Use of Three Air Samplers for the Detection of PRRSV-1 under Experimental and Field Conditions
Airborne transmission of porcine reproductive and respiratory syndrome virus (PRRSV) has been known for a long time. Most experiments were performed using PRRSV-2 strains and fairly little information is available on the airborne spread of PRRSV-1. The aim of this study was to assess three different...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-12-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/8/12/233 |
| _version_ | 1818693908107886592 |
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| author | Heiko Stein Jochen Schulz Rebecca Morgenstern Thomas Voglmayr Georg Freymüller Leonie Sinn Till Rümenapf Isabel Hennig-Pauka Andrea Ladinig |
| author_facet | Heiko Stein Jochen Schulz Rebecca Morgenstern Thomas Voglmayr Georg Freymüller Leonie Sinn Till Rümenapf Isabel Hennig-Pauka Andrea Ladinig |
| author_sort | Heiko Stein |
| collection | DOAJ |
| description | Airborne transmission of porcine reproductive and respiratory syndrome virus (PRRSV) has been known for a long time. Most experiments were performed using PRRSV-2 strains and fairly little information is available on the airborne spread of PRRSV-1. The aim of this study was to assess three different air samplers for their ability to detect PRRSV-1 under experimental and field conditions. All three devices were able to detect PRRSV-1 by quantitative reverse trascription polymerase chain reaction (qRT-PCR) under experimental conditions. However, the detection of PRRSV-1 in a PRRSV-positive farm with active virus circulation was not successful. |
| first_indexed | 2024-12-17T13:21:09Z |
| format | Article |
| id | doaj.art-59319e0f20aa4405b33cf0e2a7828e97 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-12-17T13:21:09Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-59319e0f20aa4405b33cf0e2a7828e972022-12-21T21:46:52ZengMDPI AGAnimals2076-26152018-12-0181223310.3390/ani8120233ani8120233Use of Three Air Samplers for the Detection of PRRSV-1 under Experimental and Field ConditionsHeiko Stein0Jochen Schulz1Rebecca Morgenstern2Thomas Voglmayr3Georg Freymüller4Leonie Sinn5Till Rümenapf6Isabel Hennig-Pauka7Andrea Ladinig8Department for Farm Animals and Veterinary Public Health, University Clinic for Swine, University of Veterinary Medicine Vienna, 1210 Vienna, AustriaInstitute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, GermanyBoehringer Ingelheim RCV GmbH & Co KG, 1120 Vienna, AustriaTraunkreis Vet Clinic, 4551 Ried im Traunkreis, AustriaTraunkreis Vet Clinic, 4551 Ried im Traunkreis, AustriaInstitute of Virology, Department of Pathobiology, University of Veterinary Medicine Vienna, 1210 Vienna, AustriaInstitute of Virology, Department of Pathobiology, University of Veterinary Medicine Vienna, 1210 Vienna, AustriaField Station for Epidemiology, University of Veterinary Medicine Hannover, Foundation, 49456 Bakum, GermanyDepartment for Farm Animals and Veterinary Public Health, University Clinic for Swine, University of Veterinary Medicine Vienna, 1210 Vienna, AustriaAirborne transmission of porcine reproductive and respiratory syndrome virus (PRRSV) has been known for a long time. Most experiments were performed using PRRSV-2 strains and fairly little information is available on the airborne spread of PRRSV-1. The aim of this study was to assess three different air samplers for their ability to detect PRRSV-1 under experimental and field conditions. All three devices were able to detect PRRSV-1 by quantitative reverse trascription polymerase chain reaction (qRT-PCR) under experimental conditions. However, the detection of PRRSV-1 in a PRRSV-positive farm with active virus circulation was not successful.https://www.mdpi.com/2076-2615/8/12/233PRRSVairborneair sampleraerosol |
| spellingShingle | Heiko Stein Jochen Schulz Rebecca Morgenstern Thomas Voglmayr Georg Freymüller Leonie Sinn Till Rümenapf Isabel Hennig-Pauka Andrea Ladinig Use of Three Air Samplers for the Detection of PRRSV-1 under Experimental and Field Conditions Animals PRRSV airborne air sampler aerosol |
| title | Use of Three Air Samplers for the Detection of PRRSV-1 under Experimental and Field Conditions |
| title_full | Use of Three Air Samplers for the Detection of PRRSV-1 under Experimental and Field Conditions |
| title_fullStr | Use of Three Air Samplers for the Detection of PRRSV-1 under Experimental and Field Conditions |
| title_full_unstemmed | Use of Three Air Samplers for the Detection of PRRSV-1 under Experimental and Field Conditions |
| title_short | Use of Three Air Samplers for the Detection of PRRSV-1 under Experimental and Field Conditions |
| title_sort | use of three air samplers for the detection of prrsv 1 under experimental and field conditions |
| topic | PRRSV airborne air sampler aerosol |
| url | https://www.mdpi.com/2076-2615/8/12/233 |
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