RAGE-Dependent Effect of Exogenous Methylglyoxal Intake on Lung Biomechanics in Mice
Methylglyoxal (MG) is a known highly reactive dicarbonyl and precursor to free radicals and advanced glycation end-products (AGEs). It is discussed to be involved in tissue aging and in the pathogenesis of different degenerative diseases. The effect of long-term oral administration of MG, simulating...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/15/1/23 |
| _version_ | 1797431346480545792 |
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| author | Samiya Al-Robaiy Alexander Navarrete Santos Andreas Simm |
| author_facet | Samiya Al-Robaiy Alexander Navarrete Santos Andreas Simm |
| author_sort | Samiya Al-Robaiy |
| collection | DOAJ |
| description | Methylglyoxal (MG) is a known highly reactive dicarbonyl and precursor to free radicals and advanced glycation end-products (AGEs). It is discussed to be involved in tissue aging and in the pathogenesis of different degenerative diseases. The effect of long-term oral administration of MG, simulating dietary MG intake, on the lung biomechanics of wild type (WT) and receptor for advanced glycation end-products knockout (RAGE-KO) mice was studied using an ex vivo ventilation system starting at the age of 6 months and after feeding for 6 and 12 months with MG. Our results showed that MG was taken up in the circulation and efficiently excreted with urine. The amount of free urinary MG measured after 12 months of feeding was lowered. After 12 months feeding, a significant airway resistance increase accompanied by a decrease of the maximal inspiratory airflow was observed in WT animals. No effect of MG in lung function of RAGE-KO mice could be detected. Despite the evidence that MG entered the systemic circulation, no MG-derived AGE accumulation was detected in the lung lysates in dependency on MG-feeding. Our data indicate that the short-term feeding of MG has little effect in vivo. Only after long-term treatment was MG secretion reduced, leading to tissue impairment. |
| first_indexed | 2024-03-09T09:43:34Z |
| format | Article |
| id | doaj.art-5925a4adc9404e3caa29a99ae239da4d |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T09:43:34Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-5925a4adc9404e3caa29a99ae239da4d2023-12-02T00:45:27ZengMDPI AGNutrients2072-66432022-12-011512310.3390/nu15010023RAGE-Dependent Effect of Exogenous Methylglyoxal Intake on Lung Biomechanics in MiceSamiya Al-Robaiy0Alexander Navarrete Santos1Andreas Simm2Center for Basic Medical Research (ZMG), University Hospital Halle (Saale), Martin Luther University Halle-Wittenberg, 06108 Halle (Saale), GermanyCenter for Basic Medical Research (ZMG), University Hospital Halle (Saale), Martin Luther University Halle-Wittenberg, 06108 Halle (Saale), GermanyCenter for Basic Medical Research (ZMG), University Hospital Halle (Saale), Martin Luther University Halle-Wittenberg, 06108 Halle (Saale), GermanyMethylglyoxal (MG) is a known highly reactive dicarbonyl and precursor to free radicals and advanced glycation end-products (AGEs). It is discussed to be involved in tissue aging and in the pathogenesis of different degenerative diseases. The effect of long-term oral administration of MG, simulating dietary MG intake, on the lung biomechanics of wild type (WT) and receptor for advanced glycation end-products knockout (RAGE-KO) mice was studied using an ex vivo ventilation system starting at the age of 6 months and after feeding for 6 and 12 months with MG. Our results showed that MG was taken up in the circulation and efficiently excreted with urine. The amount of free urinary MG measured after 12 months of feeding was lowered. After 12 months feeding, a significant airway resistance increase accompanied by a decrease of the maximal inspiratory airflow was observed in WT animals. No effect of MG in lung function of RAGE-KO mice could be detected. Despite the evidence that MG entered the systemic circulation, no MG-derived AGE accumulation was detected in the lung lysates in dependency on MG-feeding. Our data indicate that the short-term feeding of MG has little effect in vivo. Only after long-term treatment was MG secretion reduced, leading to tissue impairment.https://www.mdpi.com/2072-6643/15/1/23methylglyoxallung biomechanicsreceptor for advanced glycation end-productsex vivo ventilation |
| spellingShingle | Samiya Al-Robaiy Alexander Navarrete Santos Andreas Simm RAGE-Dependent Effect of Exogenous Methylglyoxal Intake on Lung Biomechanics in Mice Nutrients methylglyoxal lung biomechanics receptor for advanced glycation end-products ex vivo ventilation |
| title | RAGE-Dependent Effect of Exogenous Methylglyoxal Intake on Lung Biomechanics in Mice |
| title_full | RAGE-Dependent Effect of Exogenous Methylglyoxal Intake on Lung Biomechanics in Mice |
| title_fullStr | RAGE-Dependent Effect of Exogenous Methylglyoxal Intake on Lung Biomechanics in Mice |
| title_full_unstemmed | RAGE-Dependent Effect of Exogenous Methylglyoxal Intake on Lung Biomechanics in Mice |
| title_short | RAGE-Dependent Effect of Exogenous Methylglyoxal Intake on Lung Biomechanics in Mice |
| title_sort | rage dependent effect of exogenous methylglyoxal intake on lung biomechanics in mice |
| topic | methylglyoxal lung biomechanics receptor for advanced glycation end-products ex vivo ventilation |
| url | https://www.mdpi.com/2072-6643/15/1/23 |
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