Histidine Promotes the Glucose Synthesis through Activation of the Gluconeogenic Pathway in Bovine Hepatocytes
Histidine (His) is considered to be the first-limiting amino acid (AA) on grass silage-based diets in lactation cows, which correlate positively with lactose yield. The higher glucose requirements of lactating cows can be met through a combination of increased capacity for gluconeogenesis and increa...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/11/3295 |
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| author | Tianyu Yang Zhiqiang Cheng Maocheng Jiang Xiaoyu Ma Osmond Datsomor Guoqi Zhao Kang Zhan |
| author_facet | Tianyu Yang Zhiqiang Cheng Maocheng Jiang Xiaoyu Ma Osmond Datsomor Guoqi Zhao Kang Zhan |
| author_sort | Tianyu Yang |
| collection | DOAJ |
| description | Histidine (His) is considered to be the first-limiting amino acid (AA) on grass silage-based diets in lactation cows, which correlate positively with lactose yield. The higher glucose requirements of lactating cows can be met through a combination of increased capacity for gluconeogenesis and increased supply of gluconeogenic precursors. However, the effect of His on the expression of gluconeogenic genes in the bovine hepatocytes is less known. Therefore, this study aimed to investigate the regulatory effect of His on the key gluconeogenic genes and glucose output in bovine hepatocytes. The addition of 0.15, 0.6, and 1.2 mM His in a medium significantly enhanced (<i>p</i> < 0.05) the viability of bovine hepatocytes. Remarkably, 1.2 mM His induced profound changes (<i>p</i> < 0.05) in the mRNA level of key genes involved in gluconeogenesis, including PCK1, PCK2, FBP1, and G6PC in vitro. Furthermore, the mRNA expression of PCK1 was significantly elevated (<i>p</i> < 0.05) by the addition of 1.2 mM His at 3, 6, 12, and 24 h of incubation. The hepatic glucose output increased (<i>p</i> < 0.05) linearly with increasing His concentration. These findings indicate that the addition of His may be efficiently converted into glucose via the upregulation of genes related to the gluconeogenic pathway. |
| first_indexed | 2024-03-10T05:46:04Z |
| format | Article |
| id | doaj.art-59264fed9775401a854e6c408d941ec5 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T05:46:04Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-59264fed9775401a854e6c408d941ec52023-11-22T22:08:28ZengMDPI AGAnimals2076-26152021-11-011111329510.3390/ani11113295Histidine Promotes the Glucose Synthesis through Activation of the Gluconeogenic Pathway in Bovine HepatocytesTianyu Yang0Zhiqiang Cheng1Maocheng Jiang2Xiaoyu Ma3Osmond Datsomor4Guoqi Zhao5Kang Zhan6College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaHistidine (His) is considered to be the first-limiting amino acid (AA) on grass silage-based diets in lactation cows, which correlate positively with lactose yield. The higher glucose requirements of lactating cows can be met through a combination of increased capacity for gluconeogenesis and increased supply of gluconeogenic precursors. However, the effect of His on the expression of gluconeogenic genes in the bovine hepatocytes is less known. Therefore, this study aimed to investigate the regulatory effect of His on the key gluconeogenic genes and glucose output in bovine hepatocytes. The addition of 0.15, 0.6, and 1.2 mM His in a medium significantly enhanced (<i>p</i> < 0.05) the viability of bovine hepatocytes. Remarkably, 1.2 mM His induced profound changes (<i>p</i> < 0.05) in the mRNA level of key genes involved in gluconeogenesis, including PCK1, PCK2, FBP1, and G6PC in vitro. Furthermore, the mRNA expression of PCK1 was significantly elevated (<i>p</i> < 0.05) by the addition of 1.2 mM His at 3, 6, 12, and 24 h of incubation. The hepatic glucose output increased (<i>p</i> < 0.05) linearly with increasing His concentration. These findings indicate that the addition of His may be efficiently converted into glucose via the upregulation of genes related to the gluconeogenic pathway.https://www.mdpi.com/2076-2615/11/11/3295histidinebovine hepatocytesgluconeogenesis |
| spellingShingle | Tianyu Yang Zhiqiang Cheng Maocheng Jiang Xiaoyu Ma Osmond Datsomor Guoqi Zhao Kang Zhan Histidine Promotes the Glucose Synthesis through Activation of the Gluconeogenic Pathway in Bovine Hepatocytes Animals histidine bovine hepatocytes gluconeogenesis |
| title | Histidine Promotes the Glucose Synthesis through Activation of the Gluconeogenic Pathway in Bovine Hepatocytes |
| title_full | Histidine Promotes the Glucose Synthesis through Activation of the Gluconeogenic Pathway in Bovine Hepatocytes |
| title_fullStr | Histidine Promotes the Glucose Synthesis through Activation of the Gluconeogenic Pathway in Bovine Hepatocytes |
| title_full_unstemmed | Histidine Promotes the Glucose Synthesis through Activation of the Gluconeogenic Pathway in Bovine Hepatocytes |
| title_short | Histidine Promotes the Glucose Synthesis through Activation of the Gluconeogenic Pathway in Bovine Hepatocytes |
| title_sort | histidine promotes the glucose synthesis through activation of the gluconeogenic pathway in bovine hepatocytes |
| topic | histidine bovine hepatocytes gluconeogenesis |
| url | https://www.mdpi.com/2076-2615/11/11/3295 |
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