Effects of Hypoxia on Proliferation and Differentiation in Belgian Blue and Hanwoo Muscle Satellite Cells for the Development of Cultured Meat
Among future food problems, the demand for meat is expected to increase rapidly, but the production efficiency of meat, which is a protein source, is very low compared to other foods. To address this problem, research on the development and production of cultured meat as an alternative meat source u...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/12/6/838 |
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| author | Sanghun Park Mick Gagliardi Geertje Swennen Arin Dogan Yuna Kim Yunhwan Park Gyutae Park Sehyuk Oh Mark Post Jungseok Choi |
| author_facet | Sanghun Park Mick Gagliardi Geertje Swennen Arin Dogan Yuna Kim Yunhwan Park Gyutae Park Sehyuk Oh Mark Post Jungseok Choi |
| author_sort | Sanghun Park |
| collection | DOAJ |
| description | Among future food problems, the demand for meat is expected to increase rapidly, but the production efficiency of meat, which is a protein source, is very low compared to other foods. To address this problem, research on the development and production of cultured meat as an alternative meat source using muscle stem cells in vitro has recently been undertaken. Many studies have been conducted on myosatellite cells for medical purposes, but studies on alternative meat production are rare. In vitro cell culture mimics the in vivo environment for cell growth. The satellite cell niche is closer to hypoxic (2% O<sub>2</sub>) than normoxic (20% O<sub>2</sub>) conditions. The aim of this study was to investigate the efficient oxygen conditions of myosatellite cell cultures for the production of cultured meat. The bovine satellite cell counts and mRNA (Pax7, Myf5 and HIF1α) levels were higher in hypoxia than normoxia (<i>p</i> < 0.05). Through Hoechst-positive nuclei counts, and expression of Pax7, MyoD and myosin protein by immunofluorescence, it was confirmed that muscle cells performed normal proliferation and differentiation. Myoblast fusion was higher under hypoxic conditions (<i>p</i> < 0.05), and the myotube diameters were also thicker (<i>p</i> < 0.05). In the myotube, the number of cells was high in hypoxia, and the expression of the total protein amounts, differentiation marker mRNA (myogenin, myosin and TOM20), and protein markers (myosin and TOM20) was also high. The study results demonstrated that the proliferation and differentiation of bovine myosatellite cells were promoted more highly under hypoxic conditions than under normoxic conditions. Therefore, hypoxic cultures that promote the proliferation and differentiation of bovine myosatellite cells may be an important factor in the development of cultured meat. |
| first_indexed | 2024-03-10T00:17:52Z |
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| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T00:17:52Z |
| publishDate | 2022-06-01 |
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| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-59707accfb4c4fbf8719189c85553e0b2023-11-23T15:47:50ZengMDPI AGBiomolecules2218-273X2022-06-0112683810.3390/biom12060838Effects of Hypoxia on Proliferation and Differentiation in Belgian Blue and Hanwoo Muscle Satellite Cells for the Development of Cultured MeatSanghun Park0Mick Gagliardi1Geertje Swennen2Arin Dogan3Yuna Kim4Yunhwan Park5Gyutae Park6Sehyuk Oh7Mark Post8Jungseok Choi9Department of Animal Science, Chungbuk National University, Cheongju 28644, KoreaMosa Meat B.V., 6229 PM Maastricht, The NetherlandsMosa Meat B.V., 6229 PM Maastricht, The NetherlandsMosa Meat B.V., 6229 PM Maastricht, The NetherlandsDepartment of Animal Science, Chungbuk National University, Cheongju 28644, KoreaDepartment of Animal Science, Chungbuk National University, Cheongju 28644, KoreaDepartment of Animal Science, Chungbuk National University, Cheongju 28644, KoreaDepartment of Animal Science, Chungbuk National University, Cheongju 28644, KoreaMosa Meat B.V., 6229 PM Maastricht, The NetherlandsDepartment of Animal Science, Chungbuk National University, Cheongju 28644, KoreaAmong future food problems, the demand for meat is expected to increase rapidly, but the production efficiency of meat, which is a protein source, is very low compared to other foods. To address this problem, research on the development and production of cultured meat as an alternative meat source using muscle stem cells in vitro has recently been undertaken. Many studies have been conducted on myosatellite cells for medical purposes, but studies on alternative meat production are rare. In vitro cell culture mimics the in vivo environment for cell growth. The satellite cell niche is closer to hypoxic (2% O<sub>2</sub>) than normoxic (20% O<sub>2</sub>) conditions. The aim of this study was to investigate the efficient oxygen conditions of myosatellite cell cultures for the production of cultured meat. The bovine satellite cell counts and mRNA (Pax7, Myf5 and HIF1α) levels were higher in hypoxia than normoxia (<i>p</i> < 0.05). Through Hoechst-positive nuclei counts, and expression of Pax7, MyoD and myosin protein by immunofluorescence, it was confirmed that muscle cells performed normal proliferation and differentiation. Myoblast fusion was higher under hypoxic conditions (<i>p</i> < 0.05), and the myotube diameters were also thicker (<i>p</i> < 0.05). In the myotube, the number of cells was high in hypoxia, and the expression of the total protein amounts, differentiation marker mRNA (myogenin, myosin and TOM20), and protein markers (myosin and TOM20) was also high. The study results demonstrated that the proliferation and differentiation of bovine myosatellite cells were promoted more highly under hypoxic conditions than under normoxic conditions. Therefore, hypoxic cultures that promote the proliferation and differentiation of bovine myosatellite cells may be an important factor in the development of cultured meat.https://www.mdpi.com/2218-273X/12/6/838hypoxianormoxiabovine satellite cellproliferationdifferentiation |
| spellingShingle | Sanghun Park Mick Gagliardi Geertje Swennen Arin Dogan Yuna Kim Yunhwan Park Gyutae Park Sehyuk Oh Mark Post Jungseok Choi Effects of Hypoxia on Proliferation and Differentiation in Belgian Blue and Hanwoo Muscle Satellite Cells for the Development of Cultured Meat Biomolecules hypoxia normoxia bovine satellite cell proliferation differentiation |
| title | Effects of Hypoxia on Proliferation and Differentiation in Belgian Blue and Hanwoo Muscle Satellite Cells for the Development of Cultured Meat |
| title_full | Effects of Hypoxia on Proliferation and Differentiation in Belgian Blue and Hanwoo Muscle Satellite Cells for the Development of Cultured Meat |
| title_fullStr | Effects of Hypoxia on Proliferation and Differentiation in Belgian Blue and Hanwoo Muscle Satellite Cells for the Development of Cultured Meat |
| title_full_unstemmed | Effects of Hypoxia on Proliferation and Differentiation in Belgian Blue and Hanwoo Muscle Satellite Cells for the Development of Cultured Meat |
| title_short | Effects of Hypoxia on Proliferation and Differentiation in Belgian Blue and Hanwoo Muscle Satellite Cells for the Development of Cultured Meat |
| title_sort | effects of hypoxia on proliferation and differentiation in belgian blue and hanwoo muscle satellite cells for the development of cultured meat |
| topic | hypoxia normoxia bovine satellite cell proliferation differentiation |
| url | https://www.mdpi.com/2218-273X/12/6/838 |
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