The Potential Effect of Polysaccharides Extracted from Red Alga <i>Gelidium spinosum</i> against Intestinal Epithelial Cell Apoptosis
Gut injury is a severe and unpredictable illness related to the increased cell death of intestinal epithelial cells (IECs). Excessive IEC apoptotic cell death during the pathophysiological state entails chronic inflammatory diseases. This investigation was undertaken to assess the cytoprotective act...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/16/3/444 |
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| author | Marwa Ajala Mickael Droguet Marwa Kraiem Hajer Ben Saad Zakaria Boujhoud Abderraouf Hilali Hatem Kallel Jean Marc Pujo Ibtissem Ben Amara |
| author_facet | Marwa Ajala Mickael Droguet Marwa Kraiem Hajer Ben Saad Zakaria Boujhoud Abderraouf Hilali Hatem Kallel Jean Marc Pujo Ibtissem Ben Amara |
| author_sort | Marwa Ajala |
| collection | DOAJ |
| description | Gut injury is a severe and unpredictable illness related to the increased cell death of intestinal epithelial cells (IECs). Excessive IEC apoptotic cell death during the pathophysiological state entails chronic inflammatory diseases. This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, <i>Gelidium spinosum</i> (PSGS), on H<sub>2</sub>O<sub>2</sub>-induced toxicity in IEC-6 cells. The cell viability test was initially carried out to screen out convenient concentrations of H<sub>2</sub>O<sub>2</sub> and PSGS. Subsequently, cells were exposed to 40 µM H<sub>2</sub>O<sub>2</sub> over 4 h in the presence or absence of PSGS. Findings revealed that H<sub>2</sub>O<sub>2</sub> caused oxidative stress manifested by over 70% cell mortality, disturbed the antioxidant defense, and increased the apoptotic rate in IEC-6 cells (32% than normal cells). Pretreatment of PSGS restored cell viability, especially when used at 150 µg/mL and normal cell morphology in H<sub>2</sub>O<sub>2</sub>-callenged cells. PSGS also equally sustained superoxide dismutase and catalase activities and hindered the apoptosis induced by H<sub>2</sub>O<sub>2</sub>. This protection mechanism of PSGS may be associated with its structural composition. The ultraviolet visible spectrum, Fourier-transformed infrared (FT-IR), X-ray diffraction (XRD), and high-performance liquid chromatography (HPLC) demonstrated that PSGS is mainly sulfated polysaccharides. Eventually, this research work provides a deeper insight into the protective functions and enhances the investment of natural resources in handling intestinal diseases. |
| first_indexed | 2024-03-11T06:02:08Z |
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| id | doaj.art-5949c3cbd9da47fa8e713be3ceee4972 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-11T06:02:08Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-5949c3cbd9da47fa8e713be3ceee49722023-11-17T13:12:54ZengMDPI AGPharmaceuticals1424-82472023-03-0116344410.3390/ph16030444The Potential Effect of Polysaccharides Extracted from Red Alga <i>Gelidium spinosum</i> against Intestinal Epithelial Cell ApoptosisMarwa Ajala0Mickael Droguet1Marwa Kraiem2Hajer Ben Saad3Zakaria Boujhoud4Abderraouf Hilali5Hatem Kallel6Jean Marc Pujo7Ibtissem Ben Amara8Laboratory of Medicinal and Environment Chemistry, Higher Institute of Biotechnology, University of Sfax, Sfax 3029, TunisiaORPHY, Optimization of Physiological Regulation, Faculty of Medicine and Health Sciences, 29238 Brest, FranceLaboratory of Medicinal and Environment Chemistry, Higher Institute of Biotechnology, University of Sfax, Sfax 3029, TunisiaLaboratory of Medicinal and Environment Chemistry, Higher Institute of Biotechnology, University of Sfax, Sfax 3029, TunisiaLaboratory of Health Sciences and Technologies, High Institute of Health Sciences, Hassen University, Casablanca 20000, MoroccoLaboratory of Health Sciences and Technologies, High Institute of Health Sciences, Hassen University, Casablanca 20000, MoroccoIntensive Care Unit, Cayenne General Hospital, Cayenne 97300, French GuianaEmergency Department, Cayenne General Hospital, Cayenne 97300, French GuianaLaboratory of Medicinal and Environment Chemistry, Higher Institute of Biotechnology, University of Sfax, Sfax 3029, TunisiaGut injury is a severe and unpredictable illness related to the increased cell death of intestinal epithelial cells (IECs). Excessive IEC apoptotic cell death during the pathophysiological state entails chronic inflammatory diseases. This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, <i>Gelidium spinosum</i> (PSGS), on H<sub>2</sub>O<sub>2</sub>-induced toxicity in IEC-6 cells. The cell viability test was initially carried out to screen out convenient concentrations of H<sub>2</sub>O<sub>2</sub> and PSGS. Subsequently, cells were exposed to 40 µM H<sub>2</sub>O<sub>2</sub> over 4 h in the presence or absence of PSGS. Findings revealed that H<sub>2</sub>O<sub>2</sub> caused oxidative stress manifested by over 70% cell mortality, disturbed the antioxidant defense, and increased the apoptotic rate in IEC-6 cells (32% than normal cells). Pretreatment of PSGS restored cell viability, especially when used at 150 µg/mL and normal cell morphology in H<sub>2</sub>O<sub>2</sub>-callenged cells. PSGS also equally sustained superoxide dismutase and catalase activities and hindered the apoptosis induced by H<sub>2</sub>O<sub>2</sub>. This protection mechanism of PSGS may be associated with its structural composition. The ultraviolet visible spectrum, Fourier-transformed infrared (FT-IR), X-ray diffraction (XRD), and high-performance liquid chromatography (HPLC) demonstrated that PSGS is mainly sulfated polysaccharides. Eventually, this research work provides a deeper insight into the protective functions and enhances the investment of natural resources in handling intestinal diseases.https://www.mdpi.com/1424-8247/16/3/444intestinal epithelial cellsapoptosis<i>Gelidium spinosum</i>antioxidantpolysaccharides |
| spellingShingle | Marwa Ajala Mickael Droguet Marwa Kraiem Hajer Ben Saad Zakaria Boujhoud Abderraouf Hilali Hatem Kallel Jean Marc Pujo Ibtissem Ben Amara The Potential Effect of Polysaccharides Extracted from Red Alga <i>Gelidium spinosum</i> against Intestinal Epithelial Cell Apoptosis Pharmaceuticals intestinal epithelial cells apoptosis <i>Gelidium spinosum</i> antioxidant polysaccharides |
| title | The Potential Effect of Polysaccharides Extracted from Red Alga <i>Gelidium spinosum</i> against Intestinal Epithelial Cell Apoptosis |
| title_full | The Potential Effect of Polysaccharides Extracted from Red Alga <i>Gelidium spinosum</i> against Intestinal Epithelial Cell Apoptosis |
| title_fullStr | The Potential Effect of Polysaccharides Extracted from Red Alga <i>Gelidium spinosum</i> against Intestinal Epithelial Cell Apoptosis |
| title_full_unstemmed | The Potential Effect of Polysaccharides Extracted from Red Alga <i>Gelidium spinosum</i> against Intestinal Epithelial Cell Apoptosis |
| title_short | The Potential Effect of Polysaccharides Extracted from Red Alga <i>Gelidium spinosum</i> against Intestinal Epithelial Cell Apoptosis |
| title_sort | potential effect of polysaccharides extracted from red alga i gelidium spinosum i against intestinal epithelial cell apoptosis |
| topic | intestinal epithelial cells apoptosis <i>Gelidium spinosum</i> antioxidant polysaccharides |
| url | https://www.mdpi.com/1424-8247/16/3/444 |
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