Peanut butter feeding induces oral tolerance in genetically diverse collaborative cross mice
BackgroundEarly dietary introduction of peanut has shown efficacy in clinical trials and driven pediatric recommendations for early introduction of peanut to children with heightened allergy risk worldwide. Unfortunately, tolerance is not induced in every case, and a subset of patients are allergic...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-07-01
|
| Series: | Frontiers in Allergy |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/falgy.2023.1219268/full |
| _version_ | 1797778308986830848 |
|---|---|
| author | Robert M. Immormino Johanna M. Smeekens Johanna M. Smeekens Priscilla I. Mathai Janelle R. Kesselring Janelle R. Kesselring Andrew V. Turner Andrew V. Turner Michael D. Kulis Michael D. Kulis Timothy P. Moran |
| author_facet | Robert M. Immormino Johanna M. Smeekens Johanna M. Smeekens Priscilla I. Mathai Janelle R. Kesselring Janelle R. Kesselring Andrew V. Turner Andrew V. Turner Michael D. Kulis Michael D. Kulis Timothy P. Moran |
| author_sort | Robert M. Immormino |
| collection | DOAJ |
| description | BackgroundEarly dietary introduction of peanut has shown efficacy in clinical trials and driven pediatric recommendations for early introduction of peanut to children with heightened allergy risk worldwide. Unfortunately, tolerance is not induced in every case, and a subset of patients are allergic prior to introduction. Here we assess peanut allergic sensitization and oral tolerance in genetically diverse mouse strains.ObjectiveWe aimed to determine whether environmental adjuvant-driven airway sensitization and oral tolerance to peanut could be induced in various genetically diverse mouse strains.MethodsC57BL/6J and 12 Collaborative Cross (CC) mouse strains were fed regular chow or ad libitum peanut butter to induce tolerance. Tolerance was tested by attempting to sensitize mice via intratracheal exposure to peanut and lipopolysaccharide (LPS), followed by intraperitoneal peanut challenge. Peanut-specific immunoglobulins and peanut-induced anaphylaxis were assessed.ResultsWithout oral peanut feeding, most CC strains (11/12) and C57BL/6J induced peanut-specific IgE and IgG1 following airway exposure to peanut and LPS. With oral peanut feeding none of the CC strains nor C57BL/6J mice became sensitized to peanut or experienced anaphylaxis following peanut challenge.ConclusionAllergic sensitization and oral tolerance to peanut can be achieved across a range of genetically diverse mice. Notably, the same strains that became allergic via airway sensitization were tolerized by feeding high doses of peanut butter before sensitization, suggesting that the order and route of peanut exposure are critical for determining the allergic fate. |
| first_indexed | 2024-03-12T23:15:21Z |
| format | Article |
| id | doaj.art-592b64addfd6418d96db6ce758376f7e |
| institution | Directory Open Access Journal |
| issn | 2673-6101 |
| language | English |
| last_indexed | 2024-03-12T23:15:21Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Allergy |
| spelling | doaj.art-592b64addfd6418d96db6ce758376f7e2023-07-17T09:45:12ZengFrontiers Media S.A.Frontiers in Allergy2673-61012023-07-01410.3389/falgy.2023.12192681219268Peanut butter feeding induces oral tolerance in genetically diverse collaborative cross miceRobert M. Immormino0Johanna M. Smeekens1Johanna M. Smeekens2Priscilla I. Mathai3Janelle R. Kesselring4Janelle R. Kesselring5Andrew V. Turner6Andrew V. Turner7Michael D. Kulis8Michael D. Kulis9Timothy P. Moran10Department of Pediatrics, UNC School of Medicine, Chapel Hill, NC, United StatesDepartment of Pediatrics, UNC School of Medicine, Chapel Hill, NC, United StatesUNC Food Allergy Initiative, Department of Pediatrics, UNC School of Medicine, Chapel Hill, NC, United StatesDepartment of Pediatrics, UNC School of Medicine, Chapel Hill, NC, United StatesDepartment of Pediatrics, UNC School of Medicine, Chapel Hill, NC, United StatesUNC Food Allergy Initiative, Department of Pediatrics, UNC School of Medicine, Chapel Hill, NC, United StatesDepartment of Pediatrics, UNC School of Medicine, Chapel Hill, NC, United StatesUNC Food Allergy Initiative, Department of Pediatrics, UNC School of Medicine, Chapel Hill, NC, United StatesDepartment of Pediatrics, UNC School of Medicine, Chapel Hill, NC, United StatesUNC Food Allergy Initiative, Department of Pediatrics, UNC School of Medicine, Chapel Hill, NC, United StatesDepartment of Pediatrics, UNC School of Medicine, Chapel Hill, NC, United StatesBackgroundEarly dietary introduction of peanut has shown efficacy in clinical trials and driven pediatric recommendations for early introduction of peanut to children with heightened allergy risk worldwide. Unfortunately, tolerance is not induced in every case, and a subset of patients are allergic prior to introduction. Here we assess peanut allergic sensitization and oral tolerance in genetically diverse mouse strains.ObjectiveWe aimed to determine whether environmental adjuvant-driven airway sensitization and oral tolerance to peanut could be induced in various genetically diverse mouse strains.MethodsC57BL/6J and 12 Collaborative Cross (CC) mouse strains were fed regular chow or ad libitum peanut butter to induce tolerance. Tolerance was tested by attempting to sensitize mice via intratracheal exposure to peanut and lipopolysaccharide (LPS), followed by intraperitoneal peanut challenge. Peanut-specific immunoglobulins and peanut-induced anaphylaxis were assessed.ResultsWithout oral peanut feeding, most CC strains (11/12) and C57BL/6J induced peanut-specific IgE and IgG1 following airway exposure to peanut and LPS. With oral peanut feeding none of the CC strains nor C57BL/6J mice became sensitized to peanut or experienced anaphylaxis following peanut challenge.ConclusionAllergic sensitization and oral tolerance to peanut can be achieved across a range of genetically diverse mice. Notably, the same strains that became allergic via airway sensitization were tolerized by feeding high doses of peanut butter before sensitization, suggesting that the order and route of peanut exposure are critical for determining the allergic fate.https://www.frontiersin.org/articles/10.3389/falgy.2023.1219268/fullfood allergypeanut allergyoral tolerancecollaborative crossairway sensitization |
| spellingShingle | Robert M. Immormino Johanna M. Smeekens Johanna M. Smeekens Priscilla I. Mathai Janelle R. Kesselring Janelle R. Kesselring Andrew V. Turner Andrew V. Turner Michael D. Kulis Michael D. Kulis Timothy P. Moran Peanut butter feeding induces oral tolerance in genetically diverse collaborative cross mice Frontiers in Allergy food allergy peanut allergy oral tolerance collaborative cross airway sensitization |
| title | Peanut butter feeding induces oral tolerance in genetically diverse collaborative cross mice |
| title_full | Peanut butter feeding induces oral tolerance in genetically diverse collaborative cross mice |
| title_fullStr | Peanut butter feeding induces oral tolerance in genetically diverse collaborative cross mice |
| title_full_unstemmed | Peanut butter feeding induces oral tolerance in genetically diverse collaborative cross mice |
| title_short | Peanut butter feeding induces oral tolerance in genetically diverse collaborative cross mice |
| title_sort | peanut butter feeding induces oral tolerance in genetically diverse collaborative cross mice |
| topic | food allergy peanut allergy oral tolerance collaborative cross airway sensitization |
| url | https://www.frontiersin.org/articles/10.3389/falgy.2023.1219268/full |
| work_keys_str_mv | AT robertmimmormino peanutbutterfeedinginducesoraltoleranceingeneticallydiversecollaborativecrossmice AT johannamsmeekens peanutbutterfeedinginducesoraltoleranceingeneticallydiversecollaborativecrossmice AT johannamsmeekens peanutbutterfeedinginducesoraltoleranceingeneticallydiversecollaborativecrossmice AT priscillaimathai peanutbutterfeedinginducesoraltoleranceingeneticallydiversecollaborativecrossmice AT janellerkesselring peanutbutterfeedinginducesoraltoleranceingeneticallydiversecollaborativecrossmice AT janellerkesselring peanutbutterfeedinginducesoraltoleranceingeneticallydiversecollaborativecrossmice AT andrewvturner peanutbutterfeedinginducesoraltoleranceingeneticallydiversecollaborativecrossmice AT andrewvturner peanutbutterfeedinginducesoraltoleranceingeneticallydiversecollaborativecrossmice AT michaeldkulis peanutbutterfeedinginducesoraltoleranceingeneticallydiversecollaborativecrossmice AT michaeldkulis peanutbutterfeedinginducesoraltoleranceingeneticallydiversecollaborativecrossmice AT timothypmoran peanutbutterfeedinginducesoraltoleranceingeneticallydiversecollaborativecrossmice |