Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure
Monitoring the spore life cycle is one of the main issues in several fields including environmental control, sustainable ecosystems, food security, and healthcare systems. In this framework, the study of the living organism resistance to extreme conditions like those mimicking space environments is...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-07-01
|
| Series: | Sensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8220/20/15/4150 |
| _version_ | 1797561301203943424 |
|---|---|
| author | Carlo Camerlingo Giuseppe Di Meo Maria Lepore Mikhail Lisitskiy Annarita Poli Marianna Portaccio Ida Romano Paola Di Donato |
| author_facet | Carlo Camerlingo Giuseppe Di Meo Maria Lepore Mikhail Lisitskiy Annarita Poli Marianna Portaccio Ida Romano Paola Di Donato |
| author_sort | Carlo Camerlingo |
| collection | DOAJ |
| description | Monitoring the spore life cycle is one of the main issues in several fields including environmental control, sustainable ecosystems, food security, and healthcare systems. In this framework, the study of the living organism resistance to extreme conditions like those mimicking space environments is particularly interesting. The assessment of the local change of the pH level can be extremely useful for this purpose. An optical physiometer method based on the Raman response of the graphene, which is able to locally sense pH of a fluid on a micrometric scale, has been recently proposed. Due to the presence of <inline-formula> <math display="inline"> <semantics> <mi>π</mi> </semantics> </math> </inline-formula>-bonds at the surface, the electronic doping of graphene is determined by the external conditions and can be electrochemically controlled or altered by the contact with an acid or alkaline fluid. The doping level affects the vibrational energies of the graphene that can be monitored by conventional Raman spectroscopy. In addition, Surface-Enhanced Raman Spectroscopy (SERS) can give direct information on the biochemical changes occurring in spore components. In this work, we propose the joint use of Graphene-Based Raman Spectroscopy (GbRS) and SERS for the monitoring of the response of spores to exposure to low temperatures down to 100 K. The spores of the thermophilic bacterium <i>Parageobacillus thermantarcticus</i> isolated from an active volcano of Antarctica (Mt. Melbourne) were investigated. These spores are particularly resistant to several stressing stimuli and able to adapt to extreme conditions like low temperatures, UV irradiation, and <inline-formula> <math display="inline"> <semantics> <mi>γ</mi> </semantics> </math> </inline-formula>-rays exposure. The results obtained showed that the joint use of GbRS and SERS represents a valuable tool for monitoring the physio-chemical response of bacterial spores upon exposure to stressing stimuli. |
| first_indexed | 2024-03-10T18:11:59Z |
| format | Article |
| id | doaj.art-596c84ff1d894e7195eac7f2a0224132 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T18:11:59Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-596c84ff1d894e7195eac7f2a02241322023-11-20T07:59:26ZengMDPI AGSensors1424-82202020-07-012015415010.3390/s20154150Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures ExposureCarlo Camerlingo0Giuseppe Di Meo1Maria Lepore2Mikhail Lisitskiy3Annarita Poli4Marianna Portaccio5Ida Romano6Paola Di Donato7CNR-SPIN, Institute for Superconductivity, Innovative Materials and Devices, 80078 Pozzuoli, ItalyCNR-ICB, Institute of Biomolecular Chemistry, 80078 Pozzuoli, ItalyDipartimento di Medicina Sperimentale, Università della Campania “L. Vanvitelli”, Via S. Maria di Costantinopoli 16, 80138 Napoli, ItalyCNR-SPIN, Institute for Superconductivity, Innovative Materials and Devices, 80078 Pozzuoli, ItalyCNR-ICB, Institute of Biomolecular Chemistry, 80078 Pozzuoli, ItalyDipartimento di Medicina Sperimentale, Università della Campania “L. Vanvitelli”, Via S. Maria di Costantinopoli 16, 80138 Napoli, ItalyCNR-ICB, Institute of Biomolecular Chemistry, 80078 Pozzuoli, ItalyCNR-ICB, Institute of Biomolecular Chemistry, 80078 Pozzuoli, ItalyMonitoring the spore life cycle is one of the main issues in several fields including environmental control, sustainable ecosystems, food security, and healthcare systems. In this framework, the study of the living organism resistance to extreme conditions like those mimicking space environments is particularly interesting. The assessment of the local change of the pH level can be extremely useful for this purpose. An optical physiometer method based on the Raman response of the graphene, which is able to locally sense pH of a fluid on a micrometric scale, has been recently proposed. Due to the presence of <inline-formula> <math display="inline"> <semantics> <mi>π</mi> </semantics> </math> </inline-formula>-bonds at the surface, the electronic doping of graphene is determined by the external conditions and can be electrochemically controlled or altered by the contact with an acid or alkaline fluid. The doping level affects the vibrational energies of the graphene that can be monitored by conventional Raman spectroscopy. In addition, Surface-Enhanced Raman Spectroscopy (SERS) can give direct information on the biochemical changes occurring in spore components. In this work, we propose the joint use of Graphene-Based Raman Spectroscopy (GbRS) and SERS for the monitoring of the response of spores to exposure to low temperatures down to 100 K. The spores of the thermophilic bacterium <i>Parageobacillus thermantarcticus</i> isolated from an active volcano of Antarctica (Mt. Melbourne) were investigated. These spores are particularly resistant to several stressing stimuli and able to adapt to extreme conditions like low temperatures, UV irradiation, and <inline-formula> <math display="inline"> <semantics> <mi>γ</mi> </semantics> </math> </inline-formula>-rays exposure. The results obtained showed that the joint use of GbRS and SERS represents a valuable tool for monitoring the physio-chemical response of bacterial spores upon exposure to stressing stimuli.https://www.mdpi.com/1424-8220/20/15/4150thermophilic bacteriaspore germinationSERSgraphene-based pH-meter |
| spellingShingle | Carlo Camerlingo Giuseppe Di Meo Maria Lepore Mikhail Lisitskiy Annarita Poli Marianna Portaccio Ida Romano Paola Di Donato Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure Sensors thermophilic bacteria spore germination SERS graphene-based pH-meter |
| title | Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure |
| title_full | Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure |
| title_fullStr | Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure |
| title_full_unstemmed | Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure |
| title_short | Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure |
| title_sort | graphene based and surface enhanced raman spectroscopy for monitoring the physio chemical response of thermophilic bacterial spores to low temperatures exposure |
| topic | thermophilic bacteria spore germination SERS graphene-based pH-meter |
| url | https://www.mdpi.com/1424-8220/20/15/4150 |
| work_keys_str_mv | AT carlocamerlingo graphenebasedandsurfaceenhancedramanspectroscopyformonitoringthephysiochemicalresponseofthermophilicbacterialsporestolowtemperaturesexposure AT giuseppedimeo graphenebasedandsurfaceenhancedramanspectroscopyformonitoringthephysiochemicalresponseofthermophilicbacterialsporestolowtemperaturesexposure AT marialepore graphenebasedandsurfaceenhancedramanspectroscopyformonitoringthephysiochemicalresponseofthermophilicbacterialsporestolowtemperaturesexposure AT mikhaillisitskiy graphenebasedandsurfaceenhancedramanspectroscopyformonitoringthephysiochemicalresponseofthermophilicbacterialsporestolowtemperaturesexposure AT annaritapoli graphenebasedandsurfaceenhancedramanspectroscopyformonitoringthephysiochemicalresponseofthermophilicbacterialsporestolowtemperaturesexposure AT mariannaportaccio graphenebasedandsurfaceenhancedramanspectroscopyformonitoringthephysiochemicalresponseofthermophilicbacterialsporestolowtemperaturesexposure AT idaromano graphenebasedandsurfaceenhancedramanspectroscopyformonitoringthephysiochemicalresponseofthermophilicbacterialsporestolowtemperaturesexposure AT paoladidonato graphenebasedandsurfaceenhancedramanspectroscopyformonitoringthephysiochemicalresponseofthermophilicbacterialsporestolowtemperaturesexposure |