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Format: | Article |
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Elsevier
2023-07-01
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Series: | Journal of Dental Sciences |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S1991790222003087 |
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author | Apisit Chumpraman Sissada Tannukit Wilaiwan Chotigeat Ureporn Kedjarune-Leggat |
author_facet | Apisit Chumpraman Sissada Tannukit Wilaiwan Chotigeat Ureporn Kedjarune-Leggat |
author_sort | Apisit Chumpraman |
collection | DOAJ |
description | Background/purpose: Fortilin is a multi-functional protein involved in several cellular processes. It has been shown promising potential to be a bioactive molecule that can be incorporated in the dental materials. This study aimed to compare the biocompatibility and mineralization activities of modified glass ionomer cement (Bio-GIC) and Biodentine by direct and indirect method on human dental pulp stem cells (hDPSCs). Materials and methods: Conventional glass ionomer cement (GIC), Bio-GIC (GIC supplemented with chitosan, tricalcium phosphate, and recombinant fortilin from Fenneropenaeus merguiensis), and Biodentine were examined in this study. Recombinant fortilin was purified and tested for its cytotoxicity by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assay. Human DPSCs were treated with different material eluate for particular time intervals. At given time points, viability of hDPSCs was examined using MTT assay and calcium deposition was assessed by Alizarin red staining assay. Comparisons of the data among groups were analyzed by analysis of variance and Tukey's multiple comparisons. Results: All test materials demonstrated no cytotoxicity. In addition, Bio-GIC promoted cell proliferation at 72 h. For direct and indirect method, cells treated with Bio-GIC demonstrated significantly higher calcium deposition than other groups (P < 0.05). Conclusion: Bio-GIC and Biodentine are not cytotoxic to hDPSCs. Bio-GIC demonstrates enhanced calcium deposition comparable to Biodentine. Bio-GIC may be further developed as a bioactive material for dentin regeneration. |
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id | doaj.art-5914dc0357a5414eb6f80e2a554136b5 |
institution | Directory Open Access Journal |
issn | 1991-7902 |
language | English |
last_indexed | 2024-03-13T06:38:01Z |
publishDate | 2023-07-01 |
publisher | Elsevier |
record_format | Article |
series | Journal of Dental Sciences |
spelling | doaj.art-5914dc0357a5414eb6f80e2a554136b52023-06-09T04:27:28ZengElsevierJournal of Dental Sciences1991-79022023-07-0118310551061Biocompatibility and mineralization activity of modified glass ionomer cement in human dental pulp stem cellsApisit Chumpraman0Sissada Tannukit1Wilaiwan Chotigeat2Ureporn Kedjarune-Leggat3Department of Oral Biology and Occlusion, Faculty of Dentistry, Prince of Songkla University, Songkhla, ThailandDepartment of Oral Biology and Occlusion, Faculty of Dentistry, Prince of Songkla University, Songkhla, Thailand; Cell Biology and Biomaterial Research Unit, Faculty of Dentistry, Prince of Songkla University, Songkhla, Thailand; Corresponding author. Department of Oral Biology and Occlusion, Faculty of Dentistry, Prince of Songkla University, 15 Kanchanavanich Rd., Hat Yai, Songkhla, 90110, Thailand.Biological Science Division, Faculty of Science, Prince of Songkla University, Songkhla, ThailandDepartment of Oral Biology and Occlusion, Faculty of Dentistry, Prince of Songkla University, Songkhla, Thailand; Cell Biology and Biomaterial Research Unit, Faculty of Dentistry, Prince of Songkla University, Songkhla, ThailandBackground/purpose: Fortilin is a multi-functional protein involved in several cellular processes. It has been shown promising potential to be a bioactive molecule that can be incorporated in the dental materials. This study aimed to compare the biocompatibility and mineralization activities of modified glass ionomer cement (Bio-GIC) and Biodentine by direct and indirect method on human dental pulp stem cells (hDPSCs). Materials and methods: Conventional glass ionomer cement (GIC), Bio-GIC (GIC supplemented with chitosan, tricalcium phosphate, and recombinant fortilin from Fenneropenaeus merguiensis), and Biodentine were examined in this study. Recombinant fortilin was purified and tested for its cytotoxicity by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assay. Human DPSCs were treated with different material eluate for particular time intervals. At given time points, viability of hDPSCs was examined using MTT assay and calcium deposition was assessed by Alizarin red staining assay. Comparisons of the data among groups were analyzed by analysis of variance and Tukey's multiple comparisons. Results: All test materials demonstrated no cytotoxicity. In addition, Bio-GIC promoted cell proliferation at 72 h. For direct and indirect method, cells treated with Bio-GIC demonstrated significantly higher calcium deposition than other groups (P < 0.05). Conclusion: Bio-GIC and Biodentine are not cytotoxic to hDPSCs. Bio-GIC demonstrates enhanced calcium deposition comparable to Biodentine. Bio-GIC may be further developed as a bioactive material for dentin regeneration.http://www.sciencedirect.com/science/article/pii/S1991790222003087FortilinCytotoxicityMineralizationDental materials |
spellingShingle | Apisit Chumpraman Sissada Tannukit Wilaiwan Chotigeat Ureporn Kedjarune-Leggat Biocompatibility and mineralization activity of modified glass ionomer cement in human dental pulp stem cells Journal of Dental Sciences Fortilin Cytotoxicity Mineralization Dental materials |
title | Biocompatibility and mineralization activity of modified glass ionomer cement in human dental pulp stem cells |
title_full | Biocompatibility and mineralization activity of modified glass ionomer cement in human dental pulp stem cells |
title_fullStr | Biocompatibility and mineralization activity of modified glass ionomer cement in human dental pulp stem cells |
title_full_unstemmed | Biocompatibility and mineralization activity of modified glass ionomer cement in human dental pulp stem cells |
title_short | Biocompatibility and mineralization activity of modified glass ionomer cement in human dental pulp stem cells |
title_sort | biocompatibility and mineralization activity of modified glass ionomer cement in human dental pulp stem cells |
topic | Fortilin Cytotoxicity Mineralization Dental materials |
url | http://www.sciencedirect.com/science/article/pii/S1991790222003087 |
work_keys_str_mv | AT apisitchumpraman biocompatibilityandmineralizationactivityofmodifiedglassionomercementinhumandentalpulpstemcells AT sissadatannukit biocompatibilityandmineralizationactivityofmodifiedglassionomercementinhumandentalpulpstemcells AT wilaiwanchotigeat biocompatibilityandmineralizationactivityofmodifiedglassionomercementinhumandentalpulpstemcells AT urepornkedjaruneleggat biocompatibilityandmineralizationactivityofmodifiedglassionomercementinhumandentalpulpstemcells |