Effect of Cerenkov Radiation-Induced Photodynamic Therapy with <sup>18</sup>F-FDG in an Intraperitoneal Xenograft Mouse Model of Ovarian Cancer
Ovarian cancer (OC) metastases frequently occur through peritoneal dissemination, and they contribute to difficulties in treatment. While photodynamic therapy (PDT) has the potential to treat OC, its use is often limited by tissue penetration depth and tumor selectivity. Herein, we combined Cerenkov...
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MDPI AG
2021-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/9/4934 |
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| author | Yi-An Chen Jia-Je Li Syue-Liang Lin Cheng-Hsiu Lu Sain-Jhih Chiu Fong-Shya Jeng Chi-Wei Chang Bang-Hung Yang Ming-Cheng Chang Chien-Chih Ke Ren-Shyan Liu |
| author_facet | Yi-An Chen Jia-Je Li Syue-Liang Lin Cheng-Hsiu Lu Sain-Jhih Chiu Fong-Shya Jeng Chi-Wei Chang Bang-Hung Yang Ming-Cheng Chang Chien-Chih Ke Ren-Shyan Liu |
| author_sort | Yi-An Chen |
| collection | DOAJ |
| description | Ovarian cancer (OC) metastases frequently occur through peritoneal dissemination, and they contribute to difficulties in treatment. While photodynamic therapy (PDT) has the potential to treat OC, its use is often limited by tissue penetration depth and tumor selectivity. Herein, we combined Cerenkov radiation (CR) emitted by <sup>18</sup>F-FDG accumulated in tumors as an internal light source and several photosensitizer (PS) candidates with matched absorption bands, including Verteporfin (VP), Chlorin e6 (Ce6) and 5′-Aminolevulinic acid (5′-ALA), to evaluate the anti-tumor efficacy. The in vitro effect of CR-induced PDT (CR-PDT) was evaluated using a cell viability assay, and the efficiency of PS was assessed by measuring the singlet oxygen production. An intraperitoneal ES2 OC mouse model was used for in vivo evaluation of CR-PDT. Positron emission tomography (PET) imaging and bioluminescence-based imaging were performed to monitor the biologic uptake of <sup>18</sup>F-FDG and the therapeutic effect. The in vitro studies demonstrated Ce6 and VP to be more effective PSs for CR-PDT. Moreover, VP was more efficient in the generation of singlet oxygen and continued for a long time when exposed to fluoro-18 (<sup>18</sup>F). Combining CR emitted by <sup>18</sup>F-FDG and VP treatment not only significantly suppressed tumor growth, but also prolonged median survival times compared to either monotherapy. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T11:39:49Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5971c2f6e3cf4c2493751b85472444c62023-11-21T18:33:59ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-05-01229493410.3390/ijms22094934Effect of Cerenkov Radiation-Induced Photodynamic Therapy with <sup>18</sup>F-FDG in an Intraperitoneal Xenograft Mouse Model of Ovarian CancerYi-An Chen0Jia-Je Li1Syue-Liang Lin2Cheng-Hsiu Lu3Sain-Jhih Chiu4Fong-Shya Jeng5Chi-Wei Chang6Bang-Hung Yang7Ming-Cheng Chang8Chien-Chih Ke9Ren-Shyan Liu10Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei 112, TaiwanDepartment of Biomedical Imaging and Radiological Sciences, National Yang Ming Chiao Tung University, Taipei 112, TaiwanDepartment of Biomedical Imaging and Radiological Sciences, National Yang Ming Chiao Tung University, Taipei 112, TaiwanMolecular and Genetic Imaging Core/Taiwan Mouse Clinic, National Comprehensive Mouse Phenotyping and Drug Testing Center, Taipei 112, TaiwanMolecular and Genetic Imaging Core/Taiwan Mouse Clinic, National Comprehensive Mouse Phenotyping and Drug Testing Center, Taipei 112, TaiwanMolecular and Genetic Imaging Core/Taiwan Mouse Clinic, National Comprehensive Mouse Phenotyping and Drug Testing Center, Taipei 112, TaiwanNational PET and Cyclotron Center (NPCC), Department of Nuclear Medicine, Taipei Veterans General Hospital, Taipei 112, TaiwanDepartment of Biomedical Imaging and Radiological Sciences, National Yang Ming Chiao Tung University, Taipei 112, TaiwanInstitute of Nuclear Energy Research, Atomic Energy Council, Executive Yuan, Taoyuan County 325, TaiwanDepartment of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, TaiwanInstitute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei 112, TaiwanOvarian cancer (OC) metastases frequently occur through peritoneal dissemination, and they contribute to difficulties in treatment. While photodynamic therapy (PDT) has the potential to treat OC, its use is often limited by tissue penetration depth and tumor selectivity. Herein, we combined Cerenkov radiation (CR) emitted by <sup>18</sup>F-FDG accumulated in tumors as an internal light source and several photosensitizer (PS) candidates with matched absorption bands, including Verteporfin (VP), Chlorin e6 (Ce6) and 5′-Aminolevulinic acid (5′-ALA), to evaluate the anti-tumor efficacy. The in vitro effect of CR-induced PDT (CR-PDT) was evaluated using a cell viability assay, and the efficiency of PS was assessed by measuring the singlet oxygen production. An intraperitoneal ES2 OC mouse model was used for in vivo evaluation of CR-PDT. Positron emission tomography (PET) imaging and bioluminescence-based imaging were performed to monitor the biologic uptake of <sup>18</sup>F-FDG and the therapeutic effect. The in vitro studies demonstrated Ce6 and VP to be more effective PSs for CR-PDT. Moreover, VP was more efficient in the generation of singlet oxygen and continued for a long time when exposed to fluoro-18 (<sup>18</sup>F). Combining CR emitted by <sup>18</sup>F-FDG and VP treatment not only significantly suppressed tumor growth, but also prolonged median survival times compared to either monotherapy.https://www.mdpi.com/1422-0067/22/9/4934ovarian cancerphotodynamic therapyCerenkov radiation<sup>18</sup>F-FDGphotosensitizer |
| spellingShingle | Yi-An Chen Jia-Je Li Syue-Liang Lin Cheng-Hsiu Lu Sain-Jhih Chiu Fong-Shya Jeng Chi-Wei Chang Bang-Hung Yang Ming-Cheng Chang Chien-Chih Ke Ren-Shyan Liu Effect of Cerenkov Radiation-Induced Photodynamic Therapy with <sup>18</sup>F-FDG in an Intraperitoneal Xenograft Mouse Model of Ovarian Cancer International Journal of Molecular Sciences ovarian cancer photodynamic therapy Cerenkov radiation <sup>18</sup>F-FDG photosensitizer |
| title | Effect of Cerenkov Radiation-Induced Photodynamic Therapy with <sup>18</sup>F-FDG in an Intraperitoneal Xenograft Mouse Model of Ovarian Cancer |
| title_full | Effect of Cerenkov Radiation-Induced Photodynamic Therapy with <sup>18</sup>F-FDG in an Intraperitoneal Xenograft Mouse Model of Ovarian Cancer |
| title_fullStr | Effect of Cerenkov Radiation-Induced Photodynamic Therapy with <sup>18</sup>F-FDG in an Intraperitoneal Xenograft Mouse Model of Ovarian Cancer |
| title_full_unstemmed | Effect of Cerenkov Radiation-Induced Photodynamic Therapy with <sup>18</sup>F-FDG in an Intraperitoneal Xenograft Mouse Model of Ovarian Cancer |
| title_short | Effect of Cerenkov Radiation-Induced Photodynamic Therapy with <sup>18</sup>F-FDG in an Intraperitoneal Xenograft Mouse Model of Ovarian Cancer |
| title_sort | effect of cerenkov radiation induced photodynamic therapy with sup 18 sup f fdg in an intraperitoneal xenograft mouse model of ovarian cancer |
| topic | ovarian cancer photodynamic therapy Cerenkov radiation <sup>18</sup>F-FDG photosensitizer |
| url | https://www.mdpi.com/1422-0067/22/9/4934 |
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