Isolation, identification and α-glucosidase inhibition evaluation of a phenolic compound from Elaeocarpus mastersii King leaves
Elaeocarpus mastersii King is an Indonesian folk medicine with high phenolic content. In this study, the E. mastersii King leaves were extracted using methanol solvent through maceration. The methanol extract was continuously fractionated by the liquid-liquid extraction using hexane, dichloromethane...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2024-01-01
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| Series: | E3S Web of Conferences |
| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2024/33/e3sconf_isac-iccme2023_07004.pdf |
| _version_ | 1797248942931443712 |
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| author | Okselni Tia Efdi Mai |
| author_facet | Okselni Tia Efdi Mai |
| author_sort | Okselni Tia |
| collection | DOAJ |
| description | Elaeocarpus mastersii King is an Indonesian folk medicine with high phenolic content. In this study, the E. mastersii King leaves were extracted using methanol solvent through maceration. The methanol extract was continuously fractionated by the liquid-liquid extraction using hexane, dichloromethane (DCM), and ethyl acetate solvents, respectively. The DCM fraction was purified by column chromatography using Silica gel 60 and Sephadex LH-20 as the stationary phase and monitored by thin-layer chromatography. This isolation process led to obtaining a pure compound with the appearance of white crystalline powder and a melting point of 198-199℃. This compound absorbed the UV light at the wavelength of 229 and 274 nm, as well as produced the bathochromic shift by the addition of NaOH reagent indicating the aromatic compound with the hydroxyl (OH) substituent. The IR spectrum confirmed the presence of aromatic and OH groups along with other functional groups, such as carbonyl (vmax 1693 cm-1) and alkane (C-H, vmax 2953 cm-1). The NMR data suggested the isolated compound as methyl gallate. Furthermore, the m/z value of the isolated compound was 185.04416 Da [M+H]+. It confirmed the monoisotopic mass of the methyl gallate (184.0362 Da). However, biological evaluation as an α-glucosidase inhibitor showed no positive activity. |
| first_indexed | 2024-04-24T20:22:37Z |
| format | Article |
| id | doaj.art-59333c30dcee4f899e21db3415b71d48 |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-04-24T20:22:37Z |
| publishDate | 2024-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-59333c30dcee4f899e21db3415b71d482024-03-22T07:56:07ZengEDP SciencesE3S Web of Conferences2267-12422024-01-015030700410.1051/e3sconf/202450307004e3sconf_isac-iccme2023_07004Isolation, identification and α-glucosidase inhibition evaluation of a phenolic compound from Elaeocarpus mastersii King leavesOkselni Tia0Efdi Mai1Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation AgencyDepartment of Chemistry, Universitas AndalasElaeocarpus mastersii King is an Indonesian folk medicine with high phenolic content. In this study, the E. mastersii King leaves were extracted using methanol solvent through maceration. The methanol extract was continuously fractionated by the liquid-liquid extraction using hexane, dichloromethane (DCM), and ethyl acetate solvents, respectively. The DCM fraction was purified by column chromatography using Silica gel 60 and Sephadex LH-20 as the stationary phase and monitored by thin-layer chromatography. This isolation process led to obtaining a pure compound with the appearance of white crystalline powder and a melting point of 198-199℃. This compound absorbed the UV light at the wavelength of 229 and 274 nm, as well as produced the bathochromic shift by the addition of NaOH reagent indicating the aromatic compound with the hydroxyl (OH) substituent. The IR spectrum confirmed the presence of aromatic and OH groups along with other functional groups, such as carbonyl (vmax 1693 cm-1) and alkane (C-H, vmax 2953 cm-1). The NMR data suggested the isolated compound as methyl gallate. Furthermore, the m/z value of the isolated compound was 185.04416 Da [M+H]+. It confirmed the monoisotopic mass of the methyl gallate (184.0362 Da). However, biological evaluation as an α-glucosidase inhibitor showed no positive activity.https://www.e3s-conferences.org/articles/e3sconf/pdf/2024/33/e3sconf_isac-iccme2023_07004.pdf |
| spellingShingle | Okselni Tia Efdi Mai Isolation, identification and α-glucosidase inhibition evaluation of a phenolic compound from Elaeocarpus mastersii King leaves E3S Web of Conferences |
| title | Isolation, identification and α-glucosidase inhibition evaluation of a phenolic compound from Elaeocarpus mastersii King leaves |
| title_full | Isolation, identification and α-glucosidase inhibition evaluation of a phenolic compound from Elaeocarpus mastersii King leaves |
| title_fullStr | Isolation, identification and α-glucosidase inhibition evaluation of a phenolic compound from Elaeocarpus mastersii King leaves |
| title_full_unstemmed | Isolation, identification and α-glucosidase inhibition evaluation of a phenolic compound from Elaeocarpus mastersii King leaves |
| title_short | Isolation, identification and α-glucosidase inhibition evaluation of a phenolic compound from Elaeocarpus mastersii King leaves |
| title_sort | isolation identification and α glucosidase inhibition evaluation of a phenolic compound from elaeocarpus mastersii king leaves |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2024/33/e3sconf_isac-iccme2023_07004.pdf |
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