LAMP5 may promote MM progression by activating p38
Multiple myeloma (MM) is the second most common tumor of the hematologic system. MM remains incurable at this time. In this study, we used bioinformatics analysis to find key genes in the pathogenesis of MM. We first found that Lysosome associated membrane protein 5 (LAMP5) expression was sequential...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Pathology and Oncology Research |
| Subjects: | |
| Online Access: | https://www.por-journal.com/articles/10.3389/pore.2023.1611083/full |
| _version_ | 1827290261896560640 |
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| author | Yan Chen Tao Ma |
| author_facet | Yan Chen Tao Ma |
| author_sort | Yan Chen |
| collection | DOAJ |
| description | Multiple myeloma (MM) is the second most common tumor of the hematologic system. MM remains incurable at this time. In this study, we used bioinformatics analysis to find key genes in the pathogenesis of MM. We first found that Lysosome associated membrane protein 5 (LAMP5) expression was sequentially increased in healthy donors (HD), monoclonal gammopathy of undetermined significance (MGUS), smoldering multiple myeloma (SMM) and newly diagnosed MM (NDMM), relapsed MM (RMM). We collected bone marrow from patients with NDMM, HD and post-treatment MM (PTMM) and performed qPCR analysis of LAMP5, and found that the expression of LAMP5 is stronger in NDMM than in HD, and decreases after treatment. Western blotting assay also found more expression of LAMP5 in NDMM than in HD. Patients with high LAMP5 expression have a higher DS (Durie-Salmon) stage and worse prognosis. We next verified the expression of LAMP5 in four MM cell lines and silenced LAMP5 expression in RPMI-8226 and AMO-1, and explored the effects of LAMP5 silencing on MM cell apoptosis and cell cycle by flow cytometry and western blotting. Knockdown of LAMP5 promoted apoptosis in MM cells, but had no effect on the cell cycle. Mechanistically, LAMP5 may exert its pro-tumor effects in MM in part through activation of p38 protein. We screened LAMP5 for the first time as a key gene for MM progression and recurrence, and found that LAMP5 may exert its pro-tumor effects in MM through activation of p38 protein. |
| first_indexed | 2024-04-24T12:13:32Z |
| format | Article |
| id | doaj.art-591d5e464d1443ea99584ab555bbf0c2 |
| institution | Directory Open Access Journal |
| issn | 1532-2807 |
| language | English |
| last_indexed | 2024-04-24T12:13:32Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Pathology and Oncology Research |
| spelling | doaj.art-591d5e464d1443ea99584ab555bbf0c22024-04-08T09:09:28ZengFrontiers Media S.A.Pathology and Oncology Research1532-28072023-03-012910.3389/pore.2023.16110831611083LAMP5 may promote MM progression by activating p38Yan ChenTao MaMultiple myeloma (MM) is the second most common tumor of the hematologic system. MM remains incurable at this time. In this study, we used bioinformatics analysis to find key genes in the pathogenesis of MM. We first found that Lysosome associated membrane protein 5 (LAMP5) expression was sequentially increased in healthy donors (HD), monoclonal gammopathy of undetermined significance (MGUS), smoldering multiple myeloma (SMM) and newly diagnosed MM (NDMM), relapsed MM (RMM). We collected bone marrow from patients with NDMM, HD and post-treatment MM (PTMM) and performed qPCR analysis of LAMP5, and found that the expression of LAMP5 is stronger in NDMM than in HD, and decreases after treatment. Western blotting assay also found more expression of LAMP5 in NDMM than in HD. Patients with high LAMP5 expression have a higher DS (Durie-Salmon) stage and worse prognosis. We next verified the expression of LAMP5 in four MM cell lines and silenced LAMP5 expression in RPMI-8226 and AMO-1, and explored the effects of LAMP5 silencing on MM cell apoptosis and cell cycle by flow cytometry and western blotting. Knockdown of LAMP5 promoted apoptosis in MM cells, but had no effect on the cell cycle. Mechanistically, LAMP5 may exert its pro-tumor effects in MM in part through activation of p38 protein. We screened LAMP5 for the first time as a key gene for MM progression and recurrence, and found that LAMP5 may exert its pro-tumor effects in MM through activation of p38 protein.https://www.por-journal.com/articles/10.3389/pore.2023.1611083/fullapoptosisrecurrenceprognosismultiple myelomaLAMP5 |
| spellingShingle | Yan Chen Tao Ma LAMP5 may promote MM progression by activating p38 Pathology and Oncology Research apoptosis recurrence prognosis multiple myeloma LAMP5 |
| title | LAMP5 may promote MM progression by activating p38 |
| title_full | LAMP5 may promote MM progression by activating p38 |
| title_fullStr | LAMP5 may promote MM progression by activating p38 |
| title_full_unstemmed | LAMP5 may promote MM progression by activating p38 |
| title_short | LAMP5 may promote MM progression by activating p38 |
| title_sort | lamp5 may promote mm progression by activating p38 |
| topic | apoptosis recurrence prognosis multiple myeloma LAMP5 |
| url | https://www.por-journal.com/articles/10.3389/pore.2023.1611083/full |
| work_keys_str_mv | AT yanchen lamp5maypromotemmprogressionbyactivatingp38 AT taoma lamp5maypromotemmprogressionbyactivatingp38 |