Transcriptional regulation of proanthocyanidin biosynthesis pathway genes and transcription factors in Indigofera stachyodes Lindl. roots
Abstract Background Proanthocyanidins (PAs) have always been considered as important medicinal value component. In order to gain insights into the PA biosynthesis regulatory network in I. stachyodes roots, we analyzed the transcriptome of the I. stachyodes in Leaf, Stem, RootI (one-year-old root), a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-09-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-022-03794-4 |
| _version_ | 1811267498529521664 |
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| author | Chongmin Wang Jun Li Tao Zhou Yongping Zhang Haijun Jin Xiaoqing Liu |
| author_facet | Chongmin Wang Jun Li Tao Zhou Yongping Zhang Haijun Jin Xiaoqing Liu |
| author_sort | Chongmin Wang |
| collection | DOAJ |
| description | Abstract Background Proanthocyanidins (PAs) have always been considered as important medicinal value component. In order to gain insights into the PA biosynthesis regulatory network in I. stachyodes roots, we analyzed the transcriptome of the I. stachyodes in Leaf, Stem, RootI (one-year-old root), and RootII (two-year-old root). Results In this study, a total of 110,779 non-redundant unigenes were obtained, of which 63,863 could be functionally annotated. Simultaneously, 75 structural genes that regulate PA biosynthesis were identified, of these 6 structural genes (IsF3′H1, IsANR2, IsLAR2, IsUGT72L1-3, IsMATE2, IsMATE3) may play an important role in the synthesis of PAs in I. stachyodes roots. Furthermore, co-expression network analysis revealed that 34 IsMYBs, 18 IsbHLHs, 15 IsWRKYs, 9 IsMADSs, and 3 IsWIPs hub TFs are potential regulators for PA accumulation. Among them, IsMYB24 and IsMYB79 may be closely involved in the PA biosynthesis in I. stachyodes roots. Conclusions The biosynthesis of PAs in I. stachyodes roots is mainly produced by the subsequent pathway of cyanidin. Our work provides new insights into the molecular pathways underlying PA accumulation and enhances our global understanding of transcriptome dynamics throughout different tissues. |
| first_indexed | 2024-04-12T21:03:39Z |
| format | Article |
| id | doaj.art-592191fab1fd480692c5041504f803bc |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-12T21:03:39Z |
| publishDate | 2022-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-592191fab1fd480692c5041504f803bc2022-12-22T03:16:47ZengBMCBMC Plant Biology1471-22292022-09-0122111710.1186/s12870-022-03794-4Transcriptional regulation of proanthocyanidin biosynthesis pathway genes and transcription factors in Indigofera stachyodes Lindl. rootsChongmin Wang0Jun Li1Tao Zhou2Yongping Zhang3Haijun Jin4Xiaoqing Liu5Guizhou University of Traditional Chinese MedicineGuizhou University of Traditional Chinese MedicineGuizhou University of Traditional Chinese MedicineGuizhou University of Traditional Chinese MedicineGuizhou University of Traditional Chinese MedicineGuizhou University of Traditional Chinese MedicineAbstract Background Proanthocyanidins (PAs) have always been considered as important medicinal value component. In order to gain insights into the PA biosynthesis regulatory network in I. stachyodes roots, we analyzed the transcriptome of the I. stachyodes in Leaf, Stem, RootI (one-year-old root), and RootII (two-year-old root). Results In this study, a total of 110,779 non-redundant unigenes were obtained, of which 63,863 could be functionally annotated. Simultaneously, 75 structural genes that regulate PA biosynthesis were identified, of these 6 structural genes (IsF3′H1, IsANR2, IsLAR2, IsUGT72L1-3, IsMATE2, IsMATE3) may play an important role in the synthesis of PAs in I. stachyodes roots. Furthermore, co-expression network analysis revealed that 34 IsMYBs, 18 IsbHLHs, 15 IsWRKYs, 9 IsMADSs, and 3 IsWIPs hub TFs are potential regulators for PA accumulation. Among them, IsMYB24 and IsMYB79 may be closely involved in the PA biosynthesis in I. stachyodes roots. Conclusions The biosynthesis of PAs in I. stachyodes roots is mainly produced by the subsequent pathway of cyanidin. Our work provides new insights into the molecular pathways underlying PA accumulation and enhances our global understanding of transcriptome dynamics throughout different tissues.https://doi.org/10.1186/s12870-022-03794-4Indigofera stachyodesProanthocyanidinsBiosynthesisTranscriptomeTranscription factors |
| spellingShingle | Chongmin Wang Jun Li Tao Zhou Yongping Zhang Haijun Jin Xiaoqing Liu Transcriptional regulation of proanthocyanidin biosynthesis pathway genes and transcription factors in Indigofera stachyodes Lindl. roots BMC Plant Biology Indigofera stachyodes Proanthocyanidins Biosynthesis Transcriptome Transcription factors |
| title | Transcriptional regulation of proanthocyanidin biosynthesis pathway genes and transcription factors in Indigofera stachyodes Lindl. roots |
| title_full | Transcriptional regulation of proanthocyanidin biosynthesis pathway genes and transcription factors in Indigofera stachyodes Lindl. roots |
| title_fullStr | Transcriptional regulation of proanthocyanidin biosynthesis pathway genes and transcription factors in Indigofera stachyodes Lindl. roots |
| title_full_unstemmed | Transcriptional regulation of proanthocyanidin biosynthesis pathway genes and transcription factors in Indigofera stachyodes Lindl. roots |
| title_short | Transcriptional regulation of proanthocyanidin biosynthesis pathway genes and transcription factors in Indigofera stachyodes Lindl. roots |
| title_sort | transcriptional regulation of proanthocyanidin biosynthesis pathway genes and transcription factors in indigofera stachyodes lindl roots |
| topic | Indigofera stachyodes Proanthocyanidins Biosynthesis Transcriptome Transcription factors |
| url | https://doi.org/10.1186/s12870-022-03794-4 |
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