Molecular Discrimination for Two Anadenanthera Species of Seasonally Dry Tropical Forest Remnants in Brazil
<i>Anadenanthera colubrina</i> (Acol) and <i>Anadenanthera peregrina</i> (Aper) (Fabaceae) are two species popularly known as “angicos” that occur in seasonally dry tropical forest (SDTR) remnants in Brazil. Since many of the morphological characteristics of <i>Anadenan...
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MDPI AG
2024-02-01
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| Series: | Taxonomy |
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| Online Access: | https://www.mdpi.com/2673-6500/4/1/8 |
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| author | Fernando Bonifácio-Anacleto Rômulo Maciel Moraes Filho Leonardo Maurici Borges Carlos Alberto Martinez Ana Lilia Alzate-Marin |
| author_facet | Fernando Bonifácio-Anacleto Rômulo Maciel Moraes Filho Leonardo Maurici Borges Carlos Alberto Martinez Ana Lilia Alzate-Marin |
| author_sort | Fernando Bonifácio-Anacleto |
| collection | DOAJ |
| description | <i>Anadenanthera colubrina</i> (Acol) and <i>Anadenanthera peregrina</i> (Aper) (Fabaceae) are two species popularly known as “angicos” that occur in seasonally dry tropical forest (SDTR) remnants in Brazil. Since many of the morphological characteristics of <i>Anadenanthera</i> species are superimposed and species-specific characteristics are difficult to observe, their identification is complex. Therefore, in this research, a set of ISSR (Inter-Simple Sequence Repeat Polymorphic DNA) molecular markers was standardized, aiming to characterize <i>A. colubrina</i> and <i>A. peregrina</i> species and study the genetic diversity of three populations of each species located within a fragmented landscape in São Paulo State, southeastern Brazil. Seven ISSR markers (UBC 2, 820, 851, 858, 864, 866, and 886) that show polymorphism for both species were used. The Bayesian cluster, PCoA and dendrogram analysis show that the total sample divides into two groups corresponding to each species. Also, a genetic divergence (Gst = 0.143) and a high number of migrants per generation (Nm = 3.0) were detected between them. The Acol populations showed significantly higher values for mean genetic diversity (<i>h</i> = 0.30) than Aper (<i>h</i> = 0.25) (<i>p</i> < 0.05). The ISSR marker UBC2<sub>250bp</sub> showed species-specific electrophoretic fingerprints for both species. The molecular tools generated herein support the conservation of <i>Anadenanthera</i> sp. and the restoration of vegetation where the species naturally occurs. |
| first_indexed | 2024-04-24T17:48:17Z |
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| issn | 2673-6500 |
| language | English |
| last_indexed | 2024-04-24T17:48:17Z |
| publishDate | 2024-02-01 |
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| record_format | Article |
| series | Taxonomy |
| spelling | doaj.art-596d8697e7454bc491029edd2dc7fe752024-03-27T14:05:48ZengMDPI AGTaxonomy2673-65002024-02-014115016210.3390/taxonomy4010008Molecular Discrimination for Two Anadenanthera Species of Seasonally Dry Tropical Forest Remnants in BrazilFernando Bonifácio-Anacleto0Rômulo Maciel Moraes Filho1Leonardo Maurici Borges2Carlos Alberto Martinez3Ana Lilia Alzate-Marin4Laboratório de Genética Vegetal, Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes 3900, Ribeirão Preto 14049-900, SP, BrazilLaboratório de Genética Vegetal, Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes 3900, Ribeirão Preto 14049-900, SP, BrazilDepartamento de Botânica, Universidade Federal de São Carlos, Rodovia Washington Luís, Km 235, São Carlos 13565-905, SP, BrazilDepartamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes 3900, Ribeirão Preto 14040-030, SP, BrazilLaboratório de Genética Vegetal, Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes 3900, Ribeirão Preto 14049-900, SP, Brazil<i>Anadenanthera colubrina</i> (Acol) and <i>Anadenanthera peregrina</i> (Aper) (Fabaceae) are two species popularly known as “angicos” that occur in seasonally dry tropical forest (SDTR) remnants in Brazil. Since many of the morphological characteristics of <i>Anadenanthera</i> species are superimposed and species-specific characteristics are difficult to observe, their identification is complex. Therefore, in this research, a set of ISSR (Inter-Simple Sequence Repeat Polymorphic DNA) molecular markers was standardized, aiming to characterize <i>A. colubrina</i> and <i>A. peregrina</i> species and study the genetic diversity of three populations of each species located within a fragmented landscape in São Paulo State, southeastern Brazil. Seven ISSR markers (UBC 2, 820, 851, 858, 864, 866, and 886) that show polymorphism for both species were used. The Bayesian cluster, PCoA and dendrogram analysis show that the total sample divides into two groups corresponding to each species. Also, a genetic divergence (Gst = 0.143) and a high number of migrants per generation (Nm = 3.0) were detected between them. The Acol populations showed significantly higher values for mean genetic diversity (<i>h</i> = 0.30) than Aper (<i>h</i> = 0.25) (<i>p</i> < 0.05). The ISSR marker UBC2<sub>250bp</sub> showed species-specific electrophoretic fingerprints for both species. The molecular tools generated herein support the conservation of <i>Anadenanthera</i> sp. and the restoration of vegetation where the species naturally occurs.https://www.mdpi.com/2673-6500/4/1/8<i>Anadenanthera</i>forest conservationgenetic diversitymolecular markersseasonally dry tropical forests—SDTF |
| spellingShingle | Fernando Bonifácio-Anacleto Rômulo Maciel Moraes Filho Leonardo Maurici Borges Carlos Alberto Martinez Ana Lilia Alzate-Marin Molecular Discrimination for Two Anadenanthera Species of Seasonally Dry Tropical Forest Remnants in Brazil Taxonomy <i>Anadenanthera</i> forest conservation genetic diversity molecular markers seasonally dry tropical forests—SDTF |
| title | Molecular Discrimination for Two Anadenanthera Species of Seasonally Dry Tropical Forest Remnants in Brazil |
| title_full | Molecular Discrimination for Two Anadenanthera Species of Seasonally Dry Tropical Forest Remnants in Brazil |
| title_fullStr | Molecular Discrimination for Two Anadenanthera Species of Seasonally Dry Tropical Forest Remnants in Brazil |
| title_full_unstemmed | Molecular Discrimination for Two Anadenanthera Species of Seasonally Dry Tropical Forest Remnants in Brazil |
| title_short | Molecular Discrimination for Two Anadenanthera Species of Seasonally Dry Tropical Forest Remnants in Brazil |
| title_sort | molecular discrimination for two anadenanthera species of seasonally dry tropical forest remnants in brazil |
| topic | <i>Anadenanthera</i> forest conservation genetic diversity molecular markers seasonally dry tropical forests—SDTF |
| url | https://www.mdpi.com/2673-6500/4/1/8 |
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