Cytoplasmic anion/cation imbalances applied across the membrane capacitance may form a significant component of the resting membrane potential of red blood cells
Abstract Electrical aspects of cell function manifest in many ways. The most widely studied is the cell membrane potential, V m , but others include the conductance and capacitance of the membrane, the conductance of the enclosed cytoplasm, as well as the charge at the cell surface (an electrical do...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-19316-z |
| _version_ | 1828143960309104640 |
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| author | Michael Pycraft Hughes Christopher H. Fry Fatima H. Labeed |
| author_facet | Michael Pycraft Hughes Christopher H. Fry Fatima H. Labeed |
| author_sort | Michael Pycraft Hughes |
| collection | DOAJ |
| description | Abstract Electrical aspects of cell function manifest in many ways. The most widely studied is the cell membrane potential, V m , but others include the conductance and capacitance of the membrane, the conductance of the enclosed cytoplasm, as well as the charge at the cell surface (an electrical double layer) producing an extracellular electrical potential, the ζ-potential. Empirical relationships have been identified between many of these, but not the mechanisms that link them all. Here we examine relationships between V m and the electrical conductivities of both the cytoplasm and extracellular media, using data from a suspensions of red blood cells. We have identified linear relationships between extracellular medium conductivity, cytoplasm conductivity and V m . This is in contrast to the standard model of a resting membrane potential which describes a logarithmic relationship between V m and the concentration of permeable ions in the extracellular medium. The model here suggests that V m is partially electrostatic in origin, arising from a charge imbalance at an inner electrical double-layer, acting across the membrane and double-layer capacitances to produce a voltage. This model describes an origin for coupling between V m and ζ, by which cells can alter their electrostatic relationship with their environment, with implications for modulation of membrane ion transport, adhesion of proteins such as antibodies and wider cell–cell interactions. |
| first_indexed | 2024-04-11T20:08:00Z |
| format | Article |
| id | doaj.art-5930e4a115244b8bb54ac97e4bf8acd7 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T20:08:00Z |
| publishDate | 2022-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-5930e4a115244b8bb54ac97e4bf8acd72022-12-22T04:05:16ZengNature PortfolioScientific Reports2045-23222022-09-0112111010.1038/s41598-022-19316-zCytoplasmic anion/cation imbalances applied across the membrane capacitance may form a significant component of the resting membrane potential of red blood cellsMichael Pycraft Hughes0Christopher H. Fry1Fatima H. Labeed2Department of Biomedical Engineering, Khalifa UniversitySchool of Physiology, Pharmacology and Neuroscience, University of BristolCentre for Biomedical Engineering, University of SurreyAbstract Electrical aspects of cell function manifest in many ways. The most widely studied is the cell membrane potential, V m , but others include the conductance and capacitance of the membrane, the conductance of the enclosed cytoplasm, as well as the charge at the cell surface (an electrical double layer) producing an extracellular electrical potential, the ζ-potential. Empirical relationships have been identified between many of these, but not the mechanisms that link them all. Here we examine relationships between V m and the electrical conductivities of both the cytoplasm and extracellular media, using data from a suspensions of red blood cells. We have identified linear relationships between extracellular medium conductivity, cytoplasm conductivity and V m . This is in contrast to the standard model of a resting membrane potential which describes a logarithmic relationship between V m and the concentration of permeable ions in the extracellular medium. The model here suggests that V m is partially electrostatic in origin, arising from a charge imbalance at an inner electrical double-layer, acting across the membrane and double-layer capacitances to produce a voltage. This model describes an origin for coupling between V m and ζ, by which cells can alter their electrostatic relationship with their environment, with implications for modulation of membrane ion transport, adhesion of proteins such as antibodies and wider cell–cell interactions.https://doi.org/10.1038/s41598-022-19316-z |
| spellingShingle | Michael Pycraft Hughes Christopher H. Fry Fatima H. Labeed Cytoplasmic anion/cation imbalances applied across the membrane capacitance may form a significant component of the resting membrane potential of red blood cells Scientific Reports |
| title | Cytoplasmic anion/cation imbalances applied across the membrane capacitance may form a significant component of the resting membrane potential of red blood cells |
| title_full | Cytoplasmic anion/cation imbalances applied across the membrane capacitance may form a significant component of the resting membrane potential of red blood cells |
| title_fullStr | Cytoplasmic anion/cation imbalances applied across the membrane capacitance may form a significant component of the resting membrane potential of red blood cells |
| title_full_unstemmed | Cytoplasmic anion/cation imbalances applied across the membrane capacitance may form a significant component of the resting membrane potential of red blood cells |
| title_short | Cytoplasmic anion/cation imbalances applied across the membrane capacitance may form a significant component of the resting membrane potential of red blood cells |
| title_sort | cytoplasmic anion cation imbalances applied across the membrane capacitance may form a significant component of the resting membrane potential of red blood cells |
| url | https://doi.org/10.1038/s41598-022-19316-z |
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