Cellular sensitivity to UV-irradiation is mediated by RNA polymerase I transcription.
The nucleolus has long been considered to be a pure ribosome factory. However, over the last two decades it became clear that the nucleolus is involved in numerous other functions besides ribosome biogenesis. Our experiments indicate that the activity of RNA polymerase I (Pol I) transcription monito...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5479586?pdf=render |
| _version_ | 1819123657892429824 |
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| author | Robin Assfalg Marius Costel Alupei Maximilian Wagner Sylvia Koch Omar Garcia Gonzalez Adrian Schelling Karin Scharffetter-Kochanek Sebastian Iben |
| author_facet | Robin Assfalg Marius Costel Alupei Maximilian Wagner Sylvia Koch Omar Garcia Gonzalez Adrian Schelling Karin Scharffetter-Kochanek Sebastian Iben |
| author_sort | Robin Assfalg |
| collection | DOAJ |
| description | The nucleolus has long been considered to be a pure ribosome factory. However, over the last two decades it became clear that the nucleolus is involved in numerous other functions besides ribosome biogenesis. Our experiments indicate that the activity of RNA polymerase I (Pol I) transcription monitors the integrity of the DNA and influences the response to nucleolar stress as well as the rate of survival. Cells with a repressed ribosomal DNA (rDNA) transcription activity showed an increased and prolonged p53 stabilisation after UVC-irradiation. Furthermore, p53 stabilisation after inhibition and especially after UVC-irradiation might be due to abrogation of the HDM2-p53 degradation pathway by ribosomal proteins (RPs). Apoptosis mediated by highly activated p53 is a typical hallmark of Cockayne syndrome cells and transcriptional abnormalities and the following activation of the RP-HDM2-p53 pathway would be a possible explanation. |
| first_indexed | 2024-12-22T07:11:50Z |
| format | Article |
| id | doaj.art-591eb3bbe4ea4ff1a21513df0458c341 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T07:11:50Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-591eb3bbe4ea4ff1a21513df0458c3412022-12-21T18:34:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01126e017984310.1371/journal.pone.0179843Cellular sensitivity to UV-irradiation is mediated by RNA polymerase I transcription.Robin AssfalgMarius Costel AlupeiMaximilian WagnerSylvia KochOmar Garcia GonzalezAdrian SchellingKarin Scharffetter-KochanekSebastian IbenThe nucleolus has long been considered to be a pure ribosome factory. However, over the last two decades it became clear that the nucleolus is involved in numerous other functions besides ribosome biogenesis. Our experiments indicate that the activity of RNA polymerase I (Pol I) transcription monitors the integrity of the DNA and influences the response to nucleolar stress as well as the rate of survival. Cells with a repressed ribosomal DNA (rDNA) transcription activity showed an increased and prolonged p53 stabilisation after UVC-irradiation. Furthermore, p53 stabilisation after inhibition and especially after UVC-irradiation might be due to abrogation of the HDM2-p53 degradation pathway by ribosomal proteins (RPs). Apoptosis mediated by highly activated p53 is a typical hallmark of Cockayne syndrome cells and transcriptional abnormalities and the following activation of the RP-HDM2-p53 pathway would be a possible explanation.http://europepmc.org/articles/PMC5479586?pdf=render |
| spellingShingle | Robin Assfalg Marius Costel Alupei Maximilian Wagner Sylvia Koch Omar Garcia Gonzalez Adrian Schelling Karin Scharffetter-Kochanek Sebastian Iben Cellular sensitivity to UV-irradiation is mediated by RNA polymerase I transcription. PLoS ONE |
| title | Cellular sensitivity to UV-irradiation is mediated by RNA polymerase I transcription. |
| title_full | Cellular sensitivity to UV-irradiation is mediated by RNA polymerase I transcription. |
| title_fullStr | Cellular sensitivity to UV-irradiation is mediated by RNA polymerase I transcription. |
| title_full_unstemmed | Cellular sensitivity to UV-irradiation is mediated by RNA polymerase I transcription. |
| title_short | Cellular sensitivity to UV-irradiation is mediated by RNA polymerase I transcription. |
| title_sort | cellular sensitivity to uv irradiation is mediated by rna polymerase i transcription |
| url | http://europepmc.org/articles/PMC5479586?pdf=render |
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