Rapid semi-automated quantitative multiplex tandem PCR (MT-PCR) assays for the differential diagnosis of influenza-like illness
<p>Abstract</p> <p>Background</p> <p>Influenza A, including avian influenza, is a major public health threat in developed and developing countries. Rapid and accurate detection is a key component of strategies to contain spread of infection, and the efficient diagnosis...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-05-01
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| Series: | BMC Infectious Diseases |
| Online Access: | http://www.biomedcentral.com/1471-2334/10/113 |
| _version_ | 1811284114322489344 |
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| author | Dwyer Dominic E Higgins Geoff McPhie Kenneth Anninos Terry Thapa Kiran Szewczuk Elektra Stanley Keith K Iredell Jonathan R |
| author_facet | Dwyer Dominic E Higgins Geoff McPhie Kenneth Anninos Terry Thapa Kiran Szewczuk Elektra Stanley Keith K Iredell Jonathan R |
| author_sort | Dwyer Dominic E |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Influenza A, including avian influenza, is a major public health threat in developed and developing countries. Rapid and accurate detection is a key component of strategies to contain spread of infection, and the efficient diagnosis of influenza-like-illness is essential to protect health infrastructure in the event of a major influenza outbreak.</p> <p>Methods</p> <p>We developed a multiplexed PCR (MT-PCR) assay for the simultaneous diagnosis of respiratory viruses causing influenza-like illness, including the specific recognition of influenza A haemagglutinin subtypes H1, H3, and H5. We tested several hundred clinical specimens in two diagnostic reference laboratories and compared the results with standard techniques.</p> <p>Results</p> <p>The sensitivity and specificity of these assays was higher than individual assays based on direct antigen detection and standard PCR against a range of control templates and in several hundred clinical specimens. The MT-PCR assays provided differential diagnoses as well as potentially useful quantitation of virus in clinical samples.</p> <p>Conclusions</p> <p>MT-PCR is a potentially powerful tool for the differential diagnosis of influenza-like illness in the clinical diagnostic laboratory.</p> |
| first_indexed | 2024-04-13T02:23:45Z |
| format | Article |
| id | doaj.art-5943053aab9545a28fba4a1d71e0d9de |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-04-13T02:23:45Z |
| publishDate | 2010-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-5943053aab9545a28fba4a1d71e0d9de2022-12-22T03:06:50ZengBMCBMC Infectious Diseases1471-23342010-05-0110111310.1186/1471-2334-10-113Rapid semi-automated quantitative multiplex tandem PCR (MT-PCR) assays for the differential diagnosis of influenza-like illnessDwyer Dominic EHiggins GeoffMcPhie KennethAnninos TerryThapa KiranSzewczuk ElektraStanley Keith KIredell Jonathan R<p>Abstract</p> <p>Background</p> <p>Influenza A, including avian influenza, is a major public health threat in developed and developing countries. Rapid and accurate detection is a key component of strategies to contain spread of infection, and the efficient diagnosis of influenza-like-illness is essential to protect health infrastructure in the event of a major influenza outbreak.</p> <p>Methods</p> <p>We developed a multiplexed PCR (MT-PCR) assay for the simultaneous diagnosis of respiratory viruses causing influenza-like illness, including the specific recognition of influenza A haemagglutinin subtypes H1, H3, and H5. We tested several hundred clinical specimens in two diagnostic reference laboratories and compared the results with standard techniques.</p> <p>Results</p> <p>The sensitivity and specificity of these assays was higher than individual assays based on direct antigen detection and standard PCR against a range of control templates and in several hundred clinical specimens. The MT-PCR assays provided differential diagnoses as well as potentially useful quantitation of virus in clinical samples.</p> <p>Conclusions</p> <p>MT-PCR is a potentially powerful tool for the differential diagnosis of influenza-like illness in the clinical diagnostic laboratory.</p>http://www.biomedcentral.com/1471-2334/10/113 |
| spellingShingle | Dwyer Dominic E Higgins Geoff McPhie Kenneth Anninos Terry Thapa Kiran Szewczuk Elektra Stanley Keith K Iredell Jonathan R Rapid semi-automated quantitative multiplex tandem PCR (MT-PCR) assays for the differential diagnosis of influenza-like illness BMC Infectious Diseases |
| title | Rapid semi-automated quantitative multiplex tandem PCR (MT-PCR) assays for the differential diagnosis of influenza-like illness |
| title_full | Rapid semi-automated quantitative multiplex tandem PCR (MT-PCR) assays for the differential diagnosis of influenza-like illness |
| title_fullStr | Rapid semi-automated quantitative multiplex tandem PCR (MT-PCR) assays for the differential diagnosis of influenza-like illness |
| title_full_unstemmed | Rapid semi-automated quantitative multiplex tandem PCR (MT-PCR) assays for the differential diagnosis of influenza-like illness |
| title_short | Rapid semi-automated quantitative multiplex tandem PCR (MT-PCR) assays for the differential diagnosis of influenza-like illness |
| title_sort | rapid semi automated quantitative multiplex tandem pcr mt pcr assays for the differential diagnosis of influenza like illness |
| url | http://www.biomedcentral.com/1471-2334/10/113 |
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