Antibacterial and Antioxidant Compounds from Root Extracts of Gloriosa superba Linn: A Combined Experimental and Computational Study
Throughout history, medicinal plants have globally served as remedies for various ailments, and diseases. The roots of Gloriosa superba are traditionally used to treat antitumor, antimicrobial, and anti-inflammatory diseases. In this study, the roots of G. superba (320 g) were successively extracte...
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| Format: | Article |
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Adama Science and Technology University
2024-02-01
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| Series: | Ethiopian Journal of Science and Sustainable Development |
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| Online Access: | http://213.55.101.19/index.php/EJSSD/article/view/725 |
| _version_ | 1827352637178118144 |
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| author | Iman Mustefa Milkyas Endale Rajalakshmanan Eswaramoorthy |
| author_facet | Iman Mustefa Milkyas Endale Rajalakshmanan Eswaramoorthy |
| author_sort | Iman Mustefa |
| collection | DOAJ |
| description |
Throughout history, medicinal plants have globally served as remedies for various ailments, and diseases. The roots of Gloriosa superba are traditionally used to treat antitumor, antimicrobial, and anti-inflammatory diseases. In this study, the roots of G. superba (320 g) were successively extracted with n-hexane, chloroform, and methanol to afford 530 mg (0.17%), 2.89 g (0.90%), and 17.78 g (5.56%) yields, respectively. Silica gel column chromatographic separation of the combined chloroform and methanol extracts gave 4-methoxy caffeic acid heptyl ester (1), desmosterol (2), 3-hydroxymethyl phenol (3), 3-Hydroxy-5-methoxy-benzoic acid (4), sucrose (5) and rutinose (6). In vitro antibacterial study revealed promising zone of inhibition value by chloroform extract against Klebsiella pneumoniae (13±0.00 mm) compared to gentamicin (15.86±4.67 mm). Desmosterol (2), 3-hydroxymethyl phenol (3), and 3-Hydroxy-5-methoxy-benzoic acid (4) displayed promising zone of inhibition against K. pneumonia (12.33±0.58, 11.33±1.53 and 11.33±1.15 mm, respectively) at 1000 μg/mL compared to gentamycin (15.86±4.67 mm at 100 μg/mL). Promising inhibition zone values were also displayed by desmosterol (2) and 3-Hydroxy-5-methoxy-benzoic acid (4) against Pseudomonas aeruginosa (14±1.00 and 14±1.73 mm, respectively) compared to gentamycin (25±2.52 mm).Chloroform extract displayed 95.14% DPPH radical scavenging value compared to ascorbic acid (96.11%) at 200 μg/mL. Compounds 2 and 4 displayed binding affinities of -7.8 and -6.5 Kcal/mol, respectively, against PqsA protein of P. aeruginosa, compared to amoxicillin (-7.3 kcal/mol). Therefore, the in vitro antibacterial and radical scavenging activity results suggest the potential uses of the root extracts of G. superba as promising antibacterial agents and free radical scavengers.
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| first_indexed | 2024-03-08T03:08:52Z |
| format | Article |
| id | doaj.art-5954715de2fe42b8846be88351160ee8 |
| institution | Directory Open Access Journal |
| issn | 1998-0531 2663-3205 |
| language | English |
| last_indexed | 2024-03-08T03:08:52Z |
| publishDate | 2024-02-01 |
| publisher | Adama Science and Technology University |
| record_format | Article |
| series | Ethiopian Journal of Science and Sustainable Development |
| spelling | doaj.art-5954715de2fe42b8846be88351160ee82024-02-13T07:30:01ZengAdama Science and Technology UniversityEthiopian Journal of Science and Sustainable Development1998-05312663-32052024-02-0111110.20372/ejssdastu:v11.i1.2024.725Antibacterial and Antioxidant Compounds from Root Extracts of Gloriosa superba Linn: A Combined Experimental and Computational StudyIman Mustefa0Milkyas Endale1Rajalakshmanan Eswaramoorthy2Adama Science and Technology UniversityTraditional and Modern Medicine Research and Development Directorate, Armauer Hansen Research Institute (AHRI), P.O.Box 1242, Addis Ababa, EthiopiaDepartment of Biomaterials, Saveetha Dental College and Hospital, Saveetha University, Chennai 600 077, India Throughout history, medicinal plants have globally served as remedies for various ailments, and diseases. The roots of Gloriosa superba are traditionally used to treat antitumor, antimicrobial, and anti-inflammatory diseases. In this study, the roots of G. superba (320 g) were successively extracted with n-hexane, chloroform, and methanol to afford 530 mg (0.17%), 2.89 g (0.90%), and 17.78 g (5.56%) yields, respectively. Silica gel column chromatographic separation of the combined chloroform and methanol extracts gave 4-methoxy caffeic acid heptyl ester (1), desmosterol (2), 3-hydroxymethyl phenol (3), 3-Hydroxy-5-methoxy-benzoic acid (4), sucrose (5) and rutinose (6). In vitro antibacterial study revealed promising zone of inhibition value by chloroform extract against Klebsiella pneumoniae (13±0.00 mm) compared to gentamicin (15.86±4.67 mm). Desmosterol (2), 3-hydroxymethyl phenol (3), and 3-Hydroxy-5-methoxy-benzoic acid (4) displayed promising zone of inhibition against K. pneumonia (12.33±0.58, 11.33±1.53 and 11.33±1.15 mm, respectively) at 1000 μg/mL compared to gentamycin (15.86±4.67 mm at 100 μg/mL). Promising inhibition zone values were also displayed by desmosterol (2) and 3-Hydroxy-5-methoxy-benzoic acid (4) against Pseudomonas aeruginosa (14±1.00 and 14±1.73 mm, respectively) compared to gentamycin (25±2.52 mm).Chloroform extract displayed 95.14% DPPH radical scavenging value compared to ascorbic acid (96.11%) at 200 μg/mL. Compounds 2 and 4 displayed binding affinities of -7.8 and -6.5 Kcal/mol, respectively, against PqsA protein of P. aeruginosa, compared to amoxicillin (-7.3 kcal/mol). Therefore, the in vitro antibacterial and radical scavenging activity results suggest the potential uses of the root extracts of G. superba as promising antibacterial agents and free radical scavengers. http://213.55.101.19/index.php/EJSSD/article/view/725AntitumorAntimicrobialAnthelminticAnti-inflammatoryAntioxidant activityGloriosa superba |
| spellingShingle | Iman Mustefa Milkyas Endale Rajalakshmanan Eswaramoorthy Antibacterial and Antioxidant Compounds from Root Extracts of Gloriosa superba Linn: A Combined Experimental and Computational Study Ethiopian Journal of Science and Sustainable Development Antitumor Antimicrobial Anthelmintic Anti-inflammatory Antioxidant activity Gloriosa superba |
| title | Antibacterial and Antioxidant Compounds from Root Extracts of Gloriosa superba Linn: A Combined Experimental and Computational Study |
| title_full | Antibacterial and Antioxidant Compounds from Root Extracts of Gloriosa superba Linn: A Combined Experimental and Computational Study |
| title_fullStr | Antibacterial and Antioxidant Compounds from Root Extracts of Gloriosa superba Linn: A Combined Experimental and Computational Study |
| title_full_unstemmed | Antibacterial and Antioxidant Compounds from Root Extracts of Gloriosa superba Linn: A Combined Experimental and Computational Study |
| title_short | Antibacterial and Antioxidant Compounds from Root Extracts of Gloriosa superba Linn: A Combined Experimental and Computational Study |
| title_sort | antibacterial and antioxidant compounds from root extracts of gloriosa superba linn a combined experimental and computational study |
| topic | Antitumor Antimicrobial Anthelmintic Anti-inflammatory Antioxidant activity Gloriosa superba |
| url | http://213.55.101.19/index.php/EJSSD/article/view/725 |
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