Association of CYP gene polymorphisms with breast cancer risk and prognostic factors in the Jordanian population
Abstract Background Single nucleotide polymorphisms (SNPs) in several CYP genes have been associated with altered breast cancer (BC) risk in different populations. Despite this, there is a dearth of information on the roles of these SNPs in Jordanian BC patients. Therefore, this study aims to determ...
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| Format: | Article |
| Language: | English |
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BMC
2019-09-01
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| Series: | BMC Medical Genetics |
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| Online Access: | http://link.springer.com/article/10.1186/s12881-019-0884-x |
| _version_ | 1818644547145564160 |
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| author | Laith N. AL-Eitan Doaa M. Rababa’h Mansour A. Alghamdi Rame H. Khasawneh |
| author_facet | Laith N. AL-Eitan Doaa M. Rababa’h Mansour A. Alghamdi Rame H. Khasawneh |
| author_sort | Laith N. AL-Eitan |
| collection | DOAJ |
| description | Abstract Background Single nucleotide polymorphisms (SNPs) in several CYP genes have been associated with altered breast cancer (BC) risk in different populations. Despite this, there is a dearth of information on the roles of these SNPs in Jordanian BC patients. Therefore, this study aims to determine if there is any single nucleotide polymorphism (SNP) within CYP19A1, CYP2C19, CYP2C9, CYP1B1, CYP3A4, and CYP1A2 genes associated with BC in the Jordanian population. In addition, this work investigates the association between selected BC prognostic factors and variants of the aforementioned CYP candidate genes. Methods Blood samples were withdrawn from 221 BC patients and 218 healthy volunteers recruited from the Jordanian population. Genomic DNA was withdrawn and, after quantification and quality control, was genotyped using the Sequenom MassARRAY® system (iPLEX GOLD). Statistical analysis was then carried out to assess allelic and genotypic frequencies as well as genetic association between cases and controls. Results The CYP19A1 SNP rs7176005 (p < 0.0045) and the CYP1A2 SNP rs762551 (p = 0.004) were significantly associated with BC risk. However, no such association was found for the screened SNPs of the CYP2C9, CYP1B1, CYP2C19 and CYP3A4 genes. Regarding the prognostic factors of BC, several of the screened SNPs were associated with different pathological and clinical features. Conclusions Certain CYP genes, particularly CYP19A1 and CYP1A2, were associated with BC risk and development in the Jordanian population. |
| first_indexed | 2024-12-17T00:16:35Z |
| format | Article |
| id | doaj.art-5916dacd0eca49a4b90ee95dad9e0ea7 |
| institution | Directory Open Access Journal |
| issn | 1471-2350 |
| language | English |
| last_indexed | 2024-12-17T00:16:35Z |
| publishDate | 2019-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Medical Genetics |
| spelling | doaj.art-5916dacd0eca49a4b90ee95dad9e0ea72022-12-21T22:10:42ZengBMCBMC Medical Genetics1471-23502019-09-0120111210.1186/s12881-019-0884-xAssociation of CYP gene polymorphisms with breast cancer risk and prognostic factors in the Jordanian populationLaith N. AL-Eitan0Doaa M. Rababa’h1Mansour A. Alghamdi2Rame H. Khasawneh3Department of Applied Biological Sciences, Jordan University of Science and TechnologyDepartment of Applied Biological Sciences, Jordan University of Science and TechnologyCollege of Medicine, King Khalid UniversityDepartment of Hematopathology, King Hussein Medical Center (KHMC), Jordan Royal Medical Services (RMS)Abstract Background Single nucleotide polymorphisms (SNPs) in several CYP genes have been associated with altered breast cancer (BC) risk in different populations. Despite this, there is a dearth of information on the roles of these SNPs in Jordanian BC patients. Therefore, this study aims to determine if there is any single nucleotide polymorphism (SNP) within CYP19A1, CYP2C19, CYP2C9, CYP1B1, CYP3A4, and CYP1A2 genes associated with BC in the Jordanian population. In addition, this work investigates the association between selected BC prognostic factors and variants of the aforementioned CYP candidate genes. Methods Blood samples were withdrawn from 221 BC patients and 218 healthy volunteers recruited from the Jordanian population. Genomic DNA was withdrawn and, after quantification and quality control, was genotyped using the Sequenom MassARRAY® system (iPLEX GOLD). Statistical analysis was then carried out to assess allelic and genotypic frequencies as well as genetic association between cases and controls. Results The CYP19A1 SNP rs7176005 (p < 0.0045) and the CYP1A2 SNP rs762551 (p = 0.004) were significantly associated with BC risk. However, no such association was found for the screened SNPs of the CYP2C9, CYP1B1, CYP2C19 and CYP3A4 genes. Regarding the prognostic factors of BC, several of the screened SNPs were associated with different pathological and clinical features. Conclusions Certain CYP genes, particularly CYP19A1 and CYP1A2, were associated with BC risk and development in the Jordanian population.http://link.springer.com/article/10.1186/s12881-019-0884-xBreast cancerPrognostic factorsCYPCYP19A1CYP1A |
| spellingShingle | Laith N. AL-Eitan Doaa M. Rababa’h Mansour A. Alghamdi Rame H. Khasawneh Association of CYP gene polymorphisms with breast cancer risk and prognostic factors in the Jordanian population BMC Medical Genetics Breast cancer Prognostic factors CYP CYP19A1 CYP1A |
| title | Association of CYP gene polymorphisms with breast cancer risk and prognostic factors in the Jordanian population |
| title_full | Association of CYP gene polymorphisms with breast cancer risk and prognostic factors in the Jordanian population |
| title_fullStr | Association of CYP gene polymorphisms with breast cancer risk and prognostic factors in the Jordanian population |
| title_full_unstemmed | Association of CYP gene polymorphisms with breast cancer risk and prognostic factors in the Jordanian population |
| title_short | Association of CYP gene polymorphisms with breast cancer risk and prognostic factors in the Jordanian population |
| title_sort | association of cyp gene polymorphisms with breast cancer risk and prognostic factors in the jordanian population |
| topic | Breast cancer Prognostic factors CYP CYP19A1 CYP1A |
| url | http://link.springer.com/article/10.1186/s12881-019-0884-x |
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