Epigallocatechin-3-Gallate Protects Erythrocyte Ca2+-ATPase and Na+/K+-ATPase Against Oxidative Induced Damage During Aging in Humans
Purpose: The main purpose of this study was to investigate the protective role of epigallocatechin-3-gallate on tertiary butyl hydroperoxide induced oxidative damage in erythrocyte during aging in humans. Methods: Human erythrocyte membrane bound Ca2+-ATPase and Na+/K+-ATPase activities were determ...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2014-10-01
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| Series: | Advanced Pharmaceutical Bulletin |
| Subjects: | |
| Online Access: | http://apb.tbzmed.ac.ir/Portals/0/Archive/Vol4NoSupp1/5-Supp1-Maurya.pdf |
| _version_ | 1819141300603060224 |
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| author | Prabhanshu Kumar Pawan Kumar Maurya |
| author_facet | Prabhanshu Kumar Pawan Kumar Maurya |
| author_sort | Prabhanshu Kumar |
| collection | DOAJ |
| description | Purpose: The main purpose of this study was to investigate the protective role of epigallocatechin-3-gallate on tertiary butyl hydroperoxide induced oxidative damage in erythrocyte during aging in humans.
Methods: Human erythrocyte membrane bound Ca2+-ATPase and Na+/K+-ATPase activities were determined as a function of human age. Protective role of epigallocatechin-3-gallate was evaluated by in vitro experiments by adding epigallocatechin-3-gallate in concentration dependent manner (final concentration range 10-7M to 10-4M) to the enzyme assay medium. Oxidative stress was induced in vitro by incubating washed erythrocyte ghosts with tertiary butyl hydroperoxide (10-5 M final concentration).
Results: We have reported concentration dependent effect of epigallocatechin-3-gallate on tertiary butyl hydroperoxide induced damage on activities of Ca2+-ATPase and Na+/K+-ATPase during aging in humans. We have detected a significant (p < 0.001) decreased activity of Ca2+-ATPase and Na+/K+ -ATPase as a function of human age. Epigallocatechin-3-gallate protected ATPases against tertiary butyl hydroperoxide induced damage in concentration dependent manner during aging in humans.
Conclusion: Epigallocatechin-3-gallate is a powerful antioxidant that is capable of protecting erythrocyte Ca2+-ATPase and Na+/K+ -ATPase against oxidative stress during aging in humans. We may propose hypothesis that a high intake of catechin rich diet may provide some protection against development of aging and age related diseases. |
| first_indexed | 2024-12-22T11:52:16Z |
| format | Article |
| id | doaj.art-5976146d48b748adacd23aaa227d9c09 |
| institution | Directory Open Access Journal |
| issn | 2228-5881 2251-7308 |
| language | English |
| last_indexed | 2024-12-22T11:52:16Z |
| publishDate | 2014-10-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Advanced Pharmaceutical Bulletin |
| spelling | doaj.art-5976146d48b748adacd23aaa227d9c092022-12-21T18:26:57ZengTabriz University of Medical SciencesAdvanced Pharmaceutical Bulletin2228-58812251-73082014-10-014suppl 144344710.5681/apb.2014.065Epigallocatechin-3-Gallate Protects Erythrocyte Ca2+-ATPase and Na+/K+-ATPase Against Oxidative Induced Damage During Aging in HumansPrabhanshu Kumar0Pawan Kumar Maurya1Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida, 201303, India.Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida, 201303, India.Purpose: The main purpose of this study was to investigate the protective role of epigallocatechin-3-gallate on tertiary butyl hydroperoxide induced oxidative damage in erythrocyte during aging in humans. Methods: Human erythrocyte membrane bound Ca2+-ATPase and Na+/K+-ATPase activities were determined as a function of human age. Protective role of epigallocatechin-3-gallate was evaluated by in vitro experiments by adding epigallocatechin-3-gallate in concentration dependent manner (final concentration range 10-7M to 10-4M) to the enzyme assay medium. Oxidative stress was induced in vitro by incubating washed erythrocyte ghosts with tertiary butyl hydroperoxide (10-5 M final concentration). Results: We have reported concentration dependent effect of epigallocatechin-3-gallate on tertiary butyl hydroperoxide induced damage on activities of Ca2+-ATPase and Na+/K+-ATPase during aging in humans. We have detected a significant (p < 0.001) decreased activity of Ca2+-ATPase and Na+/K+ -ATPase as a function of human age. Epigallocatechin-3-gallate protected ATPases against tertiary butyl hydroperoxide induced damage in concentration dependent manner during aging in humans. Conclusion: Epigallocatechin-3-gallate is a powerful antioxidant that is capable of protecting erythrocyte Ca2+-ATPase and Na+/K+ -ATPase against oxidative stress during aging in humans. We may propose hypothesis that a high intake of catechin rich diet may provide some protection against development of aging and age related diseases.http://apb.tbzmed.ac.ir/Portals/0/Archive/Vol4NoSupp1/5-Supp1-Maurya.pdfErythrocytesAgingCa2+-ATPaseNa+/K+ -ATPaseEGCGOxidative stress |
| spellingShingle | Prabhanshu Kumar Pawan Kumar Maurya Epigallocatechin-3-Gallate Protects Erythrocyte Ca2+-ATPase and Na+/K+-ATPase Against Oxidative Induced Damage During Aging in Humans Advanced Pharmaceutical Bulletin Erythrocytes Aging Ca2+-ATPase Na+/K+ -ATPase EGCG Oxidative stress |
| title | Epigallocatechin-3-Gallate Protects Erythrocyte Ca2+-ATPase and Na+/K+-ATPase Against Oxidative Induced Damage During Aging in Humans |
| title_full | Epigallocatechin-3-Gallate Protects Erythrocyte Ca2+-ATPase and Na+/K+-ATPase Against Oxidative Induced Damage During Aging in Humans |
| title_fullStr | Epigallocatechin-3-Gallate Protects Erythrocyte Ca2+-ATPase and Na+/K+-ATPase Against Oxidative Induced Damage During Aging in Humans |
| title_full_unstemmed | Epigallocatechin-3-Gallate Protects Erythrocyte Ca2+-ATPase and Na+/K+-ATPase Against Oxidative Induced Damage During Aging in Humans |
| title_short | Epigallocatechin-3-Gallate Protects Erythrocyte Ca2+-ATPase and Na+/K+-ATPase Against Oxidative Induced Damage During Aging in Humans |
| title_sort | epigallocatechin 3 gallate protects erythrocyte ca2 atpase and na k atpase against oxidative induced damage during aging in humans |
| topic | Erythrocytes Aging Ca2+-ATPase Na+/K+ -ATPase EGCG Oxidative stress |
| url | http://apb.tbzmed.ac.ir/Portals/0/Archive/Vol4NoSupp1/5-Supp1-Maurya.pdf |
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