Detection of Glutamate Alterations in the Human Brain Using 1H-MRS: Comparison of STEAM and sLASER at 7 T
PurposeTo assess reproducibility of glutamate measurement in the human brain by two short echo time (TE) 1H-MRS sequences [stimulated echo acquisition mode (STEAM) and semi-localized by adiabatic selective refocusing (sLASER)] at 7 T. Reliable assessment of glutamate is important when studying a var...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-04-01
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| Series: | Frontiers in Psychiatry |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpsyt.2017.00060/full |
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| author | René C. W. Mandl Anouk Marsman Vincent O. Boer Peter R. Luijten Hilleke E. Hulshoff Pol Dennis W. J. Klomp |
| author_facet | René C. W. Mandl Anouk Marsman Vincent O. Boer Peter R. Luijten Hilleke E. Hulshoff Pol Dennis W. J. Klomp |
| author_sort | René C. W. Mandl |
| collection | DOAJ |
| description | PurposeTo assess reproducibility of glutamate measurement in the human brain by two short echo time (TE) 1H-MRS sequences [stimulated echo acquisition mode (STEAM) and semi-localized by adiabatic selective refocusing (sLASER)] at 7 T. Reliable assessment of glutamate is important when studying a variety of neurological and neuropsychiatric disorders. At 7 T, the glutamate signal can be separated from the glutamine signal and hence more accurately measured as compared to lower field strengths. A sLASER sequence has been developed for 7 T, using field focusing at short TE, resulting in twice as much signal as can be obtained using STEAM and improved localization accuracy due to a decreased chemical shift artifact.Materials and methodsEight subjects were scanned twice using both STEAM and sLASER. Data were acquired from the frontal and occipital brain region. Subsequently, intraclass correlations were computed for the estimated metabolite concentrations.ResultssLASER has higher ICC’s for glutamate concentration as compared to STEAM in both the frontal and occipital VOI, which is probably due to the higher sensitivity and localization accuracy.ConclusionWe conclude that sLASER 1H-MRS at 7 T is a reliable method to obtain reproducible measures of glutamate levels in the human brain at such high accuracy that individual variability, even between age-matched subjects, is measured. |
| first_indexed | 2024-12-11T11:03:38Z |
| format | Article |
| id | doaj.art-596f34e852804d3fb50b60dafa37688f |
| institution | Directory Open Access Journal |
| issn | 1664-0640 |
| language | English |
| last_indexed | 2024-12-11T11:03:38Z |
| publishDate | 2017-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Psychiatry |
| spelling | doaj.art-596f34e852804d3fb50b60dafa37688f2022-12-22T01:09:47ZengFrontiers Media S.A.Frontiers in Psychiatry1664-06402017-04-01810.3389/fpsyt.2017.00060263901Detection of Glutamate Alterations in the Human Brain Using 1H-MRS: Comparison of STEAM and sLASER at 7 TRené C. W. Mandl0Anouk Marsman1Vincent O. Boer2Peter R. Luijten3Hilleke E. Hulshoff Pol4Dennis W. J. Klomp5Psychiatry, Brain Center Rudolf Magnus, University Medical Center Utrecht, Utrecht, NetherlandsPsychiatry, Brain Center Rudolf Magnus, University Medical Center Utrecht, Utrecht, NetherlandsRadiology, Brain Center Rudolf Magnus, University Medical Center Utrecht, Utrecht, NetherlandsRadiology, Brain Center Rudolf Magnus, University Medical Center Utrecht, Utrecht, NetherlandsPsychiatry, Brain Center Rudolf Magnus, University Medical Center Utrecht, Utrecht, NetherlandsRadiology, Brain Center Rudolf Magnus, University Medical Center Utrecht, Utrecht, NetherlandsPurposeTo assess reproducibility of glutamate measurement in the human brain by two short echo time (TE) 1H-MRS sequences [stimulated echo acquisition mode (STEAM) and semi-localized by adiabatic selective refocusing (sLASER)] at 7 T. Reliable assessment of glutamate is important when studying a variety of neurological and neuropsychiatric disorders. At 7 T, the glutamate signal can be separated from the glutamine signal and hence more accurately measured as compared to lower field strengths. A sLASER sequence has been developed for 7 T, using field focusing at short TE, resulting in twice as much signal as can be obtained using STEAM and improved localization accuracy due to a decreased chemical shift artifact.Materials and methodsEight subjects were scanned twice using both STEAM and sLASER. Data were acquired from the frontal and occipital brain region. Subsequently, intraclass correlations were computed for the estimated metabolite concentrations.ResultssLASER has higher ICC’s for glutamate concentration as compared to STEAM in both the frontal and occipital VOI, which is probably due to the higher sensitivity and localization accuracy.ConclusionWe conclude that sLASER 1H-MRS at 7 T is a reliable method to obtain reproducible measures of glutamate levels in the human brain at such high accuracy that individual variability, even between age-matched subjects, is measured.http://journal.frontiersin.org/article/10.3389/fpsyt.2017.00060/fullglutamatemagnetic resonance spectroscopyreproducibilitybrain7 T |
| spellingShingle | René C. W. Mandl Anouk Marsman Vincent O. Boer Peter R. Luijten Hilleke E. Hulshoff Pol Dennis W. J. Klomp Detection of Glutamate Alterations in the Human Brain Using 1H-MRS: Comparison of STEAM and sLASER at 7 T Frontiers in Psychiatry glutamate magnetic resonance spectroscopy reproducibility brain 7 T |
| title | Detection of Glutamate Alterations in the Human Brain Using 1H-MRS: Comparison of STEAM and sLASER at 7 T |
| title_full | Detection of Glutamate Alterations in the Human Brain Using 1H-MRS: Comparison of STEAM and sLASER at 7 T |
| title_fullStr | Detection of Glutamate Alterations in the Human Brain Using 1H-MRS: Comparison of STEAM and sLASER at 7 T |
| title_full_unstemmed | Detection of Glutamate Alterations in the Human Brain Using 1H-MRS: Comparison of STEAM and sLASER at 7 T |
| title_short | Detection of Glutamate Alterations in the Human Brain Using 1H-MRS: Comparison of STEAM and sLASER at 7 T |
| title_sort | detection of glutamate alterations in the human brain using 1h mrs comparison of steam and slaser at 7 t |
| topic | glutamate magnetic resonance spectroscopy reproducibility brain 7 T |
| url | http://journal.frontiersin.org/article/10.3389/fpsyt.2017.00060/full |
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