Plasmid DNA damage induced by singlet molecular oxygen released from the naphthalene endoperoxide DHPNO2 and photoactivated methylene blue
To investigate oxidative lesions and strand breaks induction by singlet molecular oxygen (¹O2), supercoiled-DNA plasmid was treated with thermo-dissociated DHPNO2 and photoactivated-methylene blue. DNA lesions were detected by Fpg that cleaves DNA at certain oxidized bases, and T4-endoV, which cleav...
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| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Química
2010-01-01
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| Series: | Química Nova |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000200009 |
| _version_ | 1811328135034044416 |
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| author | Carolina Maria Berra Carlos Frederico Martins Menck Glaucia Regina Martinez Carla Santos de Oliveira Maurício da Silva Baptista Paolo Di Mascio |
| author_facet | Carolina Maria Berra Carlos Frederico Martins Menck Glaucia Regina Martinez Carla Santos de Oliveira Maurício da Silva Baptista Paolo Di Mascio |
| author_sort | Carolina Maria Berra |
| collection | DOAJ |
| description | To investigate oxidative lesions and strand breaks induction by singlet molecular oxygen (¹O2), supercoiled-DNA plasmid was treated with thermo-dissociated DHPNO2 and photoactivated-methylene blue. DNA lesions were detected by Fpg that cleaves DNA at certain oxidized bases, and T4-endoV, which cleaves DNA at cyclobutane pyrimidine dimers and apurinic/apyrimidinic (AP) sites. These cleavages form open relaxed-DNA structures, which are discriminated from supercoiled-DNA. DHPNO2 or photoactivated-MB treatments result in similar plasmid damage profile: low number of single-strand breaks or AP-sites and high frequency of Fpg-sensitive sites; confirming that base oxidation is the main product for both reactions and that ¹O2 might be the most likely intermediate that reacts with DNA. |
| first_indexed | 2024-04-13T15:21:04Z |
| format | Article |
| id | doaj.art-594ddb30643142b1af629087687a60a5 |
| institution | Directory Open Access Journal |
| issn | 0100-4042 1678-7064 |
| language | English |
| last_indexed | 2024-04-13T15:21:04Z |
| publishDate | 2010-01-01 |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Química Nova |
| spelling | doaj.art-594ddb30643142b1af629087687a60a52022-12-22T02:41:41ZengSociedade Brasileira de QuímicaQuímica Nova0100-40421678-70642010-01-0133227928310.1590/S0100-40422010000200009Plasmid DNA damage induced by singlet molecular oxygen released from the naphthalene endoperoxide DHPNO2 and photoactivated methylene blueCarolina Maria BerraCarlos Frederico Martins MenckGlaucia Regina MartinezCarla Santos de OliveiraMaurício da Silva BaptistaPaolo Di MascioTo investigate oxidative lesions and strand breaks induction by singlet molecular oxygen (¹O2), supercoiled-DNA plasmid was treated with thermo-dissociated DHPNO2 and photoactivated-methylene blue. DNA lesions were detected by Fpg that cleaves DNA at certain oxidized bases, and T4-endoV, which cleaves DNA at cyclobutane pyrimidine dimers and apurinic/apyrimidinic (AP) sites. These cleavages form open relaxed-DNA structures, which are discriminated from supercoiled-DNA. DHPNO2 or photoactivated-MB treatments result in similar plasmid damage profile: low number of single-strand breaks or AP-sites and high frequency of Fpg-sensitive sites; confirming that base oxidation is the main product for both reactions and that ¹O2 might be the most likely intermediate that reacts with DNA.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000200009oxidative stressDNA lesionssinglet molecular oxygen |
| spellingShingle | Carolina Maria Berra Carlos Frederico Martins Menck Glaucia Regina Martinez Carla Santos de Oliveira Maurício da Silva Baptista Paolo Di Mascio Plasmid DNA damage induced by singlet molecular oxygen released from the naphthalene endoperoxide DHPNO2 and photoactivated methylene blue Química Nova oxidative stress DNA lesions singlet molecular oxygen |
| title | Plasmid DNA damage induced by singlet molecular oxygen released from the naphthalene endoperoxide DHPNO2 and photoactivated methylene blue |
| title_full | Plasmid DNA damage induced by singlet molecular oxygen released from the naphthalene endoperoxide DHPNO2 and photoactivated methylene blue |
| title_fullStr | Plasmid DNA damage induced by singlet molecular oxygen released from the naphthalene endoperoxide DHPNO2 and photoactivated methylene blue |
| title_full_unstemmed | Plasmid DNA damage induced by singlet molecular oxygen released from the naphthalene endoperoxide DHPNO2 and photoactivated methylene blue |
| title_short | Plasmid DNA damage induced by singlet molecular oxygen released from the naphthalene endoperoxide DHPNO2 and photoactivated methylene blue |
| title_sort | plasmid dna damage induced by singlet molecular oxygen released from the naphthalene endoperoxide dhpno2 and photoactivated methylene blue |
| topic | oxidative stress DNA lesions singlet molecular oxygen |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000200009 |
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