Resistance of <i>Rhodococcus ruber</i> biofilms to CuO nanoparticles depending on exopolymer matrix composition
Background. The widespread use of copper oxide nanoparticles (CuO NPs) increases their release into the environment, which leads to accumulation in trophic chains. Bacterial biofilms are more resistant to physico-chemical factors compared to planktonic cells due to an exopolymer matrix (EPM) consist...
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| Format: | Article |
| Language: | Russian |
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Scientific Сentre for Family Health and Human Reproduction Problems
2022-12-01
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| Series: | Acta Biomedica Scientifica |
| Subjects: | |
| Online Access: | https://www.actabiomedica.ru/jour/article/view/3808 |
| _version_ | 1797237131199905792 |
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| author | E. A. Bayandina G. G. Glebov M. S. Kuyukina I. B. Ivshina |
| author_facet | E. A. Bayandina G. G. Glebov M. S. Kuyukina I. B. Ivshina |
| author_sort | E. A. Bayandina |
| collection | DOAJ |
| description | Background. The widespread use of copper oxide nanoparticles (CuO NPs) increases their release into the environment, which leads to accumulation in trophic chains. Bacterial biofilms are more resistant to physico-chemical factors compared to planktonic cells due to an exopolymer matrix (EPM) consisting of polysaccharides, proteins, lipids and nucleic acids. Rhodococcus actinobacteria are promising for environmental biotechnology due to biodegradation of petroleum products, pesticides and other organic pollutants, as well as bioaccumulation of heavy metals. The aim. To investigate effects of CuO NPs on the viability of Rhodococcus ruber IEGM 231 cells in biofilms and the dynamics of EPM components. Methods. R. ruber biofilms were grown on microscopy cover glass with CuO NPs and EPM components were studied using confocal laser scanning microscopy (CLSM) by differentiating staining with LIVE/DEAD to determine the number of living and dead cells, Nile Red for lipids, FITC for proteins and Calcofluor White for betapolysaccharides. Results. It was found that R. ruber biofilms grown in a mineral medium with1.0 vol.% n-hexadecane are more resistant to CuO NPs compared to biofilms growing in a rich culture medium (meat-peptone broth). This was due to more intensive EPM formation, which plays a major role in protecting cells from the bactericidal action of nanometals. A weak stimulating effect of a low (0.001 g/l) concentration of CuO NPs on biofilm formation was registered. Dynamics and localization of main EPM components were monitored during prolonged (24–72 h) biofilm cultivation with CuO NPs. When exposed to high (0.01–0.1 g/l) concentrations of CuO NPs, a consistently high lipid content and an increase in concentrations of polysaccharides and proteins were revealed. Conclusion. Understanding the complex interaction mechanisms of nanometals and biofilms will contribute to the development of effective biocatalysts based on immobilized bacterial cells. Also, the obtained data can be used to combat unwanted biofilms with the help of metal nanoparticles. |
| first_indexed | 2024-03-08T12:15:52Z |
| format | Article |
| id | doaj.art-59500d5d5a47404fa36be12ac10a2493 |
| institution | Directory Open Access Journal |
| issn | 2541-9420 2587-9596 |
| language | Russian |
| last_indexed | 2024-04-24T17:14:52Z |
| publishDate | 2022-12-01 |
| publisher | Scientific Сentre for Family Health and Human Reproduction Problems |
| record_format | Article |
| series | Acta Biomedica Scientifica |
| spelling | doaj.art-59500d5d5a47404fa36be12ac10a24932024-03-28T07:49:27ZrusScientific Сentre for Family Health and Human Reproduction ProblemsActa Biomedica Scientifica2541-94202587-95962022-12-0175-110010910.29413/ABS.2022-7.5-1.112422Resistance of <i>Rhodococcus ruber</i> biofilms to CuO nanoparticles depending on exopolymer matrix compositionE. A. Bayandina0G. G. Glebov1M. S. Kuyukina2I. B. Ivshina3Perm State UniversityPerm State University; Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center of the Ural Branch of the Russian Academy of SciencesPerm State University; Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center of the Ural Branch of the Russian Academy of SciencesPerm State University; Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center of the Ural Branch of the Russian Academy of SciencesBackground. The widespread use of copper oxide nanoparticles (CuO NPs) increases their release into the environment, which leads to accumulation in trophic chains. Bacterial biofilms are more resistant to physico-chemical factors compared to planktonic cells due to an exopolymer matrix (EPM) consisting of polysaccharides, proteins, lipids and nucleic acids. Rhodococcus actinobacteria are promising for environmental biotechnology due to biodegradation of petroleum products, pesticides and other organic pollutants, as well as bioaccumulation of heavy metals. The aim. To investigate effects of CuO NPs on the viability of Rhodococcus ruber IEGM 231 cells in biofilms and the dynamics of EPM components. Methods. R. ruber biofilms were grown on microscopy cover glass with CuO NPs and EPM components were studied using confocal laser scanning microscopy (CLSM) by differentiating staining with LIVE/DEAD to determine the number of living and dead cells, Nile Red for lipids, FITC for proteins and Calcofluor White for betapolysaccharides. Results. It was found that R. ruber biofilms grown in a mineral medium with1.0 vol.% n-hexadecane are more resistant to CuO NPs compared to biofilms growing in a rich culture medium (meat-peptone broth). This was due to more intensive EPM formation, which plays a major role in protecting cells from the bactericidal action of nanometals. A weak stimulating effect of a low (0.001 g/l) concentration of CuO NPs on biofilm formation was registered. Dynamics and localization of main EPM components were monitored during prolonged (24–72 h) biofilm cultivation with CuO NPs. When exposed to high (0.01–0.1 g/l) concentrations of CuO NPs, a consistently high lipid content and an increase in concentrations of polysaccharides and proteins were revealed. Conclusion. Understanding the complex interaction mechanisms of nanometals and biofilms will contribute to the development of effective biocatalysts based on immobilized bacterial cells. Also, the obtained data can be used to combat unwanted biofilms with the help of metal nanoparticles.https://www.actabiomedica.ru/jour/article/view/3808biofilmsrhodococcusmetal nanoparticlesconfocal laser scanning microscopyexopolymer matrix |
| spellingShingle | E. A. Bayandina G. G. Glebov M. S. Kuyukina I. B. Ivshina Resistance of <i>Rhodococcus ruber</i> biofilms to CuO nanoparticles depending on exopolymer matrix composition Acta Biomedica Scientifica biofilms rhodococcus metal nanoparticles confocal laser scanning microscopy exopolymer matrix |
| title | Resistance of <i>Rhodococcus ruber</i> biofilms to CuO nanoparticles depending on exopolymer matrix composition |
| title_full | Resistance of <i>Rhodococcus ruber</i> biofilms to CuO nanoparticles depending on exopolymer matrix composition |
| title_fullStr | Resistance of <i>Rhodococcus ruber</i> biofilms to CuO nanoparticles depending on exopolymer matrix composition |
| title_full_unstemmed | Resistance of <i>Rhodococcus ruber</i> biofilms to CuO nanoparticles depending on exopolymer matrix composition |
| title_short | Resistance of <i>Rhodococcus ruber</i> biofilms to CuO nanoparticles depending on exopolymer matrix composition |
| title_sort | resistance of i rhodococcus ruber i biofilms to cuo nanoparticles depending on exopolymer matrix composition |
| topic | biofilms rhodococcus metal nanoparticles confocal laser scanning microscopy exopolymer matrix |
| url | https://www.actabiomedica.ru/jour/article/view/3808 |
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