Identification of polymorphic loci in the deiodinase 2 gene and their associations with head dimensions in geese
Objective This study was conducted to clone and compare the molecular characteristics of the deiodinase 2 (DIO2) gene between Sichuan White geese and Landes geese, and to analyze the association between polymorphisms of the DIO2 gene and head dimensions in Tianfu meat geese. Methods The coding seque...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2022-05-01
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| Series: | Animal Bioscience |
| Subjects: | |
| Online Access: | http://www.animbiosci.org/upload/pdf/ab-21-0382.pdf |
| _version_ | 1811252635069579264 |
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| author | Yan Deng Qian Hu Bincheng Tang Qingyuan Ouyang Shenqiang Hu Bo Hu Jiwei Hu Hua He Guohong Chen Jiwen Wang |
| author_facet | Yan Deng Qian Hu Bincheng Tang Qingyuan Ouyang Shenqiang Hu Bo Hu Jiwei Hu Hua He Guohong Chen Jiwen Wang |
| author_sort | Yan Deng |
| collection | DOAJ |
| description | Objective This study was conducted to clone and compare the molecular characteristics of the deiodinase 2 (DIO2) gene between Sichuan White geese and Landes geese, and to analyze the association between polymorphisms of the DIO2 gene and head dimensions in Tianfu meat geese. Methods The coding sequence of the DIO2 gene was cloned by polymerase chain reaction and vector ligation and aligned by DNAMAN software. A total of 350 Tianfu meat geese were used to genotype the polymorphisms of the DIO2 gene and measure the head dimensions. Association analysis between the polymorphisms of the DIO2 gene and head dimensions was carried out. Results An 840-bp coding sequence of the DIO2 gene was obtained and comparison analysis identified four polymorphic loci between Sichuan White geese and Landes geese. Further analysis showed that the dominant alleles for the four polymorphic loci were G, G, A, and T and the frequency of the heterozygous genotype was higher than that of the homozygous genotype in Tianfu meat geese. Compared to that in the population of non-knob geese of Tianfu meat geese, the head dimensions in the population of knob geese were significantly higher except for nostril height. However, in the non-knob geese, beak width 1, beak width 2, nostril length, cranial width 1, and maxillary length had significant differences among different genotypes or haplotypes/diplotypes. Conclusion These results suggested that polymorphisms of the DIO2 gene could be considered molecular markers to select larger heads of geese in the population of non-knob geese. |
| first_indexed | 2024-04-12T16:38:35Z |
| format | Article |
| id | doaj.art-593e44d1c2b84f3883ec0f84018a8cb4 |
| institution | Directory Open Access Journal |
| issn | 2765-0189 2765-0235 |
| language | English |
| last_indexed | 2024-04-12T16:38:35Z |
| publishDate | 2022-05-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj.art-593e44d1c2b84f3883ec0f84018a8cb42022-12-22T03:24:54ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352022-05-0135563964710.5713/ab.21.038224792Identification of polymorphic loci in the deiodinase 2 gene and their associations with head dimensions in geeseYan Deng0Qian Hu1Bincheng Tang2Qingyuan Ouyang3Shenqiang Hu4Bo Hu5Jiwei Hu6Hua He7Guohong Chen8Jiwen Wang9 Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, China Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, China Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, China Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, China Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, China Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, China Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, China Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, China Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225000, Jiangsu, China Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, ChinaObjective This study was conducted to clone and compare the molecular characteristics of the deiodinase 2 (DIO2) gene between Sichuan White geese and Landes geese, and to analyze the association between polymorphisms of the DIO2 gene and head dimensions in Tianfu meat geese. Methods The coding sequence of the DIO2 gene was cloned by polymerase chain reaction and vector ligation and aligned by DNAMAN software. A total of 350 Tianfu meat geese were used to genotype the polymorphisms of the DIO2 gene and measure the head dimensions. Association analysis between the polymorphisms of the DIO2 gene and head dimensions was carried out. Results An 840-bp coding sequence of the DIO2 gene was obtained and comparison analysis identified four polymorphic loci between Sichuan White geese and Landes geese. Further analysis showed that the dominant alleles for the four polymorphic loci were G, G, A, and T and the frequency of the heterozygous genotype was higher than that of the homozygous genotype in Tianfu meat geese. Compared to that in the population of non-knob geese of Tianfu meat geese, the head dimensions in the population of knob geese were significantly higher except for nostril height. However, in the non-knob geese, beak width 1, beak width 2, nostril length, cranial width 1, and maxillary length had significant differences among different genotypes or haplotypes/diplotypes. Conclusion These results suggested that polymorphisms of the DIO2 gene could be considered molecular markers to select larger heads of geese in the population of non-knob geese.http://www.animbiosci.org/upload/pdf/ab-21-0382.pdfdeiodinase 2geesehead dimensionsknobpolymorphic loci |
| spellingShingle | Yan Deng Qian Hu Bincheng Tang Qingyuan Ouyang Shenqiang Hu Bo Hu Jiwei Hu Hua He Guohong Chen Jiwen Wang Identification of polymorphic loci in the deiodinase 2 gene and their associations with head dimensions in geese Animal Bioscience deiodinase 2 geese head dimensions knob polymorphic loci |
| title | Identification of polymorphic loci in the deiodinase 2 gene and their associations with head dimensions in geese |
| title_full | Identification of polymorphic loci in the deiodinase 2 gene and their associations with head dimensions in geese |
| title_fullStr | Identification of polymorphic loci in the deiodinase 2 gene and their associations with head dimensions in geese |
| title_full_unstemmed | Identification of polymorphic loci in the deiodinase 2 gene and their associations with head dimensions in geese |
| title_short | Identification of polymorphic loci in the deiodinase 2 gene and their associations with head dimensions in geese |
| title_sort | identification of polymorphic loci in the deiodinase 2 gene and their associations with head dimensions in geese |
| topic | deiodinase 2 geese head dimensions knob polymorphic loci |
| url | http://www.animbiosci.org/upload/pdf/ab-21-0382.pdf |
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