Qiangguyin inhibited fat accumulation in OVX mice through the p38 MAPK signaling pathway to achieve anti-osteoporosis effects
Postmenopausal osteoporosis (PMOP) is a common bone disease characterized by decreased bone density and increased bone fragility due to decreased estrogen levels. Qiangguyin (QGY) is transformed from the famous traditional Chinese medicine BuShen Invigorating Blood Decoction. In this study, we used...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | Biomedicine & Pharmacotherapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332222015116 |
| _version_ | 1811177277711450112 |
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| author | Jingyuan Wen Zhengsheng Bao Lunxin Li Yingquan Liu Bing Wei Xiaoang Ye Huihui Xu Longkang Cui Xuefei Li Gaobo Shen Yuan Fang Hanbing Zeng Zhe Shen Enping Guo Hongting Jin Lianguo Wu |
| author_facet | Jingyuan Wen Zhengsheng Bao Lunxin Li Yingquan Liu Bing Wei Xiaoang Ye Huihui Xu Longkang Cui Xuefei Li Gaobo Shen Yuan Fang Hanbing Zeng Zhe Shen Enping Guo Hongting Jin Lianguo Wu |
| author_sort | Jingyuan Wen |
| collection | DOAJ |
| description | Postmenopausal osteoporosis (PMOP) is a common bone disease characterized by decreased bone density and increased bone fragility due to decreased estrogen levels. Qiangguyin (QGY) is transformed from the famous traditional Chinese medicine BuShen Invigorating Blood Decoction. In this study, we used QGY to treat PMOP. We observed that QGY significantly reduced fat accumulation in the chondro-osseous junction. However, its specific mechanism of action remains unclear. To determine the specific molecular mechanism of QGY, we explored the pharmacological mechanism by which QGY reduces fat accumulation in the chondro-osseous junction through network pharmacological analysis. The active components and targets related to PMOP and QGY were screened from different databases, forming a composition-target-disease network. Next, a comprehensive analysis platform including protein-protein interaction (PPI) network, Gene Ontology (GO) enrichment analysis, and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were established. The results revealed that QGY inhibits adipogenic differentiation by activating the mitogen-activated protein kinase (MAPK) signaling pathway, thus reducing the accumulation of fat in the chondro-osseous junction. For further verification. In vitro and in vivo experiments were carried out. Our data showed that QGY significantly reversed the high expression of fatty acid binding protein 4 (FABP4) and peroxisome proliferator-activated receptor γ (PPARγ). Further, QGY prevents fat accumulation by inhibiting the expression of p38. In summary, the results of this study suggested that QGY-induced phenotypic changes are related to the activation of the p38 MAPK signaling pathway. |
| first_indexed | 2024-04-10T22:58:59Z |
| format | Article |
| id | doaj.art-593b8e809b0949448b0a4e9fb024cbef |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-04-10T22:58:59Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-593b8e809b0949448b0a4e9fb024cbef2023-01-14T04:25:52ZengElsevierBiomedicine & Pharmacotherapy0753-33222023-02-01158114122Qiangguyin inhibited fat accumulation in OVX mice through the p38 MAPK signaling pathway to achieve anti-osteoporosis effectsJingyuan Wen0Zhengsheng Bao1Lunxin Li2Yingquan Liu3Bing Wei4Xiaoang Ye5Huihui Xu6Longkang Cui7Xuefei Li8Gaobo Shen9Yuan Fang10Hanbing Zeng11Zhe Shen12Enping Guo13Hongting Jin14Lianguo Wu15The Second Clinical College, Zhejiang Chinese Medical University, Hangzhou, China; The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe Second Clinical College, Zhejiang Chinese Medical University, Hangzhou, China; The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe Second Clinical College, Zhejiang Chinese Medical University, Hangzhou, China; The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe Second Clinical College, Zhejiang Chinese Medical University, Hangzhou, China; The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe Second Clinical College, Zhejiang Chinese Medical University, Hangzhou, China; The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe First Clinical College, Zhejiang Chinese Medical University, Hangzhou, ChinaThe First Clinical College, Zhejiang Chinese Medical University, Hangzhou, ChinaThe Second Clinical College, Zhejiang Chinese Medical University, Hangzhou, China; The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe Second Clinical College, Zhejiang Chinese Medical University, Hangzhou, China; The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe Second Clinical College, Zhejiang Chinese Medical University, Hangzhou, China; The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe Second Clinical College, Zhejiang Chinese Medical University, Hangzhou, China; The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe Second Clinical College, Zhejiang Chinese Medical University, Hangzhou, China; The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe First Clinical College, Zhejiang Chinese Medical University, Hangzhou, ChinaThe Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, ChinaThe First Clinical College, Zhejiang Chinese Medical University, Hangzhou, China; Corresponding authors.The Second Clinical College, Zhejiang Chinese Medical University, Hangzhou, China; Corresponding authors.Postmenopausal osteoporosis (PMOP) is a common bone disease characterized by decreased bone density and increased bone fragility due to decreased estrogen levels. Qiangguyin (QGY) is transformed from the famous traditional Chinese medicine BuShen Invigorating Blood Decoction. In this study, we used QGY to treat PMOP. We observed that QGY significantly reduced fat accumulation in the chondro-osseous junction. However, its specific mechanism of action remains unclear. To determine the specific molecular mechanism of QGY, we explored the pharmacological mechanism by which QGY reduces fat accumulation in the chondro-osseous junction through network pharmacological analysis. The active components and targets related to PMOP and QGY were screened from different databases, forming a composition-target-disease network. Next, a comprehensive analysis platform including protein-protein interaction (PPI) network, Gene Ontology (GO) enrichment analysis, and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were established. The results revealed that QGY inhibits adipogenic differentiation by activating the mitogen-activated protein kinase (MAPK) signaling pathway, thus reducing the accumulation of fat in the chondro-osseous junction. For further verification. In vitro and in vivo experiments were carried out. Our data showed that QGY significantly reversed the high expression of fatty acid binding protein 4 (FABP4) and peroxisome proliferator-activated receptor γ (PPARγ). Further, QGY prevents fat accumulation by inhibiting the expression of p38. In summary, the results of this study suggested that QGY-induced phenotypic changes are related to the activation of the p38 MAPK signaling pathway.http://www.sciencedirect.com/science/article/pii/S0753332222015116QiangguyinP38 MAPKPostmenopausal osteoporosisLipid differentiationChondro-osseous junction |
| spellingShingle | Jingyuan Wen Zhengsheng Bao Lunxin Li Yingquan Liu Bing Wei Xiaoang Ye Huihui Xu Longkang Cui Xuefei Li Gaobo Shen Yuan Fang Hanbing Zeng Zhe Shen Enping Guo Hongting Jin Lianguo Wu Qiangguyin inhibited fat accumulation in OVX mice through the p38 MAPK signaling pathway to achieve anti-osteoporosis effects Biomedicine & Pharmacotherapy Qiangguyin P38 MAPK Postmenopausal osteoporosis Lipid differentiation Chondro-osseous junction |
| title | Qiangguyin inhibited fat accumulation in OVX mice through the p38 MAPK signaling pathway to achieve anti-osteoporosis effects |
| title_full | Qiangguyin inhibited fat accumulation in OVX mice through the p38 MAPK signaling pathway to achieve anti-osteoporosis effects |
| title_fullStr | Qiangguyin inhibited fat accumulation in OVX mice through the p38 MAPK signaling pathway to achieve anti-osteoporosis effects |
| title_full_unstemmed | Qiangguyin inhibited fat accumulation in OVX mice through the p38 MAPK signaling pathway to achieve anti-osteoporosis effects |
| title_short | Qiangguyin inhibited fat accumulation in OVX mice through the p38 MAPK signaling pathway to achieve anti-osteoporosis effects |
| title_sort | qiangguyin inhibited fat accumulation in ovx mice through the p38 mapk signaling pathway to achieve anti osteoporosis effects |
| topic | Qiangguyin P38 MAPK Postmenopausal osteoporosis Lipid differentiation Chondro-osseous junction |
| url | http://www.sciencedirect.com/science/article/pii/S0753332222015116 |
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