Anti-allergic activities of Umbelliferone against histamine- and Picryl chloride-induced ear edema by targeting Nrf2/iNOS signaling in mice
Abstract Background The current study was aimed to investigate the anti-allergic activities of the Umbelliferone (UMB) against the acute Histamine and chronic Picryl chloride (PiCl)-induced allergy in mice. UMB is a coumarin derivative (isolated from Angelica decursiva) found in various parts of the...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2021-08-01
|
| Series: | BMC Complementary Medicine and Therapies |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12906-021-03384-1 |
| _version_ | 1818910719691718656 |
|---|---|
| author | Younas Ashrafullah Khan Omer Shehzad Eun Kyoung Seo Alev Onder Salman Khan |
| author_facet | Younas Ashrafullah Khan Omer Shehzad Eun Kyoung Seo Alev Onder Salman Khan |
| author_sort | Younas |
| collection | DOAJ |
| description | Abstract Background The current study was aimed to investigate the anti-allergic activities of the Umbelliferone (UMB) against the acute Histamine and chronic Picryl chloride (PiCl)-induced allergy in mice. UMB is a coumarin derivative (isolated from Angelica decursiva) found in various parts of the plants such as flowers, roots and, stems isolated from the plants of Umbelliferae family. Methods The UMB (1, 10, 50 mg/kg) was administered intraperitoneally (i.p) half an h before or 2 h after the induction of allergic ear edema. The acute ear edema was induced by histamine (intradermally, i.d), while the chronic ear edema was induced by painting the PiCl (sensitized with the toluene) on the ear. The antioxidants and oxidative stress markers were assessed. The histological changes were assessed using Hematoxylin and eosin (H and E) and giemsa staining. The immunohistochemistry studies were performed to assess the expression of the nuclear factor erythroid 2-related factor 2 (Nrf2) and inducible nitric oxide synthase (iNOS). The data was analyzed using one-way ANOVA tests followed by Tukey’s test with p < 0.05 was chosen as criteria for statistical significance. Results UMB treatment markedly reduced the allergic ear edema and ear weight compared to the negative control. Furthermore, the UMB attenuated the oxidative stress markers, while induced the antioxidants enzymes. Similarly, the UMB treatment significantly attenuated the serum immunoglobulin E (IgE) level. The UMB treatment markedly improved the histological parameters using H and E staining and Giemsa staining. The UMB administration induced the Nrf2 expression, while attenuated the iNOS expression. Furthermore, the computational analysis was performed to assess the interaction of the UMB with the various protein targets and to determine the mechanism of interaction with the target proteins. Conclusion In conclusion, the UMB treatment significantly alleviated the allergic symptoms, attenuating the oxidative stress, improved the histological features using in vivo and computational approaches. |
| first_indexed | 2024-12-19T22:47:17Z |
| format | Article |
| id | doaj.art-5939a43533e34e2baa17aab0a7278f98 |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-12-19T22:47:17Z |
| publishDate | 2021-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-5939a43533e34e2baa17aab0a7278f982022-12-21T20:02:55ZengBMCBMC Complementary Medicine and Therapies2662-76712021-08-0121111710.1186/s12906-021-03384-1Anti-allergic activities of Umbelliferone against histamine- and Picryl chloride-induced ear edema by targeting Nrf2/iNOS signaling in miceYounas0Ashrafullah Khan1Omer Shehzad2Eun Kyoung Seo3Alev Onder4Salman Khan5Pharmacological Sciences Research Lab, Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University IslamabadPharmacological Sciences Research Lab, Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University IslamabadDepartment of Pharmacy, Abdul Wali Khan UniversityCollege of Pharmacy, Ewha Womans UniversityDepartment of Pharmacognosy, Faculty of Pharmacy, Ankara UniversityPharmacological Sciences Research Lab, Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University IslamabadAbstract Background The current study was aimed to investigate the anti-allergic activities of the Umbelliferone (UMB) against the acute Histamine and chronic Picryl chloride (PiCl)-induced allergy in mice. UMB is a coumarin derivative (isolated from Angelica decursiva) found in various parts of the plants such as flowers, roots and, stems isolated from the plants of Umbelliferae family. Methods The UMB (1, 10, 50 mg/kg) was administered intraperitoneally (i.p) half an h before or 2 h after the induction of allergic ear edema. The acute ear edema was induced by histamine (intradermally, i.d), while the chronic ear edema was induced by painting the PiCl (sensitized with the toluene) on the ear. The antioxidants and oxidative stress markers were assessed. The histological changes were assessed using Hematoxylin and eosin (H and E) and giemsa staining. The immunohistochemistry studies were performed to assess the expression of the nuclear factor erythroid 2-related factor 2 (Nrf2) and inducible nitric oxide synthase (iNOS). The data was analyzed using one-way ANOVA tests followed by Tukey’s test with p < 0.05 was chosen as criteria for statistical significance. Results UMB treatment markedly reduced the allergic ear edema and ear weight compared to the negative control. Furthermore, the UMB attenuated the oxidative stress markers, while induced the antioxidants enzymes. Similarly, the UMB treatment significantly attenuated the serum immunoglobulin E (IgE) level. The UMB treatment markedly improved the histological parameters using H and E staining and Giemsa staining. The UMB administration induced the Nrf2 expression, while attenuated the iNOS expression. Furthermore, the computational analysis was performed to assess the interaction of the UMB with the various protein targets and to determine the mechanism of interaction with the target proteins. Conclusion In conclusion, the UMB treatment significantly alleviated the allergic symptoms, attenuating the oxidative stress, improved the histological features using in vivo and computational approaches.https://doi.org/10.1186/s12906-021-03384-1UmbelliferonePicryl chlorideOxidative stressAntioxidantInflammation |
| spellingShingle | Younas Ashrafullah Khan Omer Shehzad Eun Kyoung Seo Alev Onder Salman Khan Anti-allergic activities of Umbelliferone against histamine- and Picryl chloride-induced ear edema by targeting Nrf2/iNOS signaling in mice BMC Complementary Medicine and Therapies Umbelliferone Picryl chloride Oxidative stress Antioxidant Inflammation |
| title | Anti-allergic activities of Umbelliferone against histamine- and Picryl chloride-induced ear edema by targeting Nrf2/iNOS signaling in mice |
| title_full | Anti-allergic activities of Umbelliferone against histamine- and Picryl chloride-induced ear edema by targeting Nrf2/iNOS signaling in mice |
| title_fullStr | Anti-allergic activities of Umbelliferone against histamine- and Picryl chloride-induced ear edema by targeting Nrf2/iNOS signaling in mice |
| title_full_unstemmed | Anti-allergic activities of Umbelliferone against histamine- and Picryl chloride-induced ear edema by targeting Nrf2/iNOS signaling in mice |
| title_short | Anti-allergic activities of Umbelliferone against histamine- and Picryl chloride-induced ear edema by targeting Nrf2/iNOS signaling in mice |
| title_sort | anti allergic activities of umbelliferone against histamine and picryl chloride induced ear edema by targeting nrf2 inos signaling in mice |
| topic | Umbelliferone Picryl chloride Oxidative stress Antioxidant Inflammation |
| url | https://doi.org/10.1186/s12906-021-03384-1 |
| work_keys_str_mv | AT younas antiallergicactivitiesofumbelliferoneagainsthistamineandpicrylchlorideinducedearedemabytargetingnrf2inossignalinginmice AT ashrafullahkhan antiallergicactivitiesofumbelliferoneagainsthistamineandpicrylchlorideinducedearedemabytargetingnrf2inossignalinginmice AT omershehzad antiallergicactivitiesofumbelliferoneagainsthistamineandpicrylchlorideinducedearedemabytargetingnrf2inossignalinginmice AT eunkyoungseo antiallergicactivitiesofumbelliferoneagainsthistamineandpicrylchlorideinducedearedemabytargetingnrf2inossignalinginmice AT alevonder antiallergicactivitiesofumbelliferoneagainsthistamineandpicrylchlorideinducedearedemabytargetingnrf2inossignalinginmice AT salmankhan antiallergicactivitiesofumbelliferoneagainsthistamineandpicrylchlorideinducedearedemabytargetingnrf2inossignalinginmice |