Genome-wide characterization of the CPA gene family in potato and a preliminary functional analysis of its role in NaCl tolerance
Abstract Background The cation/proton antiporter (CPA) superfamily plays a crucial role in regulating ion homeostasis and pH in plant cells, contributing to stress resistance. However, in potato (Solanum tuberosum L.), systematic identification and analysis of CPA genes are lacking. Results A total...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-02-01
|
| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-024-10000-2 |
| _version_ | 1797275752254668800 |
|---|---|
| author | Jintao Liu Dianjue Li Jing Wang Qian Wang Xiao Guo Qi Fu Philip Kear Guangtao Zhu Xiaohui Yang |
| author_facet | Jintao Liu Dianjue Li Jing Wang Qian Wang Xiao Guo Qi Fu Philip Kear Guangtao Zhu Xiaohui Yang |
| author_sort | Jintao Liu |
| collection | DOAJ |
| description | Abstract Background The cation/proton antiporter (CPA) superfamily plays a crucial role in regulating ion homeostasis and pH in plant cells, contributing to stress resistance. However, in potato (Solanum tuberosum L.), systematic identification and analysis of CPA genes are lacking. Results A total of 33 StCPA members were identified and classified into StNHX (n = 7), StKEA (n = 6), and StCHX (n = 20) subfamilies. StCHX owned the highest number of conserved motifs, followed by StKEA and StNHX. The StNHX and StKEA subfamilies owned more exons than StCHX. NaCl stress induced the differentially expression of 19 genes in roots or leaves, among which StCHX14 and StCHX16 were specifically induced in leaves, while StCHX2 and StCHX19 were specifically expressed in the roots. A total of 11 strongly responded genes were further verified by qPCR. Six CPA family members, StNHX1, StNHX2, StNHX3, StNHX5, StNHX6 and StCHX19, were proved to transport Na+ through yeast complementation experiments. Conclusions This study provides comprehensive insights into StCPAs and their response to NaCl stress, facilitating further functional characterization. |
| first_indexed | 2024-03-07T15:18:00Z |
| format | Article |
| id | doaj.art-592af31fc7c44cf38a389b0817be45ea |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-03-07T15:18:00Z |
| publishDate | 2024-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-592af31fc7c44cf38a389b0817be45ea2024-03-05T17:47:31ZengBMCBMC Genomics1471-21642024-02-0125111310.1186/s12864-024-10000-2Genome-wide characterization of the CPA gene family in potato and a preliminary functional analysis of its role in NaCl toleranceJintao Liu0Dianjue Li1Jing Wang2Qian Wang3Xiao Guo4Qi Fu5Philip Kear6Guangtao Zhu7Xiaohui Yang8Key Lab for Potato Biology in Universities of Yunnan, School of Life Sciences, Yunnan Normal UniversityKey Lab for Potato Biology in Universities of Yunnan, School of Life Sciences, Yunnan Normal UniversityKey Lab for Potato Biology in Universities of Yunnan, School of Life Sciences, Yunnan Normal UniversityKey Lab for Potato Biology in Universities of Yunnan, School of Life Sciences, Yunnan Normal UniversityInstitute of Vegetables, Shandong Academy of Agricultural Sciences/Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Sub-CenterKey Lab for Potato Biology in Universities of Yunnan, School of Life Sciences, Yunnan Normal UniversityInternational Potato Center (CIP), CIP China Center for Asia PacificKey Lab for Potato Biology in Universities of Yunnan, School of Life Sciences, Yunnan Normal UniversityInstitute of Vegetables, Shandong Academy of Agricultural Sciences/Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Sub-CenterAbstract Background The cation/proton antiporter (CPA) superfamily plays a crucial role in regulating ion homeostasis and pH in plant cells, contributing to stress resistance. However, in potato (Solanum tuberosum L.), systematic identification and analysis of CPA genes are lacking. Results A total of 33 StCPA members were identified and classified into StNHX (n = 7), StKEA (n = 6), and StCHX (n = 20) subfamilies. StCHX owned the highest number of conserved motifs, followed by StKEA and StNHX. The StNHX and StKEA subfamilies owned more exons than StCHX. NaCl stress induced the differentially expression of 19 genes in roots or leaves, among which StCHX14 and StCHX16 were specifically induced in leaves, while StCHX2 and StCHX19 were specifically expressed in the roots. A total of 11 strongly responded genes were further verified by qPCR. Six CPA family members, StNHX1, StNHX2, StNHX3, StNHX5, StNHX6 and StCHX19, were proved to transport Na+ through yeast complementation experiments. Conclusions This study provides comprehensive insights into StCPAs and their response to NaCl stress, facilitating further functional characterization.https://doi.org/10.1186/s12864-024-10000-2PotatoCPANaCl stressGene expressionIon transporter |
| spellingShingle | Jintao Liu Dianjue Li Jing Wang Qian Wang Xiao Guo Qi Fu Philip Kear Guangtao Zhu Xiaohui Yang Genome-wide characterization of the CPA gene family in potato and a preliminary functional analysis of its role in NaCl tolerance BMC Genomics Potato CPA NaCl stress Gene expression Ion transporter |
| title | Genome-wide characterization of the CPA gene family in potato and a preliminary functional analysis of its role in NaCl tolerance |
| title_full | Genome-wide characterization of the CPA gene family in potato and a preliminary functional analysis of its role in NaCl tolerance |
| title_fullStr | Genome-wide characterization of the CPA gene family in potato and a preliminary functional analysis of its role in NaCl tolerance |
| title_full_unstemmed | Genome-wide characterization of the CPA gene family in potato and a preliminary functional analysis of its role in NaCl tolerance |
| title_short | Genome-wide characterization of the CPA gene family in potato and a preliminary functional analysis of its role in NaCl tolerance |
| title_sort | genome wide characterization of the cpa gene family in potato and a preliminary functional analysis of its role in nacl tolerance |
| topic | Potato CPA NaCl stress Gene expression Ion transporter |
| url | https://doi.org/10.1186/s12864-024-10000-2 |
| work_keys_str_mv | AT jintaoliu genomewidecharacterizationofthecpagenefamilyinpotatoandapreliminaryfunctionalanalysisofitsroleinnacltolerance AT dianjueli genomewidecharacterizationofthecpagenefamilyinpotatoandapreliminaryfunctionalanalysisofitsroleinnacltolerance AT jingwang genomewidecharacterizationofthecpagenefamilyinpotatoandapreliminaryfunctionalanalysisofitsroleinnacltolerance AT qianwang genomewidecharacterizationofthecpagenefamilyinpotatoandapreliminaryfunctionalanalysisofitsroleinnacltolerance AT xiaoguo genomewidecharacterizationofthecpagenefamilyinpotatoandapreliminaryfunctionalanalysisofitsroleinnacltolerance AT qifu genomewidecharacterizationofthecpagenefamilyinpotatoandapreliminaryfunctionalanalysisofitsroleinnacltolerance AT philipkear genomewidecharacterizationofthecpagenefamilyinpotatoandapreliminaryfunctionalanalysisofitsroleinnacltolerance AT guangtaozhu genomewidecharacterizationofthecpagenefamilyinpotatoandapreliminaryfunctionalanalysisofitsroleinnacltolerance AT xiaohuiyang genomewidecharacterizationofthecpagenefamilyinpotatoandapreliminaryfunctionalanalysisofitsroleinnacltolerance |