Elicitation of salt stress-tolerant mutants in bread wheat (Triticum aestivum L.) by using gamma radiation
Abstract Background Given the strategic importance of wheat being the staple food for the vast majority of people, it was necessary to know reasons for the contraction and decline of its area globally and consequently its lower yield. Among the most important of these problems is the problem of a hi...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-07-01
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| Series: | Bulletin of the National Research Centre |
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| Online Access: | http://link.springer.com/article/10.1186/s42269-020-00357-1 |
| _version_ | 1819071822504656896 |
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| author | Almoataz Bellah Ali El-Mouhamady Hayam Fouad Ibrahim |
| author_facet | Almoataz Bellah Ali El-Mouhamady Hayam Fouad Ibrahim |
| author_sort | Almoataz Bellah Ali El-Mouhamady |
| collection | DOAJ |
| description | Abstract Background Given the strategic importance of wheat being the staple food for the vast majority of people, it was necessary to know reasons for the contraction and decline of its area globally and consequently its lower yield. Among the most important of these problems is the problem of a high level of salinity in both soil and irrigation water coming at the forefront of environmental challenges that hinder its production process at the local and global levels. Therefore, the genetic improvement of wheat for salinity tolerance was one of the most important priorities of this investigation. Results The seven wheat accessions (Sakha 8 and its six M5 derived mutants) succeeded in drawing unique cases of salinity tolerance and were excellent especially mutants 1, 2, 3, and 5. The rest genotypes were coming in the second rank for this purpose and were very good in this regard. The promising wheat genotypes which recorded high salinity tolerance in the recent investigation exhibited also high genetic stability. This fact was proved after estimating some agro-morphological and physiological traits related to salinity tolerance based on evaluating some important genetic parameters besides salinity tolerance indices under stress experiment compared to the control treatment within two seasons. Data evaluated of expected genetic advance (GA) based on 5% selection proved that all values calculated during the two seasons under both treatments appeared low for all studied traits in this regard. However, it reflects the success of breeding for salinity tolerance in wheat using mutations but in relative terms. Molecular marker analysis profile using the six ISSR primers exhibited a total of 173 markers, 12 of them were monomorphic, while that 161 bands appeared polymorphic included 56 unique bands or positive markers and 7 negative markers with 93.06 % (polymorphism). Conclusion The original wheat variety (Sakha 8) and its six M5 derived mutants exhibited high tolerance of salinity stress in all studied traits based on all genetic parameters and salinity tolerance indices calculated for both seasons under salinity treatment compared to the normal conditions. DNA fingerprinting analysis as well for the six wheat mutants besides the local variety proved that these genotypes were recorded highly genetic differences among them. |
| first_indexed | 2024-12-21T17:27:56Z |
| format | Article |
| id | doaj.art-596cb87b07c7445eb696bc4b0972ef17 |
| institution | Directory Open Access Journal |
| issn | 2522-8307 |
| language | English |
| last_indexed | 2024-12-21T17:27:56Z |
| publishDate | 2020-07-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bulletin of the National Research Centre |
| spelling | doaj.art-596cb87b07c7445eb696bc4b0972ef172022-12-21T18:56:00ZengSpringerOpenBulletin of the National Research Centre2522-83072020-07-0144111810.1186/s42269-020-00357-1Elicitation of salt stress-tolerant mutants in bread wheat (Triticum aestivum L.) by using gamma radiationAlmoataz Bellah Ali El-Mouhamady0Hayam Fouad Ibrahim1Genetics and Cytology Department, Genetic Engineering and Biotechnology Research Division, National Research CentreGenetics and Cytology Department, Genetic Engineering and Biotechnology Research Division, National Research CentreAbstract Background Given the strategic importance of wheat being the staple food for the vast majority of people, it was necessary to know reasons for the contraction and decline of its area globally and consequently its lower yield. Among the most important of these problems is the problem of a high level of salinity in both soil and irrigation water coming at the forefront of environmental challenges that hinder its production process at the local and global levels. Therefore, the genetic improvement of wheat for salinity tolerance was one of the most important priorities of this investigation. Results The seven wheat accessions (Sakha 8 and its six M5 derived mutants) succeeded in drawing unique cases of salinity tolerance and were excellent especially mutants 1, 2, 3, and 5. The rest genotypes were coming in the second rank for this purpose and were very good in this regard. The promising wheat genotypes which recorded high salinity tolerance in the recent investigation exhibited also high genetic stability. This fact was proved after estimating some agro-morphological and physiological traits related to salinity tolerance based on evaluating some important genetic parameters besides salinity tolerance indices under stress experiment compared to the control treatment within two seasons. Data evaluated of expected genetic advance (GA) based on 5% selection proved that all values calculated during the two seasons under both treatments appeared low for all studied traits in this regard. However, it reflects the success of breeding for salinity tolerance in wheat using mutations but in relative terms. Molecular marker analysis profile using the six ISSR primers exhibited a total of 173 markers, 12 of them were monomorphic, while that 161 bands appeared polymorphic included 56 unique bands or positive markers and 7 negative markers with 93.06 % (polymorphism). Conclusion The original wheat variety (Sakha 8) and its six M5 derived mutants exhibited high tolerance of salinity stress in all studied traits based on all genetic parameters and salinity tolerance indices calculated for both seasons under salinity treatment compared to the normal conditions. DNA fingerprinting analysis as well for the six wheat mutants besides the local variety proved that these genotypes were recorded highly genetic differences among them.http://link.springer.com/article/10.1186/s42269-020-00357-1WheatSalinity toleranceGamma raysMutationGenetic variationHeritability |
| spellingShingle | Almoataz Bellah Ali El-Mouhamady Hayam Fouad Ibrahim Elicitation of salt stress-tolerant mutants in bread wheat (Triticum aestivum L.) by using gamma radiation Bulletin of the National Research Centre Wheat Salinity tolerance Gamma rays Mutation Genetic variation Heritability |
| title | Elicitation of salt stress-tolerant mutants in bread wheat (Triticum aestivum L.) by using gamma radiation |
| title_full | Elicitation of salt stress-tolerant mutants in bread wheat (Triticum aestivum L.) by using gamma radiation |
| title_fullStr | Elicitation of salt stress-tolerant mutants in bread wheat (Triticum aestivum L.) by using gamma radiation |
| title_full_unstemmed | Elicitation of salt stress-tolerant mutants in bread wheat (Triticum aestivum L.) by using gamma radiation |
| title_short | Elicitation of salt stress-tolerant mutants in bread wheat (Triticum aestivum L.) by using gamma radiation |
| title_sort | elicitation of salt stress tolerant mutants in bread wheat triticum aestivum l by using gamma radiation |
| topic | Wheat Salinity tolerance Gamma rays Mutation Genetic variation Heritability |
| url | http://link.springer.com/article/10.1186/s42269-020-00357-1 |
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