Preparation and Preliminary Evaluation of Silver-Modified Anodic Alumina for Biomedical Applications
The present study reports a specific method for preparation of silver-modified anodic alumina substrates intended for biomaterial applications. Al<sub>2</sub>O<sub>3</sub> coatings were obtained by anodization of technically pure aluminum alloy in sulfuric acid electrolyte. S...
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| Format: | Article |
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MDPI AG
2021-12-01
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| Series: | Metals |
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| Online Access: | https://www.mdpi.com/2075-4701/12/1/51 |
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| author | Denitsa Kiradzhiyska Nikolina Milcheva Rositsa Mancheva Tsvetelina Batsalova Balik Dzhambazov Nikolay Zahariev |
| author_facet | Denitsa Kiradzhiyska Nikolina Milcheva Rositsa Mancheva Tsvetelina Batsalova Balik Dzhambazov Nikolay Zahariev |
| author_sort | Denitsa Kiradzhiyska |
| collection | DOAJ |
| description | The present study reports a specific method for preparation of silver-modified anodic alumina substrates intended for biomaterial applications. Al<sub>2</sub>O<sub>3</sub> coatings were obtained by anodization of technically pure aluminum alloy in sulfuric acid electrolyte. Silver deposition into the pores of the anodic structures was carried out employing in situ thermal reduction for different time periods. The obtained coatings were characterized using scanning electron microscopy (SEM), potentiodynamic scanning after 168 h in 3.5% NaCl solution and bioassays with human fibroblast and NIH/3T3 cell lines. The modified alumina substrates demonstrated better biocompatibility compared to the control anodic Al<sub>2</sub>O<sub>3</sub> pads indicated by increased percent cell survival following <i>in vitro</i> culture with human and mouse fibroblasts. The Ag-deposition time did not affect considerably the biocompatibility of the investigated anodic layers. SEM analyses indicated that mouse NIH/3T3 cells and human fibroblasts adhere to the silver-coated alumina substrates retaining normal morphology and ability to form cell monolayer. Therefore, the present studies demonstrate that silver coating of anodic alumina substrates improves their biocompatibility and their eventual biomedical application. |
| first_indexed | 2024-03-10T00:56:39Z |
| format | Article |
| id | doaj.art-5932cda4ea814c848dda7fe86cb255cc |
| institution | Directory Open Access Journal |
| issn | 2075-4701 |
| language | English |
| last_indexed | 2024-03-10T00:56:39Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metals |
| spelling | doaj.art-5932cda4ea814c848dda7fe86cb255cc2023-11-23T14:41:35ZengMDPI AGMetals2075-47012021-12-011215110.3390/met12010051Preparation and Preliminary Evaluation of Silver-Modified Anodic Alumina for Biomedical ApplicationsDenitsa Kiradzhiyska0Nikolina Milcheva1Rositsa Mancheva2Tsvetelina Batsalova3Balik Dzhambazov4Nikolay Zahariev5Department of Chemical Sciences, Faculty of Pharmacy, Medical University of Plovdiv, 15A Vassil Aprilov blvd., 4000 Plovdiv, BulgariaDepartment of Chemical Sciences, Faculty of Pharmacy, Medical University of Plovdiv, 15A Vassil Aprilov blvd., 4000 Plovdiv, BulgariaDepartment of Chemical Sciences, Faculty of Pharmacy, Medical University of Plovdiv, 15A Vassil Aprilov blvd., 4000 Plovdiv, BulgariaDepartment of Developmental Biology, Plovdiv University “Paisii Hilendarski”, 24 Tsar Assen Str., 4000 Plovdiv, BulgariaDepartment of Developmental Biology, Plovdiv University “Paisii Hilendarski”, 24 Tsar Assen Str., 4000 Plovdiv, BulgariaDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, Medical University of Plovdiv, 15A Vassil Aprilov blvd., 4000 Plovdiv, BulgariaThe present study reports a specific method for preparation of silver-modified anodic alumina substrates intended for biomaterial applications. Al<sub>2</sub>O<sub>3</sub> coatings were obtained by anodization of technically pure aluminum alloy in sulfuric acid electrolyte. Silver deposition into the pores of the anodic structures was carried out employing in situ thermal reduction for different time periods. The obtained coatings were characterized using scanning electron microscopy (SEM), potentiodynamic scanning after 168 h in 3.5% NaCl solution and bioassays with human fibroblast and NIH/3T3 cell lines. The modified alumina substrates demonstrated better biocompatibility compared to the control anodic Al<sub>2</sub>O<sub>3</sub> pads indicated by increased percent cell survival following <i>in vitro</i> culture with human and mouse fibroblasts. The Ag-deposition time did not affect considerably the biocompatibility of the investigated anodic layers. SEM analyses indicated that mouse NIH/3T3 cells and human fibroblasts adhere to the silver-coated alumina substrates retaining normal morphology and ability to form cell monolayer. Therefore, the present studies demonstrate that silver coating of anodic alumina substrates improves their biocompatibility and their eventual biomedical application.https://www.mdpi.com/2075-4701/12/1/51Ag/AAO substratesbiocompatibilityfibroblastsscanning electron microscopy |
| spellingShingle | Denitsa Kiradzhiyska Nikolina Milcheva Rositsa Mancheva Tsvetelina Batsalova Balik Dzhambazov Nikolay Zahariev Preparation and Preliminary Evaluation of Silver-Modified Anodic Alumina for Biomedical Applications Metals Ag/AAO substrates biocompatibility fibroblasts scanning electron microscopy |
| title | Preparation and Preliminary Evaluation of Silver-Modified Anodic Alumina for Biomedical Applications |
| title_full | Preparation and Preliminary Evaluation of Silver-Modified Anodic Alumina for Biomedical Applications |
| title_fullStr | Preparation and Preliminary Evaluation of Silver-Modified Anodic Alumina for Biomedical Applications |
| title_full_unstemmed | Preparation and Preliminary Evaluation of Silver-Modified Anodic Alumina for Biomedical Applications |
| title_short | Preparation and Preliminary Evaluation of Silver-Modified Anodic Alumina for Biomedical Applications |
| title_sort | preparation and preliminary evaluation of silver modified anodic alumina for biomedical applications |
| topic | Ag/AAO substrates biocompatibility fibroblasts scanning electron microscopy |
| url | https://www.mdpi.com/2075-4701/12/1/51 |
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