Control of actin polymerization via reactive oxygen species generation using light or radiation
Actin is one of the most prevalent proteins in cells, and its amino acid sequence is remarkably conserved from protozoa to humans. The polymerization-depolymerization cycle of actin immediately below the plasma membrane regulates cell function, motility, and morphology. It is known that actin and ot...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2022.1014008/full |
| _version_ | 1818058289488855040 |
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| author | Tetsuya Ishimoto Tetsuya Ishimoto Hisashi Mori Hisashi Mori Hisashi Mori |
| author_facet | Tetsuya Ishimoto Tetsuya Ishimoto Hisashi Mori Hisashi Mori Hisashi Mori |
| author_sort | Tetsuya Ishimoto |
| collection | DOAJ |
| description | Actin is one of the most prevalent proteins in cells, and its amino acid sequence is remarkably conserved from protozoa to humans. The polymerization-depolymerization cycle of actin immediately below the plasma membrane regulates cell function, motility, and morphology. It is known that actin and other actin-binding proteins are targets for reactive oxygen species (ROS), indicating that ROS affects cells through actin reorganization. Several researchers have attempted to control actin polymerization from outside the cell to mimic or inhibit actin reorganization. To modify the polymerization state of actin, ultraviolet, visible, and near-infrared light, ionizing radiation, and chromophore-assisted light inactivation have all been reported to induce ROS. Additionally, a combination of the fluorescent protein KillerRed and the luminescent protein luciferase can generate ROS on actin fibers and promote actin polymerization. These techniques are very useful tools for analyzing the relationship between ROS and cell function, movement, and morphology, and are also expected to be used in therapeutics. In this mini review, we offer an overview of the advancements in this field, with a particular focus on how to control intracellular actin polymerization using such optical approaches, and discuss future challenges. |
| first_indexed | 2024-12-10T12:58:16Z |
| format | Article |
| id | doaj.art-592b989dcb6549729cbc1eb4d624930a |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-10T12:58:16Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-592b989dcb6549729cbc1eb4d624930a2022-12-22T01:48:01ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-09-011010.3389/fcell.2022.10140081014008Control of actin polymerization via reactive oxygen species generation using light or radiationTetsuya Ishimoto0Tetsuya Ishimoto1Hisashi Mori2Hisashi Mori3Hisashi Mori4Department of Molecular Neuroscience, Faculty of Medicine, University of Toyama, Toyama, JapanResearch Center for Idling Brain Science, University of Toyama, Toyama, JapanDepartment of Molecular Neuroscience, Faculty of Medicine, University of Toyama, Toyama, JapanResearch Center for Idling Brain Science, University of Toyama, Toyama, JapanResearch Center for Pre-Disease Science, University of Toyama, Toyama, JapanActin is one of the most prevalent proteins in cells, and its amino acid sequence is remarkably conserved from protozoa to humans. The polymerization-depolymerization cycle of actin immediately below the plasma membrane regulates cell function, motility, and morphology. It is known that actin and other actin-binding proteins are targets for reactive oxygen species (ROS), indicating that ROS affects cells through actin reorganization. Several researchers have attempted to control actin polymerization from outside the cell to mimic or inhibit actin reorganization. To modify the polymerization state of actin, ultraviolet, visible, and near-infrared light, ionizing radiation, and chromophore-assisted light inactivation have all been reported to induce ROS. Additionally, a combination of the fluorescent protein KillerRed and the luminescent protein luciferase can generate ROS on actin fibers and promote actin polymerization. These techniques are very useful tools for analyzing the relationship between ROS and cell function, movement, and morphology, and are also expected to be used in therapeutics. In this mini review, we offer an overview of the advancements in this field, with a particular focus on how to control intracellular actin polymerization using such optical approaches, and discuss future challenges.https://www.frontiersin.org/articles/10.3389/fcell.2022.1014008/fullactinreactive oxygen specieslaserultravioletpolymerizationchromophore-assisted light inactivation |
| spellingShingle | Tetsuya Ishimoto Tetsuya Ishimoto Hisashi Mori Hisashi Mori Hisashi Mori Control of actin polymerization via reactive oxygen species generation using light or radiation Frontiers in Cell and Developmental Biology actin reactive oxygen species laser ultraviolet polymerization chromophore-assisted light inactivation |
| title | Control of actin polymerization via reactive oxygen species generation using light or radiation |
| title_full | Control of actin polymerization via reactive oxygen species generation using light or radiation |
| title_fullStr | Control of actin polymerization via reactive oxygen species generation using light or radiation |
| title_full_unstemmed | Control of actin polymerization via reactive oxygen species generation using light or radiation |
| title_short | Control of actin polymerization via reactive oxygen species generation using light or radiation |
| title_sort | control of actin polymerization via reactive oxygen species generation using light or radiation |
| topic | actin reactive oxygen species laser ultraviolet polymerization chromophore-assisted light inactivation |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2022.1014008/full |
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