Effect of Plasma Treatment of Titanium Surface on Biocompatibility
It was recently reported that implant osseointegration is affected by surface wettability. The relationship between hydrophilicity and cell adhesion was corroborated by numerous in vivo studies. Concentrated alkali improves the biocompatibility of pure titanium. Research was conducted on the mechani...
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MDPI AG
2019-05-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/9/11/2257 |
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| author | Daiga Ujino Hiroshi Nishizaki Shizuo Higuchi Satoshi Komasa Joji Okazaki |
| author_facet | Daiga Ujino Hiroshi Nishizaki Shizuo Higuchi Satoshi Komasa Joji Okazaki |
| author_sort | Daiga Ujino |
| collection | DOAJ |
| description | It was recently reported that implant osseointegration is affected by surface wettability. The relationship between hydrophilicity and cell adhesion was corroborated by numerous in vivo studies. Concentrated alkali improves the biocompatibility of pure titanium. Research was conducted on the mechanism by which this treatment increases hydrophilicity. In the present study, we used atmospheric pressure plasma processing to enhance the hydrophilicity of the material surface. The aim was to assess its influences on the initial adhesion of the material to rat bone marrow and subsequent differentiation into hard tissue. Superhydrophilicity was induced on a pure titanium surface with a piezobrush, a simple, compact alternative to the conventional atmospheric pressure plasma device. No structural change was confirmed by Scanning electron microscope (SEM) or scanning probe microscopy (SPM) observation. X-ray photoelectron spectroscopy (XPS) analysis presented with hydroxide formation and a reduction in the C peak. A decrease in contact angle was also observed. The treated samples had higher values for in vitro bovine serum albumin (BSA) adsorption, rat bone marrow (RBM) cell initial adhesion, alkaline phosphatase activity (ALP) activity tests, and factors related to bone differentiation than the untreated control. The present study indicated that the induction of superhydrophilicity in titanium via atmospheric pressure plasma treatment with a piezobrush affects RBM cell adhesion and bone differentiation without altering surface properties. |
| first_indexed | 2024-12-19T08:31:52Z |
| format | Article |
| id | doaj.art-593f28dea095405f9245beb6cc41e5a8 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-12-19T08:31:52Z |
| publishDate | 2019-05-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-593f28dea095405f9245beb6cc41e5a82022-12-21T20:29:10ZengMDPI AGApplied Sciences2076-34172019-05-01911225710.3390/app9112257app9112257Effect of Plasma Treatment of Titanium Surface on BiocompatibilityDaiga Ujino0Hiroshi Nishizaki1Shizuo Higuchi2Satoshi Komasa3Joji Okazaki4Master’s Course of Oral Sciences, Graduate School of Health Sciences, Osaka Dental University, Osaka 573-1144, JapanDepartment of Oral Health Engeering, Faculty of Health Sciences, Osaka Dental University, 1-4-4, Makino-honmachi, Hirakata-shi, Osaka 573-1144, JapanDepartment of Oral Health Engeering, Faculty of Health Sciences, Osaka Dental University, 1-4-4, Makino-honmachi, Hirakata-shi, Osaka 573-1144, JapanDepartment of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1, Kuzuhahanazono-cho, Hirakata-shi, Osaka 573-1121, JapanDepartment of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1, Kuzuhahanazono-cho, Hirakata-shi, Osaka 573-1121, JapanIt was recently reported that implant osseointegration is affected by surface wettability. The relationship between hydrophilicity and cell adhesion was corroborated by numerous in vivo studies. Concentrated alkali improves the biocompatibility of pure titanium. Research was conducted on the mechanism by which this treatment increases hydrophilicity. In the present study, we used atmospheric pressure plasma processing to enhance the hydrophilicity of the material surface. The aim was to assess its influences on the initial adhesion of the material to rat bone marrow and subsequent differentiation into hard tissue. Superhydrophilicity was induced on a pure titanium surface with a piezobrush, a simple, compact alternative to the conventional atmospheric pressure plasma device. No structural change was confirmed by Scanning electron microscope (SEM) or scanning probe microscopy (SPM) observation. X-ray photoelectron spectroscopy (XPS) analysis presented with hydroxide formation and a reduction in the C peak. A decrease in contact angle was also observed. The treated samples had higher values for in vitro bovine serum albumin (BSA) adsorption, rat bone marrow (RBM) cell initial adhesion, alkaline phosphatase activity (ALP) activity tests, and factors related to bone differentiation than the untreated control. The present study indicated that the induction of superhydrophilicity in titanium via atmospheric pressure plasma treatment with a piezobrush affects RBM cell adhesion and bone differentiation without altering surface properties.https://www.mdpi.com/2076-3417/9/11/2257osseointegrationpiezobrushrat bone marrow cellsuperhydrophilicity |
| spellingShingle | Daiga Ujino Hiroshi Nishizaki Shizuo Higuchi Satoshi Komasa Joji Okazaki Effect of Plasma Treatment of Titanium Surface on Biocompatibility Applied Sciences osseointegration piezobrush rat bone marrow cell superhydrophilicity |
| title | Effect of Plasma Treatment of Titanium Surface on Biocompatibility |
| title_full | Effect of Plasma Treatment of Titanium Surface on Biocompatibility |
| title_fullStr | Effect of Plasma Treatment of Titanium Surface on Biocompatibility |
| title_full_unstemmed | Effect of Plasma Treatment of Titanium Surface on Biocompatibility |
| title_short | Effect of Plasma Treatment of Titanium Surface on Biocompatibility |
| title_sort | effect of plasma treatment of titanium surface on biocompatibility |
| topic | osseointegration piezobrush rat bone marrow cell superhydrophilicity |
| url | https://www.mdpi.com/2076-3417/9/11/2257 |
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