Presence and antibiotic resistance of listeria monocytogenes in raw milk and dairy products
In this study, the presence of Listeria monocytogenes in raw milk and milk products produced from raw milk by traditional method, such as cheese and butter using cultural and molecular techniques and the antibiotic resistances were investigated. Isolation was performed using the FDA technique. Micro...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Kafkas University, Faculty of Veterinary Medicine
2018-03-01
|
| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=2349 |
| _version_ | 1797800638761926656 |
|---|---|
| author | Aksem AKSOY Çiğdem SEZER Leyla VATANSEVER Güven GÜLBAZ |
| author_facet | Aksem AKSOY Çiğdem SEZER Leyla VATANSEVER Güven GÜLBAZ |
| author_sort | Aksem AKSOY |
| collection | DOAJ |
| description | In this study, the presence of Listeria monocytogenes in raw milk and milk products produced from raw milk by traditional method, such as
cheese and butter using cultural and molecular techniques and the antibiotic resistances were investigated. Isolation was performed using
the FDA technique. Microbact 12L test kit was used for biochemical identification. Genotyping of isolates was done by traditional PCR method.
Antibiotic resistance of isolates was determined by standard disk diffusion method. Listeria spp. was detected in 16 (5.3%) of the total 300 food
samples according to the biochemical identification results. The highest prevalence of Listeria spp. was in raw milk samples (10%), followed by
cheese samples (4%) and butter samples (2%). All 36 Listeria spp. isolates which were obtained from biochemical identification were tested for
L. monocytogenes by PCR, and 15 (41.6%) of them were confirmed as L. monocytogenes. According to the antibiotic resistance test results of L.
monocytogenes strains, four (26.7%) isolates were resistant to at least one antibiotic while only one (6.7%) had multiple antibiotic resistance.
The highest resistance was found against trimethoprim-sulfamethoxazole. It has been determined that L. monocytogenes isolates had the same
degree resistance against penicillin G, meropenem, amikacin and vancomycin. In conclusion, the presence of L. monocytogenes which has
been detected in dairy products made from raw milk and in raw milk itself may constitute a potential risk for public health. Furthermore, it is
threatening for public health that the isolates obtained are resistant to certain antibiotics. |
| first_indexed | 2024-03-13T04:37:16Z |
| format | Article |
| id | doaj.art-59377208146d4746bab505efdcb7d242 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:37:16Z |
| publishDate | 2018-03-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-59377208146d4746bab505efdcb7d2422023-06-19T06:43:42ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512018-03-0124341542110.9775/kvfd.2017.190812349Presence and antibiotic resistance of listeria monocytogenes in raw milk and dairy productsAksem AKSOY0Çiğdem SEZER1Leyla VATANSEVER2Güven GÜLBAZ3Kafkas University, Faculty of Engineering Architecture, Department of Food Engineering, TR-36100 Kars - TURKEYKafkas University, Faculty of Veterinary Medicine, Department of Food Safety and Public Health, TR-36100 Kars - TURKEYKafkas University, Faculty of Veterinary Medicine, Department of Food Safety and Public Health, TR-36100 Kars - TURKEYKafkas University, Faculty of Engineering Architecture, Department of Food Engineering, TR-36100 Kars - TURKEYIn this study, the presence of Listeria monocytogenes in raw milk and milk products produced from raw milk by traditional method, such as cheese and butter using cultural and molecular techniques and the antibiotic resistances were investigated. Isolation was performed using the FDA technique. Microbact 12L test kit was used for biochemical identification. Genotyping of isolates was done by traditional PCR method. Antibiotic resistance of isolates was determined by standard disk diffusion method. Listeria spp. was detected in 16 (5.3%) of the total 300 food samples according to the biochemical identification results. The highest prevalence of Listeria spp. was in raw milk samples (10%), followed by cheese samples (4%) and butter samples (2%). All 36 Listeria spp. isolates which were obtained from biochemical identification were tested for L. monocytogenes by PCR, and 15 (41.6%) of them were confirmed as L. monocytogenes. According to the antibiotic resistance test results of L. monocytogenes strains, four (26.7%) isolates were resistant to at least one antibiotic while only one (6.7%) had multiple antibiotic resistance. The highest resistance was found against trimethoprim-sulfamethoxazole. It has been determined that L. monocytogenes isolates had the same degree resistance against penicillin G, meropenem, amikacin and vancomycin. In conclusion, the presence of L. monocytogenes which has been detected in dairy products made from raw milk and in raw milk itself may constitute a potential risk for public health. Furthermore, it is threatening for public health that the isolates obtained are resistant to certain antibiotics.https://vetdergikafkas.org/pdf.php?id=2349milkdairy productl. monocytogeneslisteria spp.antibiotic resistance |
| spellingShingle | Aksem AKSOY Çiğdem SEZER Leyla VATANSEVER Güven GÜLBAZ Presence and antibiotic resistance of listeria monocytogenes in raw milk and dairy products Kafkas Universitesi Veteriner Fakültesi Dergisi milk dairy product l. monocytogenes listeria spp. antibiotic resistance |
| title | Presence and antibiotic resistance of listeria monocytogenes in raw milk and dairy products |
| title_full | Presence and antibiotic resistance of listeria monocytogenes in raw milk and dairy products |
| title_fullStr | Presence and antibiotic resistance of listeria monocytogenes in raw milk and dairy products |
| title_full_unstemmed | Presence and antibiotic resistance of listeria monocytogenes in raw milk and dairy products |
| title_short | Presence and antibiotic resistance of listeria monocytogenes in raw milk and dairy products |
| title_sort | presence and antibiotic resistance of listeria monocytogenes in raw milk and dairy products |
| topic | milk dairy product l. monocytogenes listeria spp. antibiotic resistance |
| url | https://vetdergikafkas.org/pdf.php?id=2349 |
| work_keys_str_mv | AT aksemaksoy presenceandantibioticresistanceoflisteriamonocytogenesinrawmilkanddairyproducts AT cigdemsezer presenceandantibioticresistanceoflisteriamonocytogenesinrawmilkanddairyproducts AT leylavatansever presenceandantibioticresistanceoflisteriamonocytogenesinrawmilkanddairyproducts AT guvengulbaz presenceandantibioticresistanceoflisteriamonocytogenesinrawmilkanddairyproducts |