Luteolin-Fabricated ZnO Nanostructures Showed PLK-1 Mediated Anti-Breast Cancer Activity
The present work describes a facile and convenient procedure for synthesizing zinc oxide nanoparticles using luteolin isolated from <i>Eclipta alba</i> plant (L-ZnONPs) at room temperature. The formation of as-grown L-ZnONPs was confirmed by X-ray diffraction analysis (XRD), scanning ele...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/3/385 |
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| author | Shiva Prasad Kollur Shashanka K. Prasad Sushma Pradeep Ravindra Veerapur Sharanagouda S. Patil Raghavendra G. Amachawadi Rajendra Prasad S Ghada Lamraoui Abdulaziz A. Al-Kheraif Abdallah M. Elgorban Asad Syed Chandan Shivamallu |
| author_facet | Shiva Prasad Kollur Shashanka K. Prasad Sushma Pradeep Ravindra Veerapur Sharanagouda S. Patil Raghavendra G. Amachawadi Rajendra Prasad S Ghada Lamraoui Abdulaziz A. Al-Kheraif Abdallah M. Elgorban Asad Syed Chandan Shivamallu |
| author_sort | Shiva Prasad Kollur |
| collection | DOAJ |
| description | The present work describes a facile and convenient procedure for synthesizing zinc oxide nanoparticles using luteolin isolated from <i>Eclipta alba</i> plant (L-ZnONPs) at room temperature. The formation of as-grown L-ZnONPs was confirmed by X-ray diffraction analysis (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HR-TEM), and selected area electron diffraction (SAED). The Wurtzite structure of ZnO was observed by its hexagonal phases in diffraction patterns. The SEM images revealed the different sizes and morphologies of L-ZnONPs, with diameters between 12 and 25 nm. The HR-TEM result showed that the inter-planar distance between two lattice fringes was 0.262 nm, which coincides with the d-spacing of (002) and (101) lattice planes of the as-obtained material. The anticancer activity of L-ZnONPs against the breast cancer cell line MCF-7 was greater as compared to that of luteolin or ZnO alone. The mechanistic evaluation of such an activity carried out using in silico methods suggested that the anti-breast cancer activity of L-ZnONPs was mediated by polo-like kinase 1 (PLK1) proteins. |
| first_indexed | 2024-03-09T05:22:06Z |
| format | Article |
| id | doaj.art-592dd56c27bb4361b3b01a80c2489746 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T05:22:06Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-592dd56c27bb4361b3b01a80c24897462023-12-03T12:39:43ZengMDPI AGBiomolecules2218-273X2021-03-0111338510.3390/biom11030385Luteolin-Fabricated ZnO Nanostructures Showed PLK-1 Mediated Anti-Breast Cancer ActivityShiva Prasad Kollur0Shashanka K. Prasad1Sushma Pradeep2Ravindra Veerapur3Sharanagouda S. Patil4Raghavendra G. Amachawadi5Rajendra Prasad S6Ghada Lamraoui7Abdulaziz A. Al-Kheraif8Abdallah M. Elgorban9Asad Syed10Chandan Shivamallu11Department of Sciences, Amrita School of Arts and Sciences, Amrita Vishwa Vidyapeetham, Mysuru Campus, Mysuru, Karnataka 570 026, IndiaDepartment of Biotechnology and Bioinformatics, School of Life Sciences, JSS Academy of Higher Education and Research, Mysuru, Karnataka 570 015, IndiaDepartment of Biotechnology and Bioinformatics, School of Life Sciences, JSS Academy of Higher Education and Research, Mysuru, Karnataka 570 015, IndiaDepartment of Metallurgy and Materials Engineering, Malawi Institute of Technology, Malawi University of Science and Technology, P.O. Box 5916, Limbe 312229, MalawiICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka 560 064, IndiaDepartment of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506-5606, USADepartment of Chemistry, Davangere University, Shivagangotri, Davangere, Karnataka 577 007, IndiaNature and Life Sciences, Earth and Universe Sciences, University of Tlemcen, Tlemcen 13000, AlgeriaDental Biomaterials Research Chair, Dental Health Department, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Biotechnology and Bioinformatics, School of Life Sciences, JSS Academy of Higher Education and Research, Mysuru, Karnataka 570 015, IndiaThe present work describes a facile and convenient procedure for synthesizing zinc oxide nanoparticles using luteolin isolated from <i>Eclipta alba</i> plant (L-ZnONPs) at room temperature. The formation of as-grown L-ZnONPs was confirmed by X-ray diffraction analysis (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HR-TEM), and selected area electron diffraction (SAED). The Wurtzite structure of ZnO was observed by its hexagonal phases in diffraction patterns. The SEM images revealed the different sizes and morphologies of L-ZnONPs, with diameters between 12 and 25 nm. The HR-TEM result showed that the inter-planar distance between two lattice fringes was 0.262 nm, which coincides with the d-spacing of (002) and (101) lattice planes of the as-obtained material. The anticancer activity of L-ZnONPs against the breast cancer cell line MCF-7 was greater as compared to that of luteolin or ZnO alone. The mechanistic evaluation of such an activity carried out using in silico methods suggested that the anti-breast cancer activity of L-ZnONPs was mediated by polo-like kinase 1 (PLK1) proteins.https://www.mdpi.com/2218-273X/11/3/385luteolinZnONPsin silico analysisPLK1 proteins |
| spellingShingle | Shiva Prasad Kollur Shashanka K. Prasad Sushma Pradeep Ravindra Veerapur Sharanagouda S. Patil Raghavendra G. Amachawadi Rajendra Prasad S Ghada Lamraoui Abdulaziz A. Al-Kheraif Abdallah M. Elgorban Asad Syed Chandan Shivamallu Luteolin-Fabricated ZnO Nanostructures Showed PLK-1 Mediated Anti-Breast Cancer Activity Biomolecules luteolin ZnONPs in silico analysis PLK1 proteins |
| title | Luteolin-Fabricated ZnO Nanostructures Showed PLK-1 Mediated Anti-Breast Cancer Activity |
| title_full | Luteolin-Fabricated ZnO Nanostructures Showed PLK-1 Mediated Anti-Breast Cancer Activity |
| title_fullStr | Luteolin-Fabricated ZnO Nanostructures Showed PLK-1 Mediated Anti-Breast Cancer Activity |
| title_full_unstemmed | Luteolin-Fabricated ZnO Nanostructures Showed PLK-1 Mediated Anti-Breast Cancer Activity |
| title_short | Luteolin-Fabricated ZnO Nanostructures Showed PLK-1 Mediated Anti-Breast Cancer Activity |
| title_sort | luteolin fabricated zno nanostructures showed plk 1 mediated anti breast cancer activity |
| topic | luteolin ZnONPs in silico analysis PLK1 proteins |
| url | https://www.mdpi.com/2218-273X/11/3/385 |
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