Overcoming Encouragement of Dragon Fruit Plant () against Stem Brown Spot Disease Caused by Using Combined with Sodium Bicarbonate
The use of the supernatant from a Bacillus subtilis culture mixed with sodium bicarbonate was explored as a means of controlling stem brown spot disease in dragon fruit plants. In in vitro experiments, the B. subtilis supernatant used with sodium bicarbonate showed a strong inhibition effect on the...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hanrimwon Publishing Company
2021-06-01
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| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | http://www.ppjonline.org/upload/pdf/PPJ-OA-01-2021-0007.pdf |
| _version_ | 1811308495481339904 |
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| author | Sanan Ratanaprom Korakot Nakkanong Charassri Nualsri Palakrit Jiwanit Thanyakorn Rongsawat Natthakorn Woraathakorn |
| author_facet | Sanan Ratanaprom Korakot Nakkanong Charassri Nualsri Palakrit Jiwanit Thanyakorn Rongsawat Natthakorn Woraathakorn |
| author_sort | Sanan Ratanaprom |
| collection | DOAJ |
| description | The use of the supernatant from a Bacillus subtilis culture mixed with sodium bicarbonate was explored as a means of controlling stem brown spot disease in dragon fruit plants. In in vitro experiments, the B. subtilis supernatant used with sodium bicarbonate showed a strong inhibition effect on the growth of the fungus, Neoscytalidium dimidiatum, the agent causing stem brown spot disease and was notably effective in preventing fungal invasion of dragon fruit plant. This combination not only directly suppressed the growth of N. dimidiatum, but also indirectly affected the development of the disease by eliciting the dragon-fruit plant’s defense response. Substantial levels of the pathogenesis-related proteins, chitinase and glucanase, and the phenylpropanoid biosynthetic pathway enzymes, peroxidase and phenyl alanine ammonia-lyase, were triggered. Significant lignin deposition was also detected in treated cladodes of injured dragon fruit plants in in vivo experiments. In summary, B. subtilis supernatant combined with sodium bicarbonate protected dragon fruit plant loss through stem brown spot disease during plant development in the field through pathogenic fungal inhibition and the induction of defense response mechanisms. |
| first_indexed | 2024-04-13T09:24:18Z |
| format | Article |
| id | doaj.art-59672eff2b2c4302a4dd28b141a70359 |
| institution | Directory Open Access Journal |
| issn | 1598-2254 2093-9280 |
| language | English |
| last_indexed | 2024-04-13T09:24:18Z |
| publishDate | 2021-06-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-59672eff2b2c4302a4dd28b141a703592022-12-22T02:52:30ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542093-92802021-06-0137320521410.5423/PPJ.OA.01.2021.00072246Overcoming Encouragement of Dragon Fruit Plant () against Stem Brown Spot Disease Caused by Using Combined with Sodium BicarbonateSanan Ratanaprom0Korakot Nakkanong1Charassri Nualsri2Palakrit Jiwanit3Thanyakorn Rongsawat4Natthakorn Woraathakorn5 Faculty of Science and Technology, Prince of Songkla University, Pattani Campus, Pattani 94000, Thailand Agriculture Innovation and Management Division, Faculty of Natural Resources, Prince of Songkla University, Hat Yai 90112, Thailand Agriculture Innovation and Management Division, Faculty of Natural Resources, Prince of Songkla University, Hat Yai 90112, Thailand Faculty of Science and Technology, Prince of Songkla University, Pattani Campus, Pattani 94000, Thailand Tropical Fruit and Plantation Crops Research Center, Faculty of Natural Resources, Prince of Songkla University, Hat Yai 90112, Thailand Faculty of Science and Technology, Prince of Songkla University, Pattani Campus, Pattani 94000, ThailandThe use of the supernatant from a Bacillus subtilis culture mixed with sodium bicarbonate was explored as a means of controlling stem brown spot disease in dragon fruit plants. In in vitro experiments, the B. subtilis supernatant used with sodium bicarbonate showed a strong inhibition effect on the growth of the fungus, Neoscytalidium dimidiatum, the agent causing stem brown spot disease and was notably effective in preventing fungal invasion of dragon fruit plant. This combination not only directly suppressed the growth of N. dimidiatum, but also indirectly affected the development of the disease by eliciting the dragon-fruit plant’s defense response. Substantial levels of the pathogenesis-related proteins, chitinase and glucanase, and the phenylpropanoid biosynthetic pathway enzymes, peroxidase and phenyl alanine ammonia-lyase, were triggered. Significant lignin deposition was also detected in treated cladodes of injured dragon fruit plants in in vivo experiments. In summary, B. subtilis supernatant combined with sodium bicarbonate protected dragon fruit plant loss through stem brown spot disease during plant development in the field through pathogenic fungal inhibition and the induction of defense response mechanisms.http://www.ppjonline.org/upload/pdf/PPJ-OA-01-2021-0007.pdfdragon fruit plantplant defense responsesodium bicarbonatestem brown spot |
| spellingShingle | Sanan Ratanaprom Korakot Nakkanong Charassri Nualsri Palakrit Jiwanit Thanyakorn Rongsawat Natthakorn Woraathakorn Overcoming Encouragement of Dragon Fruit Plant () against Stem Brown Spot Disease Caused by Using Combined with Sodium Bicarbonate The Plant Pathology Journal dragon fruit plant plant defense response sodium bicarbonate stem brown spot |
| title | Overcoming Encouragement of Dragon Fruit Plant () against Stem Brown Spot Disease Caused by Using Combined with Sodium Bicarbonate |
| title_full | Overcoming Encouragement of Dragon Fruit Plant () against Stem Brown Spot Disease Caused by Using Combined with Sodium Bicarbonate |
| title_fullStr | Overcoming Encouragement of Dragon Fruit Plant () against Stem Brown Spot Disease Caused by Using Combined with Sodium Bicarbonate |
| title_full_unstemmed | Overcoming Encouragement of Dragon Fruit Plant () against Stem Brown Spot Disease Caused by Using Combined with Sodium Bicarbonate |
| title_short | Overcoming Encouragement of Dragon Fruit Plant () against Stem Brown Spot Disease Caused by Using Combined with Sodium Bicarbonate |
| title_sort | overcoming encouragement of dragon fruit plant against stem brown spot disease caused by using combined with sodium bicarbonate |
| topic | dragon fruit plant plant defense response sodium bicarbonate stem brown spot |
| url | http://www.ppjonline.org/upload/pdf/PPJ-OA-01-2021-0007.pdf |
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