Isolation and Cultivation of Human Gut Microorganisms: A Review
Microbial resources from the human gut may find use in various applications, such as empirical research on the microbiome, the development of probiotic products, and bacteriotherapy. Due to the development of “culturomics”, the number of pure bacterial cultures obtained from the human gut has signif...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/4/1080 |
| _version_ | 1797604164397694976 |
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| author | Xuchun Wan Qianqian Yang Xiangfeng Wang Yun Bai Zhi Liu |
| author_facet | Xuchun Wan Qianqian Yang Xiangfeng Wang Yun Bai Zhi Liu |
| author_sort | Xuchun Wan |
| collection | DOAJ |
| description | Microbial resources from the human gut may find use in various applications, such as empirical research on the microbiome, the development of probiotic products, and bacteriotherapy. Due to the development of “culturomics”, the number of pure bacterial cultures obtained from the human gut has significantly increased since 2012. However, there is still a considerable number of human gut microbes to be isolated and cultured. Thus, to improve the efficiency of obtaining microbial resources from the human gut, some constraints of the current methods, such as labor burden, culture condition, and microbial targetability, still need to be optimized. Here, we overview the general knowledge and recent development of culturomics for human gut microorganisms. Furthermore, we discuss the optimization of several parts of culturomics including sample collection, sample processing, isolation, and cultivation, which may improve the current strategies. |
| first_indexed | 2024-03-11T04:42:30Z |
| format | Article |
| id | doaj.art-5915ad45d4f847cea0e80f77de315b07 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T04:42:30Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-5915ad45d4f847cea0e80f77de315b072023-11-17T20:34:42ZengMDPI AGMicroorganisms2076-26072023-04-01114108010.3390/microorganisms11041080Isolation and Cultivation of Human Gut Microorganisms: A ReviewXuchun Wan0Qianqian Yang1Xiangfeng Wang2Yun Bai3Zhi Liu4Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaDepartment of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaDepartment of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaDepartment of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaDepartment of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaMicrobial resources from the human gut may find use in various applications, such as empirical research on the microbiome, the development of probiotic products, and bacteriotherapy. Due to the development of “culturomics”, the number of pure bacterial cultures obtained from the human gut has significantly increased since 2012. However, there is still a considerable number of human gut microbes to be isolated and cultured. Thus, to improve the efficiency of obtaining microbial resources from the human gut, some constraints of the current methods, such as labor burden, culture condition, and microbial targetability, still need to be optimized. Here, we overview the general knowledge and recent development of culturomics for human gut microorganisms. Furthermore, we discuss the optimization of several parts of culturomics including sample collection, sample processing, isolation, and cultivation, which may improve the current strategies.https://www.mdpi.com/2076-2607/11/4/1080gut microbeshumanculturomicstargeted sortingenrichmentuncultured microbes |
| spellingShingle | Xuchun Wan Qianqian Yang Xiangfeng Wang Yun Bai Zhi Liu Isolation and Cultivation of Human Gut Microorganisms: A Review Microorganisms gut microbes human culturomics targeted sorting enrichment uncultured microbes |
| title | Isolation and Cultivation of Human Gut Microorganisms: A Review |
| title_full | Isolation and Cultivation of Human Gut Microorganisms: A Review |
| title_fullStr | Isolation and Cultivation of Human Gut Microorganisms: A Review |
| title_full_unstemmed | Isolation and Cultivation of Human Gut Microorganisms: A Review |
| title_short | Isolation and Cultivation of Human Gut Microorganisms: A Review |
| title_sort | isolation and cultivation of human gut microorganisms a review |
| topic | gut microbes human culturomics targeted sorting enrichment uncultured microbes |
| url | https://www.mdpi.com/2076-2607/11/4/1080 |
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