Capicua Regulates Dendritic Morphogenesis Through Ets in Hippocampal Neurons in vitro
Capicua (Cic), a transcriptional repressor frequently mutated in brain cancer oligodendroglioma, is highly expressed in adult neurons. However, its function in the dendritic growth of neurons in the hippocampus remains poorly understood. Here, we confirmed that Cic was expressed in hippocampal neuro...
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| Language: | English |
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Neuroanatomy |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnana.2021.669310/full |
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| author | Keqin Li Shuai Shao Tongjie Ji Min Liu Lufeng Wang Ying Pang Mu Chen Siyi Xu Kuiming Zhang Qi Wang Zhongwei Zhuang Liang Wei Yanfei Zhang Yanlin Chen Yang Wang Jing Zhang Kui Chen Hao Lian Chunlong Zhong |
| author_facet | Keqin Li Shuai Shao Tongjie Ji Min Liu Lufeng Wang Ying Pang Mu Chen Siyi Xu Kuiming Zhang Qi Wang Zhongwei Zhuang Liang Wei Yanfei Zhang Yanlin Chen Yang Wang Jing Zhang Kui Chen Hao Lian Chunlong Zhong |
| author_sort | Keqin Li |
| collection | DOAJ |
| description | Capicua (Cic), a transcriptional repressor frequently mutated in brain cancer oligodendroglioma, is highly expressed in adult neurons. However, its function in the dendritic growth of neurons in the hippocampus remains poorly understood. Here, we confirmed that Cic was expressed in hippocampal neurons during the main period of dendritogenesis, suggesting that Cic has a function in dendrite growth. Loss-of-function and gain-of function assays indicated that Cic plays a central role in the inhibition of dendritic morphogenesis and dendritic spines in vitro. Further studies showed that overexpression of Cic reduced the expression of Ets in HT22 cells, while in vitro knockdown of Cic in hippocampal neurons significantly elevated the expression of Ets. These results suggest that Cic may negatively control dendrite growth through Ets, which was confirmed by ShRNA knockdown of either Etv4 or Etv5 abolishing the phenotype of Cic knockdown in cultured neurons. Taken together, our results suggest that Cic inhibits dendritic morphogenesis and the growth of dendritic spines through Ets. |
| first_indexed | 2024-12-22T11:01:23Z |
| format | Article |
| id | doaj.art-5918075d2328459e9ed8f90301d4fe9e |
| institution | Directory Open Access Journal |
| issn | 1662-5129 |
| language | English |
| last_indexed | 2024-12-22T11:01:23Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroanatomy |
| spelling | doaj.art-5918075d2328459e9ed8f90301d4fe9e2022-12-21T18:28:29ZengFrontiers Media S.A.Frontiers in Neuroanatomy1662-51292021-07-011510.3389/fnana.2021.669310669310Capicua Regulates Dendritic Morphogenesis Through Ets in Hippocampal Neurons in vitroKeqin Li0Shuai Shao1Tongjie Ji2Min Liu3Lufeng Wang4Ying Pang5Mu Chen6Siyi Xu7Kuiming Zhang8Qi Wang9Zhongwei Zhuang10Liang Wei11Yanfei Zhang12Yanlin Chen13Yang Wang14Jing Zhang15Kui Chen16Hao Lian17Chunlong Zhong18Department of Neurosurgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Neurosurgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Neurosurgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Neurosurgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Neurology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Neurosurgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Neurosurgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Neurosurgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Neurosurgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Neurosurgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Neurosurgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Neurosurgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Neurosurgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Neurosurgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Emergency, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Neurosurgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Neurosurgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Neurosurgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Neurosurgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaCapicua (Cic), a transcriptional repressor frequently mutated in brain cancer oligodendroglioma, is highly expressed in adult neurons. However, its function in the dendritic growth of neurons in the hippocampus remains poorly understood. Here, we confirmed that Cic was expressed in hippocampal neurons during the main period of dendritogenesis, suggesting that Cic has a function in dendrite growth. Loss-of-function and gain-of function assays indicated that Cic plays a central role in the inhibition of dendritic morphogenesis and dendritic spines in vitro. Further studies showed that overexpression of Cic reduced the expression of Ets in HT22 cells, while in vitro knockdown of Cic in hippocampal neurons significantly elevated the expression of Ets. These results suggest that Cic may negatively control dendrite growth through Ets, which was confirmed by ShRNA knockdown of either Etv4 or Etv5 abolishing the phenotype of Cic knockdown in cultured neurons. Taken together, our results suggest that Cic inhibits dendritic morphogenesis and the growth of dendritic spines through Ets.https://www.frontiersin.org/articles/10.3389/fnana.2021.669310/fullcapicuatranscriptional repressordendritic growthspine growthEts factors |
| spellingShingle | Keqin Li Shuai Shao Tongjie Ji Min Liu Lufeng Wang Ying Pang Mu Chen Siyi Xu Kuiming Zhang Qi Wang Zhongwei Zhuang Liang Wei Yanfei Zhang Yanlin Chen Yang Wang Jing Zhang Kui Chen Hao Lian Chunlong Zhong Capicua Regulates Dendritic Morphogenesis Through Ets in Hippocampal Neurons in vitro Frontiers in Neuroanatomy capicua transcriptional repressor dendritic growth spine growth Ets factors |
| title | Capicua Regulates Dendritic Morphogenesis Through Ets in Hippocampal Neurons in vitro |
| title_full | Capicua Regulates Dendritic Morphogenesis Through Ets in Hippocampal Neurons in vitro |
| title_fullStr | Capicua Regulates Dendritic Morphogenesis Through Ets in Hippocampal Neurons in vitro |
| title_full_unstemmed | Capicua Regulates Dendritic Morphogenesis Through Ets in Hippocampal Neurons in vitro |
| title_short | Capicua Regulates Dendritic Morphogenesis Through Ets in Hippocampal Neurons in vitro |
| title_sort | capicua regulates dendritic morphogenesis through ets in hippocampal neurons in vitro |
| topic | capicua transcriptional repressor dendritic growth spine growth Ets factors |
| url | https://www.frontiersin.org/articles/10.3389/fnana.2021.669310/full |
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