A meta-barcoding census of freshwater planktonic protists in Appalachia – Natural Tunnel State Park, Virginia, USA
The purpose of our study was to survey the freshwater planktonic protists within an inland natural preserve in the Ridge and Valley physiographic province of the Appalachian Region using metabarcoding. Microbial eukaryotes are essential primary producers and predators in small freshwater ecosystems,...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2018-10-01
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| Series: | Metabarcoding and Metagenomics |
| Online Access: | https://mbmg.pensoft.net/article/26939/ |
| _version_ | 1830205757066313728 |
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| author | A. Bruce Cahoon Ashley G. Huffman Megan M. Krager Roseanna M. Crowell |
| author_facet | A. Bruce Cahoon Ashley G. Huffman Megan M. Krager Roseanna M. Crowell |
| author_sort | A. Bruce Cahoon |
| collection | DOAJ |
| description | The purpose of our study was to survey the freshwater planktonic protists within an inland natural preserve in the Ridge and Valley physiographic province of the Appalachian Region using metabarcoding. Microbial eukaryotes are essential primary producers and predators in small freshwater ecosystems, yet they are often overlooked due to the difficulty of identification. This has been remedied, in part, by the cost reduction of high throughput DNA sequencing and the growth of barcode databases, making the identification and analysis of microorganisms by way of metabarcoding surveys in complex ecosystems increasingly feasible. Water samples were collected from five sites at the Natural Tunnel State Park in Scott County, VA (USA), representing three common bodies of water found in this region. Samples were initially collected during a Bioblitz event in April 2016 and then seven and fourteen weeks afterwards. Metabarcode analysis of the 23S and 18S genes identified 3663 OTUs representing 213 family level and 332 genus level taxa. This study provides an initial barcode census within a region that has a reputation as a temperate biodiversity “hotspot”. The overall protist diversity was comparably high to other temperate systems, but not unusually high; the microalgal diversity, however, was higher than that reported for other temperate regions. The three types of water bodies had their own distinctive protist biomes despite close proximity. |
| first_indexed | 2024-12-18T03:59:19Z |
| format | Article |
| id | doaj.art-592831cc48e74c0d8fa0cef2001255b0 |
| institution | Directory Open Access Journal |
| issn | 2534-9708 |
| language | English |
| last_indexed | 2024-12-18T03:59:19Z |
| publishDate | 2018-10-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Metabarcoding and Metagenomics |
| spelling | doaj.art-592831cc48e74c0d8fa0cef2001255b02022-12-21T21:21:43ZengPensoft PublishersMetabarcoding and Metagenomics2534-97082018-10-01211210.3897/mbmg.2.2693926939A meta-barcoding census of freshwater planktonic protists in Appalachia – Natural Tunnel State Park, Virginia, USAA. Bruce Cahoon0Ashley G. Huffman1Megan M. Krager2Roseanna M. Crowell3University of Virginia’s College at WiseUniversity of Virginia’s College at WiseBays Mountain ParkUniversity of Virginia’s College at WiseThe purpose of our study was to survey the freshwater planktonic protists within an inland natural preserve in the Ridge and Valley physiographic province of the Appalachian Region using metabarcoding. Microbial eukaryotes are essential primary producers and predators in small freshwater ecosystems, yet they are often overlooked due to the difficulty of identification. This has been remedied, in part, by the cost reduction of high throughput DNA sequencing and the growth of barcode databases, making the identification and analysis of microorganisms by way of metabarcoding surveys in complex ecosystems increasingly feasible. Water samples were collected from five sites at the Natural Tunnel State Park in Scott County, VA (USA), representing three common bodies of water found in this region. Samples were initially collected during a Bioblitz event in April 2016 and then seven and fourteen weeks afterwards. Metabarcode analysis of the 23S and 18S genes identified 3663 OTUs representing 213 family level and 332 genus level taxa. This study provides an initial barcode census within a region that has a reputation as a temperate biodiversity “hotspot”. The overall protist diversity was comparably high to other temperate systems, but not unusually high; the microalgal diversity, however, was higher than that reported for other temperate regions. The three types of water bodies had their own distinctive protist biomes despite close proximity.https://mbmg.pensoft.net/article/26939/ |
| spellingShingle | A. Bruce Cahoon Ashley G. Huffman Megan M. Krager Roseanna M. Crowell A meta-barcoding census of freshwater planktonic protists in Appalachia – Natural Tunnel State Park, Virginia, USA Metabarcoding and Metagenomics |
| title | A meta-barcoding census of freshwater planktonic protists in Appalachia – Natural Tunnel State Park, Virginia, USA |
| title_full | A meta-barcoding census of freshwater planktonic protists in Appalachia – Natural Tunnel State Park, Virginia, USA |
| title_fullStr | A meta-barcoding census of freshwater planktonic protists in Appalachia – Natural Tunnel State Park, Virginia, USA |
| title_full_unstemmed | A meta-barcoding census of freshwater planktonic protists in Appalachia – Natural Tunnel State Park, Virginia, USA |
| title_short | A meta-barcoding census of freshwater planktonic protists in Appalachia – Natural Tunnel State Park, Virginia, USA |
| title_sort | meta barcoding census of freshwater planktonic protists in appalachia natural tunnel state park virginia usa |
| url | https://mbmg.pensoft.net/article/26939/ |
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