The Antimicrobial Efficacy of Topically Applied Mafenide Acetate, Citric Acid and Wound Irrigation Solutions Lavanox and Prontosan against <i>Pseudomonas aeruginosa</i>
Since burn wound infections caused by <i>Pseudomonas aeruginosa</i> (PA) lead to major complications and sepsis, this study evaluates the antimicrobial efficacy of the wound irrigation solutions Prontosan (PRT), Lavanox (LAV), citric acid (CA) and mafenide acetate (MA) using microbiology...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-01-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/13/1/42 |
| _version_ | 1827372863145902080 |
|---|---|
| author | Mahsa Bagheri Andreas Zoric Maria von Kohout Paul C. Fuchs Jennifer Lynn. Schiefer Christian Opländer |
| author_facet | Mahsa Bagheri Andreas Zoric Maria von Kohout Paul C. Fuchs Jennifer Lynn. Schiefer Christian Opländer |
| author_sort | Mahsa Bagheri |
| collection | DOAJ |
| description | Since burn wound infections caused by <i>Pseudomonas aeruginosa</i> (PA) lead to major complications and sepsis, this study evaluates the antimicrobial efficacy of the wound irrigation solutions Prontosan (PRT), Lavanox (LAV), citric acid (CA) and mafenide acetate (MA) using microbiology assays and an ex vivo skin wound model. In suspension assays, all the solutions showed significant reductions in bacterial number (log<sub>10</sub> reduction: CA 5.77; LAV 4.91; PRT 4.74; MA 1.23). The biofilm assay revealed that PRT and LAV reduced biofilm formation by ~25% after a 15 min treatment, while PRT was most effective after a 24 h treatment (~68%). The number of PA in biofilms measured directly after a 15 min treatment was reduced most effectively with CA and LAV (log<sub>10</sub> reductions ~2.5), whereas after a 24 h treatment, all solutions achieved only 1.36–1.65 log<sub>10</sub> reductions. In the skin wound model, PRT and LAV provided the highest bacterial reduction after a 15 min treatment (log<sub>10</sub> reduction 1.8–1.9), while MA was more effective after a 22 h treatment (log<sub>10</sub> reduction 3.6). The results demonstrated the antimicrobial efficacy of all solutions against PA. Further investigation is needed to explore the potential clinical applications of a combination or alternating use of these solutions for infection prophylaxis and treatment of wound infections caused by PA. |
| first_indexed | 2024-03-08T11:07:11Z |
| format | Article |
| id | doaj.art-5930fc167027451183734eb90f0ef9eb |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-08T11:07:11Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-5930fc167027451183734eb90f0ef9eb2024-01-26T14:37:03ZengMDPI AGAntibiotics2079-63822024-01-011314210.3390/antibiotics13010042The Antimicrobial Efficacy of Topically Applied Mafenide Acetate, Citric Acid and Wound Irrigation Solutions Lavanox and Prontosan against <i>Pseudomonas aeruginosa</i>Mahsa Bagheri0Andreas Zoric1Maria von Kohout2Paul C. Fuchs3Jennifer Lynn. Schiefer4Christian Opländer5Department of Plastic and Aesthetic Surgery, Hand Surgery, HELIOS Hospital Emil von Behring, Walterhoeferstr. 11, 14165 Berlin, GermanyPlastic, Reconstructive and Aesthetic Surgery, RKH Hospital Bietigheim-Vaihingen, Riedstr. 12, 74321 Bietigheim-Bissingen, GermanyPlastic Surgery, Hand Surgery, Burn Center, Cologne-Merheim Hospital, Witten/Herdecke University, Ostmerheimer Str. 200, 51109 Cologne, GermanyPlastic Surgery, Hand Surgery, Burn Center, Cologne-Merheim Hospital, Witten/Herdecke University, Ostmerheimer Str. 200, 51109 Cologne, GermanyPlastic Surgery, Hand Surgery, Burn Center, Cologne-Merheim Hospital, Witten/Herdecke University, Ostmerheimer Str. 200, 51109 Cologne, GermanyInstitute for Research in Operative Medicine (IFOM), Cologne-Merheim Medical Center, Witten/Herdecke University, Ostmerheimer Str. 200, 51109 Cologne, GermanySince burn wound infections caused by <i>Pseudomonas aeruginosa</i> (PA) lead to major complications and sepsis, this study evaluates the antimicrobial efficacy of the wound irrigation solutions Prontosan (PRT), Lavanox (LAV), citric acid (CA) and mafenide acetate (MA) using microbiology assays and an ex vivo skin wound model. In suspension assays, all the solutions showed significant reductions in bacterial number (log<sub>10</sub> reduction: CA 5.77; LAV 4.91; PRT 4.74; MA 1.23). The biofilm assay revealed that PRT and LAV reduced biofilm formation by ~25% after a 15 min treatment, while PRT was most effective after a 24 h treatment (~68%). The number of PA in biofilms measured directly after a 15 min treatment was reduced most effectively with CA and LAV (log<sub>10</sub> reductions ~2.5), whereas after a 24 h treatment, all solutions achieved only 1.36–1.65 log<sub>10</sub> reductions. In the skin wound model, PRT and LAV provided the highest bacterial reduction after a 15 min treatment (log<sub>10</sub> reduction 1.8–1.9), while MA was more effective after a 22 h treatment (log<sub>10</sub> reduction 3.6). The results demonstrated the antimicrobial efficacy of all solutions against PA. Further investigation is needed to explore the potential clinical applications of a combination or alternating use of these solutions for infection prophylaxis and treatment of wound infections caused by PA.https://www.mdpi.com/2079-6382/13/1/42burns<i>Pseudomonas aeruginosa</i>wound irrigation solutionsantimicrobial efficacyex vivo human skin wound model |
| spellingShingle | Mahsa Bagheri Andreas Zoric Maria von Kohout Paul C. Fuchs Jennifer Lynn. Schiefer Christian Opländer The Antimicrobial Efficacy of Topically Applied Mafenide Acetate, Citric Acid and Wound Irrigation Solutions Lavanox and Prontosan against <i>Pseudomonas aeruginosa</i> Antibiotics burns <i>Pseudomonas aeruginosa</i> wound irrigation solutions antimicrobial efficacy ex vivo human skin wound model |
| title | The Antimicrobial Efficacy of Topically Applied Mafenide Acetate, Citric Acid and Wound Irrigation Solutions Lavanox and Prontosan against <i>Pseudomonas aeruginosa</i> |
| title_full | The Antimicrobial Efficacy of Topically Applied Mafenide Acetate, Citric Acid and Wound Irrigation Solutions Lavanox and Prontosan against <i>Pseudomonas aeruginosa</i> |
| title_fullStr | The Antimicrobial Efficacy of Topically Applied Mafenide Acetate, Citric Acid and Wound Irrigation Solutions Lavanox and Prontosan against <i>Pseudomonas aeruginosa</i> |
| title_full_unstemmed | The Antimicrobial Efficacy of Topically Applied Mafenide Acetate, Citric Acid and Wound Irrigation Solutions Lavanox and Prontosan against <i>Pseudomonas aeruginosa</i> |
| title_short | The Antimicrobial Efficacy of Topically Applied Mafenide Acetate, Citric Acid and Wound Irrigation Solutions Lavanox and Prontosan against <i>Pseudomonas aeruginosa</i> |
| title_sort | antimicrobial efficacy of topically applied mafenide acetate citric acid and wound irrigation solutions lavanox and prontosan against i pseudomonas aeruginosa i |
| topic | burns <i>Pseudomonas aeruginosa</i> wound irrigation solutions antimicrobial efficacy ex vivo human skin wound model |
| url | https://www.mdpi.com/2079-6382/13/1/42 |
| work_keys_str_mv | AT mahsabagheri theantimicrobialefficacyoftopicallyappliedmafenideacetatecitricacidandwoundirrigationsolutionslavanoxandprontosanagainstipseudomonasaeruginosai AT andreaszoric theantimicrobialefficacyoftopicallyappliedmafenideacetatecitricacidandwoundirrigationsolutionslavanoxandprontosanagainstipseudomonasaeruginosai AT mariavonkohout theantimicrobialefficacyoftopicallyappliedmafenideacetatecitricacidandwoundirrigationsolutionslavanoxandprontosanagainstipseudomonasaeruginosai AT paulcfuchs theantimicrobialefficacyoftopicallyappliedmafenideacetatecitricacidandwoundirrigationsolutionslavanoxandprontosanagainstipseudomonasaeruginosai AT jenniferlynnschiefer theantimicrobialefficacyoftopicallyappliedmafenideacetatecitricacidandwoundirrigationsolutionslavanoxandprontosanagainstipseudomonasaeruginosai AT christianoplander theantimicrobialefficacyoftopicallyappliedmafenideacetatecitricacidandwoundirrigationsolutionslavanoxandprontosanagainstipseudomonasaeruginosai AT mahsabagheri antimicrobialefficacyoftopicallyappliedmafenideacetatecitricacidandwoundirrigationsolutionslavanoxandprontosanagainstipseudomonasaeruginosai AT andreaszoric antimicrobialefficacyoftopicallyappliedmafenideacetatecitricacidandwoundirrigationsolutionslavanoxandprontosanagainstipseudomonasaeruginosai AT mariavonkohout antimicrobialefficacyoftopicallyappliedmafenideacetatecitricacidandwoundirrigationsolutionslavanoxandprontosanagainstipseudomonasaeruginosai AT paulcfuchs antimicrobialefficacyoftopicallyappliedmafenideacetatecitricacidandwoundirrigationsolutionslavanoxandprontosanagainstipseudomonasaeruginosai AT jenniferlynnschiefer antimicrobialefficacyoftopicallyappliedmafenideacetatecitricacidandwoundirrigationsolutionslavanoxandprontosanagainstipseudomonasaeruginosai AT christianoplander antimicrobialefficacyoftopicallyappliedmafenideacetatecitricacidandwoundirrigationsolutionslavanoxandprontosanagainstipseudomonasaeruginosai |