Modulation of formalin-induced Fos-like immunoreactivity in the spinal cord by swim stress-induced analgesia, morphine and ketamine
Induction of c-fos in the spinal cord due to pain is well established. This study aims to look at the effects of acute swim stress on Fos-like immunoreactivity (FLI) induced by formalin and how it is modulated by ketamine and morphine. Acutely-stressed and non-stressed adult male Sprague Dawley rats...
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| Format: | Article |
| Language: | deu |
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German Medical Science GMS Publishing House
2008-06-01
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| Series: | GMS German Medical Science |
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| Online Access: | http://www.egms.de/en/gms/2008-6/000050.shtml |
| _version_ | 1811331771765096448 |
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| author | Asma Hayati, Ahmad Zalina, Ismail Myo, Than Che Badariah, Abdul Aziz Azhar, Ahmad Idris, Long |
| author_facet | Asma Hayati, Ahmad Zalina, Ismail Myo, Than Che Badariah, Abdul Aziz Azhar, Ahmad Idris, Long |
| author_sort | Asma Hayati, Ahmad |
| collection | DOAJ |
| description | Induction of c-fos in the spinal cord due to pain is well established. This study aims to look at the effects of acute swim stress on Fos-like immunoreactivity (FLI) induced by formalin and how it is modulated by ketamine and morphine. Acutely-stressed and non-stressed adult male Sprague Dawley rats were pretreated with intraperitoneal injection of ketamine 5 mg/kg (Ketava, Atlantic Lab), morphine 10 mg/kg (Rhotard, Custom Pharmaceutical), or saline, 5 minutes prior to experimentation. Rats were acutely stressed by swimming for 3 min in 20°C water. Dilute formalin (Formaldehyde, Merck) was injected to the hindpaw and the formalin score recorded. Rats were then sacrificed and spinal cords (L4-L5) removed for immunohistochemical analysis of FLI. Two-way ANOVA showed significant effects of stress, drug and stress-drug interactions in formalin test and FLI. Both morphine and ketamine produced analgesia in the formalin test. In the saline stressed group, FLI was suppressed on the ipsilateral side (p<0.01) but increased on the contralateral side (p<0.01) compared with non-stressed saline. In morphine and ketamine stressed groups, FLI was increased on both ipsilateral and contralateral sides for morphine (ipsilateral: p<0.05; contralateral: p<0.001) and ketamine (ipsilateral: p<0.05, contralateral: p<0.05) compared with their corresponding non-stressed groups. In conclusion, presence of stress may lead to discrepancy between behavioural manifestation of pain and c-fos induction in the spinal cord. |
| first_indexed | 2024-04-13T16:26:45Z |
| format | Article |
| id | doaj.art-5942b59b16054cb99bd94f06e8ccdfe4 |
| institution | Directory Open Access Journal |
| issn | 1612-3174 |
| language | deu |
| last_indexed | 2024-04-13T16:26:45Z |
| publishDate | 2008-06-01 |
| publisher | German Medical Science GMS Publishing House |
| record_format | Article |
| series | GMS German Medical Science |
| spelling | doaj.art-5942b59b16054cb99bd94f06e8ccdfe42022-12-22T02:39:44ZdeuGerman Medical Science GMS Publishing HouseGMS German Medical Science1612-31742008-06-016Doc05Modulation of formalin-induced Fos-like immunoreactivity in the spinal cord by swim stress-induced analgesia, morphine and ketamineAsma Hayati, AhmadZalina, IsmailMyo, ThanChe Badariah, Abdul AzizAzhar, AhmadIdris, LongInduction of c-fos in the spinal cord due to pain is well established. This study aims to look at the effects of acute swim stress on Fos-like immunoreactivity (FLI) induced by formalin and how it is modulated by ketamine and morphine. Acutely-stressed and non-stressed adult male Sprague Dawley rats were pretreated with intraperitoneal injection of ketamine 5 mg/kg (Ketava, Atlantic Lab), morphine 10 mg/kg (Rhotard, Custom Pharmaceutical), or saline, 5 minutes prior to experimentation. Rats were acutely stressed by swimming for 3 min in 20°C water. Dilute formalin (Formaldehyde, Merck) was injected to the hindpaw and the formalin score recorded. Rats were then sacrificed and spinal cords (L4-L5) removed for immunohistochemical analysis of FLI. Two-way ANOVA showed significant effects of stress, drug and stress-drug interactions in formalin test and FLI. Both morphine and ketamine produced analgesia in the formalin test. In the saline stressed group, FLI was suppressed on the ipsilateral side (p<0.01) but increased on the contralateral side (p<0.01) compared with non-stressed saline. In morphine and ketamine stressed groups, FLI was increased on both ipsilateral and contralateral sides for morphine (ipsilateral: p<0.05; contralateral: p<0.001) and ketamine (ipsilateral: p<0.05, contralateral: p<0.05) compared with their corresponding non-stressed groups. In conclusion, presence of stress may lead to discrepancy between behavioural manifestation of pain and c-fos induction in the spinal cord.http://www.egms.de/en/gms/2008-6/000050.shtmlFosstresspainformalinmorphineketamine |
| spellingShingle | Asma Hayati, Ahmad Zalina, Ismail Myo, Than Che Badariah, Abdul Aziz Azhar, Ahmad Idris, Long Modulation of formalin-induced Fos-like immunoreactivity in the spinal cord by swim stress-induced analgesia, morphine and ketamine GMS German Medical Science Fos stress pain formalin morphine ketamine |
| title | Modulation of formalin-induced Fos-like immunoreactivity in the spinal cord by swim stress-induced analgesia, morphine and ketamine |
| title_full | Modulation of formalin-induced Fos-like immunoreactivity in the spinal cord by swim stress-induced analgesia, morphine and ketamine |
| title_fullStr | Modulation of formalin-induced Fos-like immunoreactivity in the spinal cord by swim stress-induced analgesia, morphine and ketamine |
| title_full_unstemmed | Modulation of formalin-induced Fos-like immunoreactivity in the spinal cord by swim stress-induced analgesia, morphine and ketamine |
| title_short | Modulation of formalin-induced Fos-like immunoreactivity in the spinal cord by swim stress-induced analgesia, morphine and ketamine |
| title_sort | modulation of formalin induced fos like immunoreactivity in the spinal cord by swim stress induced analgesia morphine and ketamine |
| topic | Fos stress pain formalin morphine ketamine |
| url | http://www.egms.de/en/gms/2008-6/000050.shtml |
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