Evidence for the circulating microRNA hsa-let-7d-3p as a potential new biomarker for sepsis in human subjects
Abstract Background Current biomarkers for the early detection of sepsis have low sensitivity and specificity. Serum microRNAs (miRNAs) have been proposed as novel noninvasive biomarkers for various diseases. The aim of the present study was to discover a novel diagnostic biomarker for sepsis in hum...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-07-01
|
| Series: | European Journal of Medical Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40001-022-00763-3 |
| _version_ | 1829098639348203520 |
|---|---|
| author | Zhaorui Zhang Hailun Luo Chunsun Li Zhixin Liang |
| author_facet | Zhaorui Zhang Hailun Luo Chunsun Li Zhixin Liang |
| author_sort | Zhaorui Zhang |
| collection | DOAJ |
| description | Abstract Background Current biomarkers for the early detection of sepsis have low sensitivity and specificity. Serum microRNAs (miRNAs) have been proposed as novel noninvasive biomarkers for various diseases. The aim of the present study was to discover a novel diagnostic biomarker for sepsis in human subjects. Methods miRNA expression profiling was performed using peripheral blood from three sepsis patients in the sepsis stage and improved condition stage using microarray screening. The differentially expressed miRNAs were primary validated by real-time quantitative polymerase chain reaction (RT-qPCR) in a further set of 20 sepsis patients in the sepsis stage and improved condition stage. Finally, we validated the differentially expressed miRNAs in 95 sepsis patients and 66 nonsepsis patients. The validated miRNAs and patients’ clinical indictors were analysed in a multivariate logistic regression model. The diagnostic value of the changed miRNA in sepsis was determined and compared with CRP and WBC by analysing the receiver operating characteristic (ROC) curves. Results According to the criteria, we detected 11 miRNAs regulated by the miRNA chip. RT-qPCR detection showed that the expression of hsa-let-7d-3p in sepsis patients was upregulated compared with that in nonsepsis patients. In a multiple logistic regression analysis, serum miRNA hsa-let-7d-3p was found to be an independent predictor of sepsis. Receiver operating characteristic curve (ROC) analysis showed that the area under the ROC curve of serum hsa-let-7d-3p was 0.696 [95% confidence interval (0.615, 0.778)]. Conclusion The miRNA hsa-let-7d-3p was identified as a novel biomarker for the early detection of sepsis. |
| first_indexed | 2024-12-10T21:12:18Z |
| format | Article |
| id | doaj.art-593346fc239341eb960177bbf3b76442 |
| institution | Directory Open Access Journal |
| issn | 2047-783X |
| language | English |
| last_indexed | 2024-12-10T21:12:18Z |
| publishDate | 2022-07-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj.art-593346fc239341eb960177bbf3b764422022-12-22T01:33:25ZengBMCEuropean Journal of Medical Research2047-783X2022-07-012711710.1186/s40001-022-00763-3Evidence for the circulating microRNA hsa-let-7d-3p as a potential new biomarker for sepsis in human subjectsZhaorui Zhang0Hailun Luo1Chunsun Li2Zhixin Liang3Department of Pulmonary and Critical Care Medicine, The Eighth Medical Center of Chinese, PLA General HospitalDepartment of Respiration, LiangXiang HospitalDepartment of Pulmonary and Critical Care Medicine, The Eighth Medical Center of Chinese, PLA General HospitalDepartment of Pulmonary and Critical Care Medicine, The Eighth Medical Center of Chinese, PLA General HospitalAbstract Background Current biomarkers for the early detection of sepsis have low sensitivity and specificity. Serum microRNAs (miRNAs) have been proposed as novel noninvasive biomarkers for various diseases. The aim of the present study was to discover a novel diagnostic biomarker for sepsis in human subjects. Methods miRNA expression profiling was performed using peripheral blood from three sepsis patients in the sepsis stage and improved condition stage using microarray screening. The differentially expressed miRNAs were primary validated by real-time quantitative polymerase chain reaction (RT-qPCR) in a further set of 20 sepsis patients in the sepsis stage and improved condition stage. Finally, we validated the differentially expressed miRNAs in 95 sepsis patients and 66 nonsepsis patients. The validated miRNAs and patients’ clinical indictors were analysed in a multivariate logistic regression model. The diagnostic value of the changed miRNA in sepsis was determined and compared with CRP and WBC by analysing the receiver operating characteristic (ROC) curves. Results According to the criteria, we detected 11 miRNAs regulated by the miRNA chip. RT-qPCR detection showed that the expression of hsa-let-7d-3p in sepsis patients was upregulated compared with that in nonsepsis patients. In a multiple logistic regression analysis, serum miRNA hsa-let-7d-3p was found to be an independent predictor of sepsis. Receiver operating characteristic curve (ROC) analysis showed that the area under the ROC curve of serum hsa-let-7d-3p was 0.696 [95% confidence interval (0.615, 0.778)]. Conclusion The miRNA hsa-let-7d-3p was identified as a novel biomarker for the early detection of sepsis.https://doi.org/10.1186/s40001-022-00763-3SepsisBiomarkersDiagnosisHsa-let-7d-3pmiRNAs |
| spellingShingle | Zhaorui Zhang Hailun Luo Chunsun Li Zhixin Liang Evidence for the circulating microRNA hsa-let-7d-3p as a potential new biomarker for sepsis in human subjects European Journal of Medical Research Sepsis Biomarkers Diagnosis Hsa-let-7d-3p miRNAs |
| title | Evidence for the circulating microRNA hsa-let-7d-3p as a potential new biomarker for sepsis in human subjects |
| title_full | Evidence for the circulating microRNA hsa-let-7d-3p as a potential new biomarker for sepsis in human subjects |
| title_fullStr | Evidence for the circulating microRNA hsa-let-7d-3p as a potential new biomarker for sepsis in human subjects |
| title_full_unstemmed | Evidence for the circulating microRNA hsa-let-7d-3p as a potential new biomarker for sepsis in human subjects |
| title_short | Evidence for the circulating microRNA hsa-let-7d-3p as a potential new biomarker for sepsis in human subjects |
| title_sort | evidence for the circulating microrna hsa let 7d 3p as a potential new biomarker for sepsis in human subjects |
| topic | Sepsis Biomarkers Diagnosis Hsa-let-7d-3p miRNAs |
| url | https://doi.org/10.1186/s40001-022-00763-3 |
| work_keys_str_mv | AT zhaoruizhang evidenceforthecirculatingmicrornahsalet7d3pasapotentialnewbiomarkerforsepsisinhumansubjects AT hailunluo evidenceforthecirculatingmicrornahsalet7d3pasapotentialnewbiomarkerforsepsisinhumansubjects AT chunsunli evidenceforthecirculatingmicrornahsalet7d3pasapotentialnewbiomarkerforsepsisinhumansubjects AT zhixinliang evidenceforthecirculatingmicrornahsalet7d3pasapotentialnewbiomarkerforsepsisinhumansubjects |