The Detection of Helicobacter hepaticus Using Whispering-Gallery Mode Microcavity Optical Sensors
Current bacterial detection techniques are relatively slow, require bulky instrumentation, and usually require some form of specialized training. The gold standard for bacterial detection is culture testing, which can take several days to receive a viable result. Therefore, simpler detection techniq...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-08-01
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| Series: | Biosensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2079-6374/5/3/562 |
| _version_ | 1818671084534235136 |
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| author | Mark E. Anderson Emily C. O'Brien Emily N. Grayek James K. Hermansen Heather K. Hunt |
| author_facet | Mark E. Anderson Emily C. O'Brien Emily N. Grayek James K. Hermansen Heather K. Hunt |
| author_sort | Mark E. Anderson |
| collection | DOAJ |
| description | Current bacterial detection techniques are relatively slow, require bulky instrumentation, and usually require some form of specialized training. The gold standard for bacterial detection is culture testing, which can take several days to receive a viable result. Therefore, simpler detection techniques that are both fast and sensitive could greatly improve bacterial detection and identification. Here, we present a new method for the detection of the bacteria Helicobacter hepaticus using whispering-gallery mode (WGM) optical microcavity-based sensors. Due to minimal reflection losses and low material adsorption, WGM-based sensors have ultra-high quality factors, resulting in high-sensitivity sensor devices. In this study, we have shown that bacteria can be non-specifically detected using WGM optical microcavity-based sensors. The minimum detection for the device was 1 × 104 cells/mL, and the minimum time of detection was found to be 750 s. Given that a cell density as low as 1 × 103 cells/mL for Helicobacter hepaticus can cause infection, the limit of detection shown here would be useful for most levels where Helicobacter hepaticus is biologically relevant. This study suggests a new approach for H. hepaticus detection using label-free optical sensors that is faster than, and potentially as sensitive as, standard techniques. |
| first_indexed | 2024-12-17T07:18:23Z |
| format | Article |
| id | doaj.art-596bce7bdb1d44f0936fa85c5d495eab |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-12-17T07:18:23Z |
| publishDate | 2015-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-596bce7bdb1d44f0936fa85c5d495eab2022-12-21T21:58:50ZengMDPI AGBiosensors2079-63742015-08-015356257610.3390/bios5030562bios5030562The Detection of Helicobacter hepaticus Using Whispering-Gallery Mode Microcavity Optical SensorsMark E. Anderson0Emily C. O'Brien1Emily N. Grayek2James K. Hermansen3Heather K. Hunt4Department of Biochemistry, University of Missouri, Columbia, MO 65211, USADepartment of Bioengineering, University of Missouri, Columbia, MO 65211, USADepartment of Bioengineering, University of Missouri, Columbia, MO 65211, USADepartment of Bioengineering, University of Missouri, Columbia, MO 65211, USADepartment of Bioengineering, University of Missouri, Columbia, MO 65211, USACurrent bacterial detection techniques are relatively slow, require bulky instrumentation, and usually require some form of specialized training. The gold standard for bacterial detection is culture testing, which can take several days to receive a viable result. Therefore, simpler detection techniques that are both fast and sensitive could greatly improve bacterial detection and identification. Here, we present a new method for the detection of the bacteria Helicobacter hepaticus using whispering-gallery mode (WGM) optical microcavity-based sensors. Due to minimal reflection losses and low material adsorption, WGM-based sensors have ultra-high quality factors, resulting in high-sensitivity sensor devices. In this study, we have shown that bacteria can be non-specifically detected using WGM optical microcavity-based sensors. The minimum detection for the device was 1 × 104 cells/mL, and the minimum time of detection was found to be 750 s. Given that a cell density as low as 1 × 103 cells/mL for Helicobacter hepaticus can cause infection, the limit of detection shown here would be useful for most levels where Helicobacter hepaticus is biologically relevant. This study suggests a new approach for H. hepaticus detection using label-free optical sensors that is faster than, and potentially as sensitive as, standard techniques.http://www.mdpi.com/2079-6374/5/3/562H. hepaticus sensorsmicrocavitiesbacterial detectionoptical transducing mechanisms |
| spellingShingle | Mark E. Anderson Emily C. O'Brien Emily N. Grayek James K. Hermansen Heather K. Hunt The Detection of Helicobacter hepaticus Using Whispering-Gallery Mode Microcavity Optical Sensors Biosensors H. hepaticus sensors microcavities bacterial detection optical transducing mechanisms |
| title | The Detection of Helicobacter hepaticus Using Whispering-Gallery Mode Microcavity Optical Sensors |
| title_full | The Detection of Helicobacter hepaticus Using Whispering-Gallery Mode Microcavity Optical Sensors |
| title_fullStr | The Detection of Helicobacter hepaticus Using Whispering-Gallery Mode Microcavity Optical Sensors |
| title_full_unstemmed | The Detection of Helicobacter hepaticus Using Whispering-Gallery Mode Microcavity Optical Sensors |
| title_short | The Detection of Helicobacter hepaticus Using Whispering-Gallery Mode Microcavity Optical Sensors |
| title_sort | detection of helicobacter hepaticus using whispering gallery mode microcavity optical sensors |
| topic | H. hepaticus sensors microcavities bacterial detection optical transducing mechanisms |
| url | http://www.mdpi.com/2079-6374/5/3/562 |
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