Development of DNA-based species-specific real-time PCR markers for four berry fruits and their application in commercial berry fruit foods
Abstract Berry fruits have attracted attention because of their purported benefits for aging, cardiovascular disease, and cancer. Therefore, highly priced berry fruits might be targets for food adulteration and fraud. In this study, eight species-specific primer sets based on the single nucleotide p...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2019-03-01
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| Series: | Applied Biological Chemistry |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13765-019-0413-9 |
| _version_ | 1818354630774489088 |
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| author | Jun An Jun-Cheol Moon Ju Hee Kim Geum Sol Kim Cheol Seong Jang |
| author_facet | Jun An Jun-Cheol Moon Ju Hee Kim Geum Sol Kim Cheol Seong Jang |
| author_sort | Jun An |
| collection | DOAJ |
| description | Abstract Berry fruits have attracted attention because of their purported benefits for aging, cardiovascular disease, and cancer. Therefore, highly priced berry fruits might be targets for food adulteration and fraud. In this study, eight species-specific primer sets based on the single nucleotide polymorphism of the chloroplast genomes of four berry fruits (aronia, blackberry, cranberry, and strawberry) were developed for quantitative real-time PCR (qPCR) analysis by SYBR Green staining with the aim of preventing berry fruit food fraud. The developed primer pairs exhibited high efficiencies ranging from 88 to 110% with strong correlation coefficients (R2 > 0.99) for the amplification of each target species. However, no clear correlation coefficients were evident for non-target species. To evaluate the practicality of the developed primers, 18 commercial berry fruit products were verified by qPCR analysis. The developed primer pairs were amplified to a low Ct value (range 16.1–23.3) for the target species and proved capable of detecting target species in berry fruit commercial foods. Therefore, the developed qPCR-based species-specific markers could be suitable for the prevention of berry fruit food fraud and to verify marker reliability. |
| first_indexed | 2024-12-13T19:28:29Z |
| format | Article |
| id | doaj.art-592f764fb8a64a2f853b714ad78a912f |
| institution | Directory Open Access Journal |
| issn | 2468-0834 2468-0842 |
| language | English |
| last_indexed | 2024-12-13T19:28:29Z |
| publishDate | 2019-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Applied Biological Chemistry |
| spelling | doaj.art-592f764fb8a64a2f853b714ad78a912f2022-12-21T23:33:59ZengSpringerOpenApplied Biological Chemistry2468-08342468-08422019-03-016211710.1186/s13765-019-0413-9Development of DNA-based species-specific real-time PCR markers for four berry fruits and their application in commercial berry fruit foodsJun An0Jun-Cheol Moon1Ju Hee Kim2Geum Sol Kim3Cheol Seong Jang4Plant Genomics Laboratory, Department of Bio-Resources Sciences, Kangwon National UniversityAgriculture and Life Sciences Research Institute, Kangwon National UniversityPlant Genomics Laboratory, Department of Bio-Resources Sciences, Kangwon National UniversityPlant Genomics Laboratory, Department of Bio-Resources Sciences, Kangwon National UniversityPlant Genomics Laboratory, Department of Bio-Resources Sciences, Kangwon National UniversityAbstract Berry fruits have attracted attention because of their purported benefits for aging, cardiovascular disease, and cancer. Therefore, highly priced berry fruits might be targets for food adulteration and fraud. In this study, eight species-specific primer sets based on the single nucleotide polymorphism of the chloroplast genomes of four berry fruits (aronia, blackberry, cranberry, and strawberry) were developed for quantitative real-time PCR (qPCR) analysis by SYBR Green staining with the aim of preventing berry fruit food fraud. The developed primer pairs exhibited high efficiencies ranging from 88 to 110% with strong correlation coefficients (R2 > 0.99) for the amplification of each target species. However, no clear correlation coefficients were evident for non-target species. To evaluate the practicality of the developed primers, 18 commercial berry fruit products were verified by qPCR analysis. The developed primer pairs were amplified to a low Ct value (range 16.1–23.3) for the target species and proved capable of detecting target species in berry fruit commercial foods. Therefore, the developed qPCR-based species-specific markers could be suitable for the prevention of berry fruit food fraud and to verify marker reliability.http://link.springer.com/article/10.1186/s13765-019-0413-9BerryFood fraud preventionReal-time PCRSpecies-specific DNA markersSYBR Green |
| spellingShingle | Jun An Jun-Cheol Moon Ju Hee Kim Geum Sol Kim Cheol Seong Jang Development of DNA-based species-specific real-time PCR markers for four berry fruits and their application in commercial berry fruit foods Applied Biological Chemistry Berry Food fraud prevention Real-time PCR Species-specific DNA markers SYBR Green |
| title | Development of DNA-based species-specific real-time PCR markers for four berry fruits and their application in commercial berry fruit foods |
| title_full | Development of DNA-based species-specific real-time PCR markers for four berry fruits and their application in commercial berry fruit foods |
| title_fullStr | Development of DNA-based species-specific real-time PCR markers for four berry fruits and their application in commercial berry fruit foods |
| title_full_unstemmed | Development of DNA-based species-specific real-time PCR markers for four berry fruits and their application in commercial berry fruit foods |
| title_short | Development of DNA-based species-specific real-time PCR markers for four berry fruits and their application in commercial berry fruit foods |
| title_sort | development of dna based species specific real time pcr markers for four berry fruits and their application in commercial berry fruit foods |
| topic | Berry Food fraud prevention Real-time PCR Species-specific DNA markers SYBR Green |
| url | http://link.springer.com/article/10.1186/s13765-019-0413-9 |
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