Reliable microRNA profiling in routinely processed formalin-fixed paraffin-embedded breast cancer specimens using fluorescence labelled bead technology
<p>Abstract</p> <p>Background</p> <p>During the last years the analysis of microRNA expression patterns has led to completely new insights into cancer biology. Furthermore, these patterns are a very promising tool for the development of new diagnostic and prognostic mar...
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| Format: | Article |
| Language: | English |
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BMC
2008-11-01
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| Series: | BMC Biotechnology |
| Online Access: | http://www.biomedcentral.com/1472-6750/8/90 |
| _version_ | 1811282443023417344 |
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| author | Kreipe Hans Christgen Matthias Hasemeier Britta Lehmann Ulrich |
| author_facet | Kreipe Hans Christgen Matthias Hasemeier Britta Lehmann Ulrich |
| author_sort | Kreipe Hans |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>During the last years the analysis of microRNA expression patterns has led to completely new insights into cancer biology. Furthermore, these patterns are a very promising tool for the development of new diagnostic and prognostic markers. However, most human tumour samples for which long term clinical records are available exist only as formalin-fixed paraffin-embedded specimens. Therefore, the aim of this study was to examine the feasibility of microRNA profiling studies in routinely processed formalin-fixed paraffin-embedded human breast cancer specimens using fluorescence labelled bead technology.</p> <p>Results</p> <p>A statistically highly significant correlation (Spearman r: 0.78 – 0.90, p < 0.0001) was observed for the expression of 319 microRNAs in routinely processed FFPE breast cancer specimens and paired fresh frozen tissue samples (n = 5). Results were confirmed in a larger series analyzing a selection of 10 microRNAs reported to be deregulated in breast cancer (n = 12). The expression pattern of 3 microRNAs was independently validated in this cohort using real-time RT-PCR technology.</p> <p>Conclusion</p> <p>Comprehensive microRNA expression patterns can be reliably derived from routinely processed FFPE breast cancer specimens using fluorescence labelled bead technology.</p> |
| first_indexed | 2024-04-13T01:52:47Z |
| format | Article |
| id | doaj.art-5945ad40afe448b19750e5706d537e16 |
| institution | Directory Open Access Journal |
| issn | 1472-6750 |
| language | English |
| last_indexed | 2024-04-13T01:52:47Z |
| publishDate | 2008-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biotechnology |
| spelling | doaj.art-5945ad40afe448b19750e5706d537e162022-12-22T03:07:50ZengBMCBMC Biotechnology1472-67502008-11-01819010.1186/1472-6750-8-90Reliable microRNA profiling in routinely processed formalin-fixed paraffin-embedded breast cancer specimens using fluorescence labelled bead technologyKreipe HansChristgen MatthiasHasemeier BrittaLehmann Ulrich<p>Abstract</p> <p>Background</p> <p>During the last years the analysis of microRNA expression patterns has led to completely new insights into cancer biology. Furthermore, these patterns are a very promising tool for the development of new diagnostic and prognostic markers. However, most human tumour samples for which long term clinical records are available exist only as formalin-fixed paraffin-embedded specimens. Therefore, the aim of this study was to examine the feasibility of microRNA profiling studies in routinely processed formalin-fixed paraffin-embedded human breast cancer specimens using fluorescence labelled bead technology.</p> <p>Results</p> <p>A statistically highly significant correlation (Spearman r: 0.78 – 0.90, p < 0.0001) was observed for the expression of 319 microRNAs in routinely processed FFPE breast cancer specimens and paired fresh frozen tissue samples (n = 5). Results were confirmed in a larger series analyzing a selection of 10 microRNAs reported to be deregulated in breast cancer (n = 12). The expression pattern of 3 microRNAs was independently validated in this cohort using real-time RT-PCR technology.</p> <p>Conclusion</p> <p>Comprehensive microRNA expression patterns can be reliably derived from routinely processed FFPE breast cancer specimens using fluorescence labelled bead technology.</p>http://www.biomedcentral.com/1472-6750/8/90 |
| spellingShingle | Kreipe Hans Christgen Matthias Hasemeier Britta Lehmann Ulrich Reliable microRNA profiling in routinely processed formalin-fixed paraffin-embedded breast cancer specimens using fluorescence labelled bead technology BMC Biotechnology |
| title | Reliable microRNA profiling in routinely processed formalin-fixed paraffin-embedded breast cancer specimens using fluorescence labelled bead technology |
| title_full | Reliable microRNA profiling in routinely processed formalin-fixed paraffin-embedded breast cancer specimens using fluorescence labelled bead technology |
| title_fullStr | Reliable microRNA profiling in routinely processed formalin-fixed paraffin-embedded breast cancer specimens using fluorescence labelled bead technology |
| title_full_unstemmed | Reliable microRNA profiling in routinely processed formalin-fixed paraffin-embedded breast cancer specimens using fluorescence labelled bead technology |
| title_short | Reliable microRNA profiling in routinely processed formalin-fixed paraffin-embedded breast cancer specimens using fluorescence labelled bead technology |
| title_sort | reliable microrna profiling in routinely processed formalin fixed paraffin embedded breast cancer specimens using fluorescence labelled bead technology |
| url | http://www.biomedcentral.com/1472-6750/8/90 |
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