Cell type-specific Effect of miRZip-21 to Suppress miR-21 in Human Glioma Cell Lines
There are different subtypes of brain tumors, classified according to the origin of the abnormally proliferated glial cells. Glioblastoma multiforma (GBM) is the grade 4 of brain tumors, gliomas, with the least life expectancy. microRNAs (miRNAs) are small, single stranded, non-coding RNAs with 20-2...
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| Format: | Article |
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Ferdowsi University of Mashhad
2019-03-01
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| Series: | Journal of Cell and Molecular Research |
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| Online Access: | https://jcmr.um.ac.ir/article_29485_eb65dd30d8dad1327b97d9f8277f2702.pdf |
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| author | Hamideh Monfared Yavar Jahangard Maryam Nikkhah Seyed Javad Mirnajafi-Zade Seyed Javad Mowla |
| author_facet | Hamideh Monfared Yavar Jahangard Maryam Nikkhah Seyed Javad Mirnajafi-Zade Seyed Javad Mowla |
| author_sort | Hamideh Monfared |
| collection | DOAJ |
| description | There are different subtypes of brain tumors, classified according to the origin of the abnormally proliferated glial cells. Glioblastoma multiforma (GBM) is the grade 4 of brain tumors, gliomas, with the least life expectancy. microRNAs (miRNAs) are small, single stranded, non-coding RNAs with 20-25 nt length with post-transcriptional gene regulatory activities. An altered expression of miRNAs is linked to developmental disorders and some diseases, most importantly cancers. miR-21 is a well-known microRNA, overexpressed in almost all cancer types, including brain tumors. It targets several genes with vital roles in cellular pathways involve in proliferation, invasion and metastatic behaviors. Exosomes are 30-100 nm extracellular vesicles which are packed with various molecules, including miRNAs. Here, we suppressed miR-21 expression level in HEK-293T cells by transfecting them with the miRZip-21 vector. However, when U87-MG cells were cultured in the presence of exosomes isolated from conditioned medium of engineered HEK-293T cells derived exosomes, we did not observe any suppressing effect on host cells’ miR-21 expression level. Moreover, by analyzing the effects of miRZip-21-enriched cell’s conditioned media on three other brain cell lines including 1321N1, A-172 and DAOY, cell type-specific effects of exocrine miRZip-21 were revealed. These data suggested that cell lines from different brain tumor subtypes could exert different responses to microRNA-based therapies, based on their cellular origin and clinical behaviors. |
| first_indexed | 2024-12-13T13:36:32Z |
| format | Article |
| id | doaj.art-59704028c4964f9fbd934c21e21f2523 |
| institution | Directory Open Access Journal |
| issn | 2008-9147 2717-3364 |
| language | English |
| last_indexed | 2024-12-13T13:36:32Z |
| publishDate | 2019-03-01 |
| publisher | Ferdowsi University of Mashhad |
| record_format | Article |
| series | Journal of Cell and Molecular Research |
| spelling | doaj.art-59704028c4964f9fbd934c21e21f25232022-12-21T23:43:47ZengFerdowsi University of MashhadJournal of Cell and Molecular Research2008-91472717-33642019-03-01102596610.22067/jcmr.v10i2.7683529485Cell type-specific Effect of miRZip-21 to Suppress miR-21 in Human Glioma Cell LinesHamideh Monfared0Yavar Jahangard1Maryam Nikkhah2Seyed Javad Mirnajafi-Zade3Seyed Javad Mowla4Tarbiat Modares University, Tehran, IranTarbiat Modares University, Tehran, IranTarbiat Modares University, Tehran, IranTarbiat Modares University, Tehran, IranTarbiat Modares University, Tehran, IranThere are different subtypes of brain tumors, classified according to the origin of the abnormally proliferated glial cells. Glioblastoma multiforma (GBM) is the grade 4 of brain tumors, gliomas, with the least life expectancy. microRNAs (miRNAs) are small, single stranded, non-coding RNAs with 20-25 nt length with post-transcriptional gene regulatory activities. An altered expression of miRNAs is linked to developmental disorders and some diseases, most importantly cancers. miR-21 is a well-known microRNA, overexpressed in almost all cancer types, including brain tumors. It targets several genes with vital roles in cellular pathways involve in proliferation, invasion and metastatic behaviors. Exosomes are 30-100 nm extracellular vesicles which are packed with various molecules, including miRNAs. Here, we suppressed miR-21 expression level in HEK-293T cells by transfecting them with the miRZip-21 vector. However, when U87-MG cells were cultured in the presence of exosomes isolated from conditioned medium of engineered HEK-293T cells derived exosomes, we did not observe any suppressing effect on host cells’ miR-21 expression level. Moreover, by analyzing the effects of miRZip-21-enriched cell’s conditioned media on three other brain cell lines including 1321N1, A-172 and DAOY, cell type-specific effects of exocrine miRZip-21 were revealed. These data suggested that cell lines from different brain tumor subtypes could exert different responses to microRNA-based therapies, based on their cellular origin and clinical behaviors.https://jcmr.um.ac.ir/article_29485_eb65dd30d8dad1327b97d9f8277f2702.pdfmir-21brain tumorsglioblastoma multiformaexosomes |
| spellingShingle | Hamideh Monfared Yavar Jahangard Maryam Nikkhah Seyed Javad Mirnajafi-Zade Seyed Javad Mowla Cell type-specific Effect of miRZip-21 to Suppress miR-21 in Human Glioma Cell Lines Journal of Cell and Molecular Research mir-21 brain tumors glioblastoma multiforma exosomes |
| title | Cell type-specific Effect of miRZip-21 to Suppress miR-21 in Human Glioma Cell Lines |
| title_full | Cell type-specific Effect of miRZip-21 to Suppress miR-21 in Human Glioma Cell Lines |
| title_fullStr | Cell type-specific Effect of miRZip-21 to Suppress miR-21 in Human Glioma Cell Lines |
| title_full_unstemmed | Cell type-specific Effect of miRZip-21 to Suppress miR-21 in Human Glioma Cell Lines |
| title_short | Cell type-specific Effect of miRZip-21 to Suppress miR-21 in Human Glioma Cell Lines |
| title_sort | cell type specific effect of mirzip 21 to suppress mir 21 in human glioma cell lines |
| topic | mir-21 brain tumors glioblastoma multiforma exosomes |
| url | https://jcmr.um.ac.ir/article_29485_eb65dd30d8dad1327b97d9f8277f2702.pdf |
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