Genome-Wide Transcript Analysis of Bradyrhizobium japonicum Bacteroids in Soybean Root Nodules
The transcriptome of endosymbiotic Bradyrhizobium japonicum bacteroids was assessed, using RNA extracted from determinate soybean root nodules. Results were compared with the transcript profiles of B. japonicum cells grown in either aerobic or microaerobic culture. Microoxia is a known trigger for t...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2007-11-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-11-1353 |
| _version_ | 1818757258240065536 |
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| author | Gabriella Pessi Christian H. Ahrens Hubert Rehrauer Andrea Lindemann Felix Hauser Hans-Martin Fischer Hauke Hennecke |
| author_facet | Gabriella Pessi Christian H. Ahrens Hubert Rehrauer Andrea Lindemann Felix Hauser Hans-Martin Fischer Hauke Hennecke |
| author_sort | Gabriella Pessi |
| collection | DOAJ |
| description | The transcriptome of endosymbiotic Bradyrhizobium japonicum bacteroids was assessed, using RNA extracted from determinate soybean root nodules. Results were compared with the transcript profiles of B. japonicum cells grown in either aerobic or microaerobic culture. Microoxia is a known trigger for the induction of symbiotically relevant genes. In fact, one third of the genes induced in bacteroids at day 21 after inoculation are congruent with those up-regulated in culture by a decreased oxygen concentration. The other induced genes, however, may be regulated by cues other than oxygen limitation. Both groups of genes provide a rich source for the possible discovery of novel functions related to symbiosis. Samples taken at different timepoints in nodule development have led to the distinction of genes expressed early and late in bacteroids. The experimental approach applied here is also useful for B. japonicum mutant analyses. As an example, we compared the transcriptome of wild-type bacteroids with that of bacteroids formed by a mutant defective in the RNA polymerase transcription factor σ54. This led to a collection of hitherto unrecognized B. japonicum genes potentially transcribed in planta in a σ54-dependent manner. |
| first_indexed | 2024-12-18T06:08:04Z |
| format | Article |
| id | doaj.art-59226fb0bc2248afba543b0be05aae97 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-18T06:08:04Z |
| publishDate | 2007-11-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-59226fb0bc2248afba543b0be05aae972022-12-21T21:18:28ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062007-11-0120111353136310.1094/MPMI-20-11-1353Genome-Wide Transcript Analysis of Bradyrhizobium japonicum Bacteroids in Soybean Root NodulesGabriella PessiChristian H. AhrensHubert RehrauerAndrea LindemannFelix HauserHans-Martin FischerHauke HenneckeThe transcriptome of endosymbiotic Bradyrhizobium japonicum bacteroids was assessed, using RNA extracted from determinate soybean root nodules. Results were compared with the transcript profiles of B. japonicum cells grown in either aerobic or microaerobic culture. Microoxia is a known trigger for the induction of symbiotically relevant genes. In fact, one third of the genes induced in bacteroids at day 21 after inoculation are congruent with those up-regulated in culture by a decreased oxygen concentration. The other induced genes, however, may be regulated by cues other than oxygen limitation. Both groups of genes provide a rich source for the possible discovery of novel functions related to symbiosis. Samples taken at different timepoints in nodule development have led to the distinction of genes expressed early and late in bacteroids. The experimental approach applied here is also useful for B. japonicum mutant analyses. As an example, we compared the transcriptome of wild-type bacteroids with that of bacteroids formed by a mutant defective in the RNA polymerase transcription factor σ54. This led to a collection of hitherto unrecognized B. japonicum genes potentially transcribed in planta in a σ54-dependent manner.https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-11-1353GeneChipnitrogen fixationRpoN |
| spellingShingle | Gabriella Pessi Christian H. Ahrens Hubert Rehrauer Andrea Lindemann Felix Hauser Hans-Martin Fischer Hauke Hennecke Genome-Wide Transcript Analysis of Bradyrhizobium japonicum Bacteroids in Soybean Root Nodules Molecular Plant-Microbe Interactions GeneChip nitrogen fixation RpoN |
| title | Genome-Wide Transcript Analysis of Bradyrhizobium japonicum Bacteroids in Soybean Root Nodules |
| title_full | Genome-Wide Transcript Analysis of Bradyrhizobium japonicum Bacteroids in Soybean Root Nodules |
| title_fullStr | Genome-Wide Transcript Analysis of Bradyrhizobium japonicum Bacteroids in Soybean Root Nodules |
| title_full_unstemmed | Genome-Wide Transcript Analysis of Bradyrhizobium japonicum Bacteroids in Soybean Root Nodules |
| title_short | Genome-Wide Transcript Analysis of Bradyrhizobium japonicum Bacteroids in Soybean Root Nodules |
| title_sort | genome wide transcript analysis of bradyrhizobium japonicum bacteroids in soybean root nodules |
| topic | GeneChip nitrogen fixation RpoN |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-11-1353 |
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