A real-time PCR assay for the detection of atypical strains of Chlamydiaceae from pigeons.

Recent evidence of the occurrence of atypical Chlamydiaceae strains in pigeons, different from the established Chlamydiaceae, requires the development of a specific and rapid detection tool to investigate their prevalence and significance. Here is described a new real-time PCR assay that allows spec...

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Main Authors: Aleksandar Zocevic, Fabien Vorimore, Nadia Vicari, Julien Gasparini, Lisa Jacquin, Konrad Sachse, Simone Magnino, Karine Laroucau
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3597529?pdf=render
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author Aleksandar Zocevic
Fabien Vorimore
Nadia Vicari
Julien Gasparini
Lisa Jacquin
Konrad Sachse
Simone Magnino
Karine Laroucau
author_facet Aleksandar Zocevic
Fabien Vorimore
Nadia Vicari
Julien Gasparini
Lisa Jacquin
Konrad Sachse
Simone Magnino
Karine Laroucau
author_sort Aleksandar Zocevic
collection DOAJ
description Recent evidence of the occurrence of atypical Chlamydiaceae strains in pigeons, different from the established Chlamydiaceae, requires the development of a specific and rapid detection tool to investigate their prevalence and significance. Here is described a new real-time PCR assay that allows specific detection of atypical Chlamydiaceae from pigeons. The assay has been used to assess the dissemination of these strains in field samples collected from Parisian pigeon populations in 2009. The results suggest a limited dissemination compared to the usually higher prevalence of Chlamydia psittaci that is the main species associated with avian chlamydiosis.
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spelling doaj.art-5921f51e81644ef288ab6b5d8b1eaeb12022-12-21T23:24:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0183e5874110.1371/journal.pone.0058741A real-time PCR assay for the detection of atypical strains of Chlamydiaceae from pigeons.Aleksandar ZocevicFabien VorimoreNadia VicariJulien GaspariniLisa JacquinKonrad SachseSimone MagninoKarine LaroucauRecent evidence of the occurrence of atypical Chlamydiaceae strains in pigeons, different from the established Chlamydiaceae, requires the development of a specific and rapid detection tool to investigate their prevalence and significance. Here is described a new real-time PCR assay that allows specific detection of atypical Chlamydiaceae from pigeons. The assay has been used to assess the dissemination of these strains in field samples collected from Parisian pigeon populations in 2009. The results suggest a limited dissemination compared to the usually higher prevalence of Chlamydia psittaci that is the main species associated with avian chlamydiosis.http://europepmc.org/articles/PMC3597529?pdf=render
spellingShingle Aleksandar Zocevic
Fabien Vorimore
Nadia Vicari
Julien Gasparini
Lisa Jacquin
Konrad Sachse
Simone Magnino
Karine Laroucau
A real-time PCR assay for the detection of atypical strains of Chlamydiaceae from pigeons.
PLoS ONE
title A real-time PCR assay for the detection of atypical strains of Chlamydiaceae from pigeons.
title_full A real-time PCR assay for the detection of atypical strains of Chlamydiaceae from pigeons.
title_fullStr A real-time PCR assay for the detection of atypical strains of Chlamydiaceae from pigeons.
title_full_unstemmed A real-time PCR assay for the detection of atypical strains of Chlamydiaceae from pigeons.
title_short A real-time PCR assay for the detection of atypical strains of Chlamydiaceae from pigeons.
title_sort real time pcr assay for the detection of atypical strains of chlamydiaceae from pigeons
url http://europepmc.org/articles/PMC3597529?pdf=render
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