Identification of SL addition trans-splicing acceptor sites in the internal transcribed spacer I region of pre-rRNA in Leishmania (Leishmania) amazonensis
Trypanosomatidae is a family of early branching eukaryotes harbouring a distinctive repertoire of gene expression strategies. Functional mature messenger RNA is generated via the trans-splicing and polyadenylation processing of constitutively transcribed polycistronic units. Recently, trans-splicing...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2012-12-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000800019&lng=en&tlng=en |
| _version_ | 1797703040838402048 |
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| author | Mario Gustavo Mayer Lucile Maria Floeter-Winter |
| author_facet | Mario Gustavo Mayer Lucile Maria Floeter-Winter |
| author_sort | Mario Gustavo Mayer |
| collection | DOAJ |
| description | Trypanosomatidae is a family of early branching eukaryotes harbouring a distinctive repertoire of gene expression strategies. Functional mature messenger RNA is generated via the trans-splicing and polyadenylation processing of constitutively transcribed polycistronic units. Recently, trans-splicing of pre-small subunit ribosomal RNA in the 5' external transcribed spacer region and of precursor tRNAsec have been described. Here, we used a previously validated semi-nested reverse transcription-polymerase chain reaction strategy to investigate internal transcribed spacer (ITS) I acceptor sites in total RNA from Leishmania (Leishmania) amazonensis. Two distinct spliced leader-containing RNAs were detected indicating that trans-splicing reactions occur at two AG acceptor sites mapped in this ITS region. These data provide further evidence of the wide spectrum of RNA molecules that act as trans-splicing acceptors in trypanosomatids. |
| first_indexed | 2024-03-12T04:58:39Z |
| format | Article |
| id | doaj.art-592f36b36d3c4513a776585401d301f3 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T04:58:39Z |
| publishDate | 2012-12-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-592f36b36d3c4513a776585401d301f32023-09-03T09:14:29ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602012-12-0110781070107210.1590/S0074-02762012000800019S0074-02762012000800019Identification of SL addition trans-splicing acceptor sites in the internal transcribed spacer I region of pre-rRNA in Leishmania (Leishmania) amazonensisMario Gustavo Mayer0Lucile Maria Floeter-Winter1Instituto ButantanUniversidade de São PauloTrypanosomatidae is a family of early branching eukaryotes harbouring a distinctive repertoire of gene expression strategies. Functional mature messenger RNA is generated via the trans-splicing and polyadenylation processing of constitutively transcribed polycistronic units. Recently, trans-splicing of pre-small subunit ribosomal RNA in the 5' external transcribed spacer region and of precursor tRNAsec have been described. Here, we used a previously validated semi-nested reverse transcription-polymerase chain reaction strategy to investigate internal transcribed spacer (ITS) I acceptor sites in total RNA from Leishmania (Leishmania) amazonensis. Two distinct spliced leader-containing RNAs were detected indicating that trans-splicing reactions occur at two AG acceptor sites mapped in this ITS region. These data provide further evidence of the wide spectrum of RNA molecules that act as trans-splicing acceptors in trypanosomatids.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000800019&lng=en&tlng=enRNA polymerase Ispliced leader RNAspliceosome |
| spellingShingle | Mario Gustavo Mayer Lucile Maria Floeter-Winter Identification of SL addition trans-splicing acceptor sites in the internal transcribed spacer I region of pre-rRNA in Leishmania (Leishmania) amazonensis Memorias do Instituto Oswaldo Cruz RNA polymerase I spliced leader RNA spliceosome |
| title | Identification of SL addition trans-splicing acceptor sites in the internal transcribed spacer I region of pre-rRNA in Leishmania (Leishmania) amazonensis |
| title_full | Identification of SL addition trans-splicing acceptor sites in the internal transcribed spacer I region of pre-rRNA in Leishmania (Leishmania) amazonensis |
| title_fullStr | Identification of SL addition trans-splicing acceptor sites in the internal transcribed spacer I region of pre-rRNA in Leishmania (Leishmania) amazonensis |
| title_full_unstemmed | Identification of SL addition trans-splicing acceptor sites in the internal transcribed spacer I region of pre-rRNA in Leishmania (Leishmania) amazonensis |
| title_short | Identification of SL addition trans-splicing acceptor sites in the internal transcribed spacer I region of pre-rRNA in Leishmania (Leishmania) amazonensis |
| title_sort | identification of sl addition trans splicing acceptor sites in the internal transcribed spacer i region of pre rrna in leishmania leishmania amazonensis |
| topic | RNA polymerase I spliced leader RNA spliceosome |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000800019&lng=en&tlng=en |
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