Effect of Different Molecular Weights of Polyacrylic Acid on Rat Lung Following Intratracheal Instillation
Background: We conducted intratracheal instillations of different molecular weights of polyacrylic acid (PAA) into rats in order to examine what kinds of physicochemical characteristics of acrylic acid-based polymer affect responses in the lung. Methods: F344 rats were intratracheally exposed to a h...
| Main Authors: | , , , , , , , , , , , , , , |
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/18/10345 |
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| author | Chinatsu Nishida Hiroto Izumi Taisuke Tomonaga Ke-Yong Wang Hidenori Higashi Jun-Ichi Takeshita Ryohei Ono Kazuki Sumiya Shota Fujii Yuki Hata Kazuo Sakurai Yasuyuki Higashi Kei Yamasaki Kazuhiro Yatera Yasuo Morimoto |
| author_facet | Chinatsu Nishida Hiroto Izumi Taisuke Tomonaga Ke-Yong Wang Hidenori Higashi Jun-Ichi Takeshita Ryohei Ono Kazuki Sumiya Shota Fujii Yuki Hata Kazuo Sakurai Yasuyuki Higashi Kei Yamasaki Kazuhiro Yatera Yasuo Morimoto |
| author_sort | Chinatsu Nishida |
| collection | DOAJ |
| description | Background: We conducted intratracheal instillations of different molecular weights of polyacrylic acid (PAA) into rats in order to examine what kinds of physicochemical characteristics of acrylic acid-based polymer affect responses in the lung. Methods: F344 rats were intratracheally exposed to a high molecular weight (HMW) of 598 thousand g/mol or a low molecular weight (LMW) of 30.9 thousand g/mol PAA at low and high doses. Rats were sacrificed at 3 days, 1 week, 1 month, 3 months and 6 months post exposure. Results: HMW PAA caused persistent increases in neutrophil influx, cytokine-induced neutrophil chemoattractants (CINC) in the bronchoalveolar lavage fluid (BALF), and heme oxygenase-1 (HO-1) in the lung tissue from 3 days to 3 months and 6 months following instillation. On the other hand, LMW PAA caused only transient increases in neutrophil influx, CINC in BALF, and HO-1 in the lung tissue from 3 days to up to 1 week or 1 month following instillation. Histopathological findings of the lungs demonstrated that the extensive inflammation and fibrotic changes caused by the HMW PAA was greater than that in exposure to the LMW PAA during the observation period. Conclusion: HMW PAA induced persistence of lung disorder, suggesting that molecular weight is a physicochemical characteristic of PAA-induced lung disorder. |
| first_indexed | 2024-03-09T23:47:39Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:47:39Z |
| publishDate | 2022-09-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5954461a43fe45308ceb34b34bba48092023-11-23T16:40:00ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123181034510.3390/ijms231810345Effect of Different Molecular Weights of Polyacrylic Acid on Rat Lung Following Intratracheal InstillationChinatsu Nishida0Hiroto Izumi1Taisuke Tomonaga2Ke-Yong Wang3Hidenori Higashi4Jun-Ichi Takeshita5Ryohei Ono6Kazuki Sumiya7Shota Fujii8Yuki Hata9Kazuo Sakurai10Yasuyuki Higashi11Kei Yamasaki12Kazuhiro Yatera13Yasuo Morimoto14Department of Respiratory Medicine, University of Occupational and Environmental Health, Japan 1-1 Iseigaoka, Yahata-nishi-ku, Kitakyushu 807-8555, Fukuoka, JapanDepartment of Occupational Pneumology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Japan 1-1 Iseigaoka, Yahata-nishi-ku, Kitakyushu 807-8555, Fukuoka, JapanDepartment of Occupational Pneumology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Japan 1-1 Iseigaoka, Yahata-nishi-ku, Kitakyushu 807-8555, Fukuoka, JapanShared-Use Research Center, School of Medicine, University of Occupational and Environmental Health, Japan 1-1 Iseigaoka, Yahata-nishi-ku, Kitakyushu 807-8555, Fukuoka, JapanDepartment of Environmental Health Engineering, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Japan 1-1 Iseigaoka, Yahata-nishi-ku, Kitakyushu 807-8555, Fukuoka, JapanResearch Institute of Science for Safety and Sustainability, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan 16-1 Onogawa, Tsukuba 305-8569, Ibaraki, JapanDepartment of Chemistry and Biochemistry, The University of Kitakyushu, 1-1, Hibikino, Wakamatsu-ku, Kitakyushu 808-0135, Fukuoka, JapanDepartment of Chemistry and Biochemistry, The University of Kitakyushu, 1-1, Hibikino, Wakamatsu-ku, Kitakyushu 808-0135, Fukuoka, JapanDepartment of Chemistry and Biochemistry, The University of Kitakyushu, 1-1, Hibikino, Wakamatsu-ku, Kitakyushu 808-0135, Fukuoka, JapanDepartment of Chemistry and Biochemistry, The University of Kitakyushu, 1-1, Hibikino, Wakamatsu-ku, Kitakyushu 808-0135, Fukuoka, JapanDepartment of Chemistry and Biochemistry, The University of Kitakyushu, 1-1, Hibikino, Wakamatsu-ku, Kitakyushu 808-0135, Fukuoka, JapanDepartment of Respiratory Medicine, University of Occupational and Environmental Health, Japan 1-1 Iseigaoka, Yahata-nishi-ku, Kitakyushu 807-8555, Fukuoka, JapanDepartment of Respiratory Medicine, University of Occupational and Environmental Health, Japan 1-1 Iseigaoka, Yahata-nishi-ku, Kitakyushu 807-8555, Fukuoka, JapanDepartment of Respiratory Medicine, University of Occupational and Environmental Health, Japan 1-1 Iseigaoka, Yahata-nishi-ku, Kitakyushu 807-8555, Fukuoka, JapanDepartment of Occupational Pneumology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Japan 1-1 Iseigaoka, Yahata-nishi-ku, Kitakyushu 807-8555, Fukuoka, JapanBackground: We conducted intratracheal instillations of different molecular weights of polyacrylic acid (PAA) into rats in order to examine what kinds of physicochemical characteristics of acrylic acid-based polymer affect responses in the lung. Methods: F344 rats were intratracheally exposed to a high molecular weight (HMW) of 598 thousand g/mol or a low molecular weight (LMW) of 30.9 thousand g/mol PAA at low and high doses. Rats were sacrificed at 3 days, 1 week, 1 month, 3 months and 6 months post exposure. Results: HMW PAA caused persistent increases in neutrophil influx, cytokine-induced neutrophil chemoattractants (CINC) in the bronchoalveolar lavage fluid (BALF), and heme oxygenase-1 (HO-1) in the lung tissue from 3 days to 3 months and 6 months following instillation. On the other hand, LMW PAA caused only transient increases in neutrophil influx, CINC in BALF, and HO-1 in the lung tissue from 3 days to up to 1 week or 1 month following instillation. Histopathological findings of the lungs demonstrated that the extensive inflammation and fibrotic changes caused by the HMW PAA was greater than that in exposure to the LMW PAA during the observation period. Conclusion: HMW PAA induced persistence of lung disorder, suggesting that molecular weight is a physicochemical characteristic of PAA-induced lung disorder.https://www.mdpi.com/1422-0067/23/18/10345polyacrylic acid (PAA)organic compoundsmolecular weightpulmonary toxicity |
| spellingShingle | Chinatsu Nishida Hiroto Izumi Taisuke Tomonaga Ke-Yong Wang Hidenori Higashi Jun-Ichi Takeshita Ryohei Ono Kazuki Sumiya Shota Fujii Yuki Hata Kazuo Sakurai Yasuyuki Higashi Kei Yamasaki Kazuhiro Yatera Yasuo Morimoto Effect of Different Molecular Weights of Polyacrylic Acid on Rat Lung Following Intratracheal Instillation International Journal of Molecular Sciences polyacrylic acid (PAA) organic compounds molecular weight pulmonary toxicity |
| title | Effect of Different Molecular Weights of Polyacrylic Acid on Rat Lung Following Intratracheal Instillation |
| title_full | Effect of Different Molecular Weights of Polyacrylic Acid on Rat Lung Following Intratracheal Instillation |
| title_fullStr | Effect of Different Molecular Weights of Polyacrylic Acid on Rat Lung Following Intratracheal Instillation |
| title_full_unstemmed | Effect of Different Molecular Weights of Polyacrylic Acid on Rat Lung Following Intratracheal Instillation |
| title_short | Effect of Different Molecular Weights of Polyacrylic Acid on Rat Lung Following Intratracheal Instillation |
| title_sort | effect of different molecular weights of polyacrylic acid on rat lung following intratracheal instillation |
| topic | polyacrylic acid (PAA) organic compounds molecular weight pulmonary toxicity |
| url | https://www.mdpi.com/1422-0067/23/18/10345 |
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