Effect of hypoxia on progesterone production by cultured bovine early and mid luteal cells
A major role of the corpus luteum (CL) is to produce progesterone (P4). The CL has immature vasculature shortly after ovulation, suggesting it exists under hypoxic conditions. To elucidate the mechanism involved in regulation of luteal cell function during CL development, we compared the effect of h...
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| Format: | Article |
| Language: | English |
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The Society for Reproduction and Development
2018-11-01
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| Series: | The Journal of Reproduction and Development |
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| Online Access: | https://www.jstage.jst.go.jp/article/jrd/65/1/65_2018-061/_pdf/-char/en |
| _version_ | 1827752377514459136 |
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| author | Hiroki HASEGAWA Ryo NISHIMURA Masamichi YAMASHITA Takeshi YAMAGUCHI Mitsugu HISHINUMA Kiyoshi OKUDA |
| author_facet | Hiroki HASEGAWA Ryo NISHIMURA Masamichi YAMASHITA Takeshi YAMAGUCHI Mitsugu HISHINUMA Kiyoshi OKUDA |
| author_sort | Hiroki HASEGAWA |
| collection | DOAJ |
| description | A major role of the corpus luteum (CL) is to produce progesterone (P4). The CL has immature vasculature shortly after ovulation, suggesting it exists under hypoxic conditions. To elucidate the mechanism involved in regulation of luteal cell function during CL development, we compared the effect of hypoxia on P4 production by cultured bovine early and mid luteal cells. Luteal cells obtained from early and mid CL were incubated under different O2 concentrations (20% and 3%) with or without hCG (1 U/ml) for 6 h and 24 h. After 6 h of culture in the presence of hCG, P4 production was not affected by hypoxia whereas decrease in its production by mid luteal cells was observed. After 24 h of culture, P4 production was significantly decreased by hypoxia in both stages of luteal cells regardless of the use of hCG. At 6 h of culture, hypoxia increased mRNA expression of hydroxyl-Δ-5-steroid dehydrogenase, 3β- and steroid Δ-isomerase 1 (HSD3B1) in early luteal cells, and decreased mRNA expression of cytochrome P450 cholesterol side chain cleavage (CYP11A1) enzyme in mid luteal cells. At 24 h of culture, mRNA expressions of steroidogenic acute regulatory protein (STAR), CYP11A1, and HSD3B1 were not affected by hypoxia in both stages of luteal cells. The overall results suggest that early luteal cells maintain P4 production under hypoxic conditions, and hypoxia-induced HSD3B1 may support this P4 production in the bovine early CL. |
| first_indexed | 2024-03-11T07:15:28Z |
| format | Article |
| id | doaj.art-59652a23c6194a10984e392bd5990b67 |
| institution | Directory Open Access Journal |
| issn | 0916-8818 1348-4400 |
| language | English |
| last_indexed | 2024-03-11T07:15:28Z |
| publishDate | 2018-11-01 |
| publisher | The Society for Reproduction and Development |
| record_format | Article |
| series | The Journal of Reproduction and Development |
| spelling | doaj.art-59652a23c6194a10984e392bd5990b672023-11-17T08:17:57ZengThe Society for Reproduction and DevelopmentThe Journal of Reproduction and Development0916-88181348-44002018-11-01651677210.1262/jrd.2018-061jrdEffect of hypoxia on progesterone production by cultured bovine early and mid luteal cellsHiroki HASEGAWA0Ryo NISHIMURA1Masamichi YAMASHITA2Takeshi YAMAGUCHI3Mitsugu HISHINUMA4Kiyoshi OKUDA5Laboratory of Reproductive Physiology, Graduate School of Agriculture, Tottori University, Tottori 680-8553, JapanLaboratory of Reproductive Physiology, Graduate School of Agriculture, Tottori University, Tottori 680-8553, JapanJoint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8553, JapanLaboratory of Crop Husbandry, Faculty of Agriculture, Tottori University, Tottori 680-8553, JapanLaboratory of Theriogenology, Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8553, JapanObihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, JapanA major role of the corpus luteum (CL) is to produce progesterone (P4). The CL has immature vasculature shortly after ovulation, suggesting it exists under hypoxic conditions. To elucidate the mechanism involved in regulation of luteal cell function during CL development, we compared the effect of hypoxia on P4 production by cultured bovine early and mid luteal cells. Luteal cells obtained from early and mid CL were incubated under different O2 concentrations (20% and 3%) with or without hCG (1 U/ml) for 6 h and 24 h. After 6 h of culture in the presence of hCG, P4 production was not affected by hypoxia whereas decrease in its production by mid luteal cells was observed. After 24 h of culture, P4 production was significantly decreased by hypoxia in both stages of luteal cells regardless of the use of hCG. At 6 h of culture, hypoxia increased mRNA expression of hydroxyl-Δ-5-steroid dehydrogenase, 3β- and steroid Δ-isomerase 1 (HSD3B1) in early luteal cells, and decreased mRNA expression of cytochrome P450 cholesterol side chain cleavage (CYP11A1) enzyme in mid luteal cells. At 24 h of culture, mRNA expressions of steroidogenic acute regulatory protein (STAR), CYP11A1, and HSD3B1 were not affected by hypoxia in both stages of luteal cells. The overall results suggest that early luteal cells maintain P4 production under hypoxic conditions, and hypoxia-induced HSD3B1 may support this P4 production in the bovine early CL.https://www.jstage.jst.go.jp/article/jrd/65/1/65_2018-061/_pdf/-char/encorpus luteumearly and mid luteal cellshypoxiaprogesterone |
| spellingShingle | Hiroki HASEGAWA Ryo NISHIMURA Masamichi YAMASHITA Takeshi YAMAGUCHI Mitsugu HISHINUMA Kiyoshi OKUDA Effect of hypoxia on progesterone production by cultured bovine early and mid luteal cells The Journal of Reproduction and Development corpus luteum early and mid luteal cells hypoxia progesterone |
| title | Effect of hypoxia on progesterone production by cultured bovine early and mid luteal cells |
| title_full | Effect of hypoxia on progesterone production by cultured bovine early and mid luteal cells |
| title_fullStr | Effect of hypoxia on progesterone production by cultured bovine early and mid luteal cells |
| title_full_unstemmed | Effect of hypoxia on progesterone production by cultured bovine early and mid luteal cells |
| title_short | Effect of hypoxia on progesterone production by cultured bovine early and mid luteal cells |
| title_sort | effect of hypoxia on progesterone production by cultured bovine early and mid luteal cells |
| topic | corpus luteum early and mid luteal cells hypoxia progesterone |
| url | https://www.jstage.jst.go.jp/article/jrd/65/1/65_2018-061/_pdf/-char/en |
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