Cryopreservation of Aceh Cattle Semen with Date (Phoenix dactylifera) Extract Supplementation
Cryopreservation process could affect spermatozoa quality during from reactive oxygen species (ROS) produced in cellular metabolism and the environment. Spermatozoa damage caused by ROS during cryopreservation can be reduced with the addition of natural antioxidant which commonly found in fruits lik...
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| Format: | Article |
| Language: | English |
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Universitas Negeri Semarang
2019-04-01
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| Series: | Biosaintifika: Journal of Biology & Biology Education |
| Subjects: | |
| Online Access: | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/18033 |
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| author | Yonadiah Dwitya Kartini Eriani Hendra Saputra Al-Azhar Al-Azhar Muhammad Rizal |
| author_facet | Yonadiah Dwitya Kartini Eriani Hendra Saputra Al-Azhar Al-Azhar Muhammad Rizal |
| author_sort | Yonadiah Dwitya |
| collection | DOAJ |
| description | Cryopreservation process could affect spermatozoa quality during from reactive oxygen species (ROS) produced in cellular metabolism and the environment. Spermatozoa damage caused by ROS during cryopreservation can be reduced with the addition of natural antioxidant which commonly found in fruits like date palm. This research was done to investigate the influence of date extract on semen quality after cryopreservation. This experimental study used a completely randomized design with 4 treatments and 6 replications. Semen collected from two aceh cattle bulls was diluted in tris egg yolk extender contained different concentrations (v/v) of date extract: 0% (P0, control), 0.75% (P1), 1% (P2), and 1.25% (P3) before cryopreserved at -196 ºC for 7 days. Semen quality prior to and after cryopreservation as well as sperm DNA integrity were determined by standard microscopic and laddering methods, respectively. The results showed that the addition of 1% date extract could maintain viability (68.67%), plasma membrane integrity (62.33%), and abnormality (18.58%) of aceh cattle spermatozoa, but unable to maintain its motility above 40%. There was no DNA fragmentation observed in both treated and fresh semen. This is the first study investigates the influence of supplementation of date palm extract on preserved aceh cattle spermatozoa diluted in egg yolk tris based extender. |
| first_indexed | 2024-04-13T14:54:18Z |
| format | Article |
| id | doaj.art-591e30a5041a4bafa2cac8937aa74d4a |
| institution | Directory Open Access Journal |
| issn | 2085-191X 2338-7610 |
| language | English |
| last_indexed | 2024-04-13T14:54:18Z |
| publishDate | 2019-04-01 |
| publisher | Universitas Negeri Semarang |
| record_format | Article |
| series | Biosaintifika: Journal of Biology & Biology Education |
| spelling | doaj.art-591e30a5041a4bafa2cac8937aa74d4a2022-12-22T02:42:29ZengUniversitas Negeri SemarangBiosaintifika: Journal of Biology & Biology Education2085-191X2338-76102019-04-0111111712410.15294/biosaintifika.v11i1.180338502Cryopreservation of Aceh Cattle Semen with Date (Phoenix dactylifera) Extract SupplementationYonadiah Dwitya0Kartini Eriani1Hendra Saputra2Al-Azhar Al-Azhar3Muhammad Rizal4Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Syiah KualaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, IndonesiaAnimal Husbandry Office of Aceh Province, IndonesiaFaculty of Veterinary Medicine, Universitas Syiah Kuala, IndonesiaDepartment of Animal Science, Faculty of Agriculture, Universitas Lambung MangkuratCryopreservation process could affect spermatozoa quality during from reactive oxygen species (ROS) produced in cellular metabolism and the environment. Spermatozoa damage caused by ROS during cryopreservation can be reduced with the addition of natural antioxidant which commonly found in fruits like date palm. This research was done to investigate the influence of date extract on semen quality after cryopreservation. This experimental study used a completely randomized design with 4 treatments and 6 replications. Semen collected from two aceh cattle bulls was diluted in tris egg yolk extender contained different concentrations (v/v) of date extract: 0% (P0, control), 0.75% (P1), 1% (P2), and 1.25% (P3) before cryopreserved at -196 ºC for 7 days. Semen quality prior to and after cryopreservation as well as sperm DNA integrity were determined by standard microscopic and laddering methods, respectively. The results showed that the addition of 1% date extract could maintain viability (68.67%), plasma membrane integrity (62.33%), and abnormality (18.58%) of aceh cattle spermatozoa, but unable to maintain its motility above 40%. There was no DNA fragmentation observed in both treated and fresh semen. This is the first study investigates the influence of supplementation of date palm extract on preserved aceh cattle spermatozoa diluted in egg yolk tris based extender.https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/18033phoenix dactyliferacryopreservationspermatozoa qualityaceh cattle |
| spellingShingle | Yonadiah Dwitya Kartini Eriani Hendra Saputra Al-Azhar Al-Azhar Muhammad Rizal Cryopreservation of Aceh Cattle Semen with Date (Phoenix dactylifera) Extract Supplementation Biosaintifika: Journal of Biology & Biology Education phoenix dactylifera cryopreservation spermatozoa quality aceh cattle |
| title | Cryopreservation of Aceh Cattle Semen with Date (Phoenix dactylifera) Extract Supplementation |
| title_full | Cryopreservation of Aceh Cattle Semen with Date (Phoenix dactylifera) Extract Supplementation |
| title_fullStr | Cryopreservation of Aceh Cattle Semen with Date (Phoenix dactylifera) Extract Supplementation |
| title_full_unstemmed | Cryopreservation of Aceh Cattle Semen with Date (Phoenix dactylifera) Extract Supplementation |
| title_short | Cryopreservation of Aceh Cattle Semen with Date (Phoenix dactylifera) Extract Supplementation |
| title_sort | cryopreservation of aceh cattle semen with date phoenix dactylifera extract supplementation |
| topic | phoenix dactylifera cryopreservation spermatozoa quality aceh cattle |
| url | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/18033 |
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