Comparative Evaluation of Biofilm Detection Methods among Uropathogenic Gram-Positive and Gram Negative Bacteria Isolated from Catheterized Urine Sample and its antibiogram
Background: Biofilm formation is apotential virulence characteristic exhibitedby bacteria resulting in theseverity of many infections. It will give rise to multidrug resistant strains disturbing the effective management of many chronic infections. Objectives: The present study aims to focus on the...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
B.J.Medical College Development Society, Ahmedabad
2022-12-01
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| Series: | BJ Kines: National Journal of Basic & Applied Sciences |
| Subjects: | |
| Online Access: | https://bjkines.com/vol14(2)December2022/article%209.pdf |
| Summary: | Background: Biofilm formation is apotential virulence characteristic exhibitedby bacteria resulting in
theseverity of many infections. It will give rise to multidrug resistant strains disturbing the effective
management of many chronic infections. Objectives: The present study aims to focus on the biofilm
detection of uropathogenic bacteria by three distinct techniques. The sensitivity and specificity for all the
three methods is evaluated. Thepresent study also demonstrates the antibiogram of biofilm producing
bacteria. Materials and Method: The study includes 89bacterial isolates from catheterized patients with
urinary tract infections admitted in ICUs, dialysis units and various wards. Formation of biofilm was
detected by: tube method (TM), the tissue culture plate (TCP) method and Congo Red Agar (CRA)
method. According to CLSI guidelines the antimicrobial susceptibility test was performed among the
biofilm forming bacteria. Results: Out of 89 bacterial isolates, 33 were gram positive cocci and 56 were
gram negative bacilli. Among the gram positive cocci, 28 (84.8%) andgram negative bacilli, 50(89.2%)
formed biofilm. Sensitivity of TCM, TM, and CRM methods were 98.3%, 71.4% and 18.6% respectively.
For biofilm forming gram positive bacteria, the maximum antibiotic resistance was achieved towards
Penicillin (89%) followed by Amoxyclav (78%). In the case of gram negative bacteria gentamicin showed
maximum resistance in 88% followed by Ciprofloxacin in 80% of the isolates. Conclusion: Biofilm and
multi-drug resistance plays a vital role in thepathogenesis ofcatheterized urinary tract infection (CAUTI).
Evaluation of Biofilm among uropathogenic bacteria helps to manage clinically resulting in better
prognosis. Tissue culture plate techniqueisvery effectivein detecting biofilm that can be suggested in
diagnostic laboratories. |
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| ISSN: | 2231-6140 2395-7859 |